Effect of domestic porous tantalum modified by osteogenic induction factor slow-release system on function of MG63 cells

doi:10.12307/2024.410

Abstract

Abstract: BACKGROUND: Previous research by the research team found that domestically produced porous tantalum is beneficial for early adhesion and proliferation of MG63 cells, and can be used as a scaffold material for bone tissue engineering.
OBJECTIVE: To investigate the effect of domestic porous tantalum modified by osteogenic induction factor slow-release system on the adhesion, proliferation, and differentiation of MG63 cells.
METHODS: Osteogenic induction factor slow-release system was constructed by adding 15% volume fraction of osteogenic factor solution to poly(lactic-co-glycolic-acid) gel. The passage 3 MG63 cells were inoculated on a porous tantalum surface (control group), porous tantalum surface coated with poly(lactic-co-glycolic-acid) copolymer gel (gel group), and porous tantalum surface coated with osteoblastic induction factor slow-release system (slow-release system group), and co-cultured for 5 days. The surface cytoskeleton of the material was observed by phalloidine staining. Cell proliferation was detected by flow cytometry. Western blot assay and RT-qPCR were used to detect the protein and mRNA expressions of type I collagen, osteopontin, and RUNX-2 on the surface cells of the material.
RESULTS AND CONCLUSION: (1) Phalloidine staining showed that MG63 cells adhered to and grew on the surface and inside of the three groups of porous tantalum, and the matrix secreted by the cells covered the surface of the material. (2) Flow cytometry showed that the cell proliferation in the slow-release system group was faster than that in the control group and the gel group (P < 0.05). (3) Western blot assay and RT-qPCR showed that the protein and mRNA expressions of type I collagen, osteopontin, and RUNX-2 in the slow-release system group were higher than those in the control group and gel group (P < 0.05). (4) The results showed that the domestic porous tantalum modified by the osteogenic induction factor slow-release system was beneficial to the adhesion, proliferation, and differentiation of MG63 osteoblasts.

Key words: porous tantalum, MG63 cell, slow-release system, osteogenic induction factor, poly(lactic-co-glycolic-acid) copolymer

CLC Number:

Guo Xiaoling, Li Yueyuan, Xu Tianjie, Zhang Hui, Wang Zhiqiang, Wang Qian. Effect of domestic porous tantalum modified by osteogenic induction factor slow-release system on function of MG63 cells[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(17): 2696-2701.

Figures/Tables 8

References

[1] KASHIRINA A, YAO Y, LIU Y, et al. Biopolymers as bone substitutes: A review. Biomater Sci. 2019;7(10):3961-3983.
[2] ZHANG Z, LI Y, HE P, et al. Nanotube-decorated hierarchical tantalum scaffold promoted early osseointegration. Nanomedicine. 2021;35:102390.
[3] MA L, CHENG S, JI X, et al. Immobilizing magnesium ions on 3D printed porous tantalum scaffolds with polydopamine for improved vascularization and osteogenesis. Mater Sci Eng C Mater Biol Appl C. 2020;117:111303.
[4] LI JJ, EBIED M, XU J, et al. Current approaches to bone tissue engineering: The interface between biology and engineering. Adv Healthc Mater. 2018;7(6): e1701061.
[5] CHENG CY, PHO QH, WU XY, et al. PLGA microspheres loaded with β-cyclodextrin complexes of epigallocatechin-3-gallate for the anti-inflammatory properties in activated microglial cells. Polymers. 2018;10(5):519.
[6] SHI NQ, ZHOU J, WALKER J, et al. Microencapsulation of luteinizing hormone-releasing hormone agonist in poly (lactic-co-glycolic acid) microspheres by spray-drying. J Control Release. 2020;321:756-772.
[7] MA C, ZHANG Y, JIAO Z, et al. A nanocarrier based on poly (d, l-lactic-co-glycolic acid) for transporting Na+ and Cl− to induce apoptosis. Chin Chem Lett. 2020;31(6):1635-1639.
[8] SU Y, ZHANG B, SUN R, et al. PLGA-based biodegradable microspheres in drug delivery: recent advances in research and application. Drug Deliv. 2021;28(1): 1397-1418.
[9] UZER-YILMAZ B. In vitro contact guidance of glioblastoma cells on metallic biomaterials. J Mater Sci Mater Med. 2021;32(4):35.
[10] GIRÓN J, KERSTNER E, MEDEIROS T, et al. Biomaterials for bone regeneration: an orthopedic and dentistry overview. Braz J Med Biol Res. 2021;54(9):e11055.
[11] KANG C, WEI L, SONG B, et al. Involvement of autophagy in tantalum nanoparticle-induced osteoblast proliferation. Int J Nanomedicine. 2017;12: 4323.
[12] FAN H, DENG S, TANG W, et al. Highly porous 3D printed tantalum scaffolds have better biomechanical and microstructural properties than titanium scaffolds. Biomed Res Int. 2021;2021:2899043.
[13] Zhao D, MA Z, WANG T, et al. Biocompatible porous tantalum metal plates in the treatment of tibial fracture. Orthop Surg. 2019;11(2):325-329.
[14] HUANG G, PAN ST, QIU JX. The clinical application of porous tantalum and its new development for bone tissue engineering. Materials. 2021;14(10):2647.
[15] BAKRI MM, LEE SH, LEE JH. Improvement of biohistological response of facial implant materials by tantalum surface treatment. Maxillofac Plast Reconstr Surg. 2019;41(1):1-8.
[16] Gracia MAA. Osseoincorporation of porous tantalum trabecular-structured metal: a histologic and histomorphometric study in humans. Int J Periodontics Restorative Dent. 2018;38:879-885.
[17] MAJHY B, PRIYADARSHINI P, SEN AK. Effect of surface energy and roughness on cell adhesion and growth–facile surface modification for enhanced cell culture. RSC Adv. 2021;11(25):15467-15476.
[18] HUANG G, PAN ST, QIU JX. The osteogenic effects of porous Tantalum and Titanium alloy scaffolds with different unit cell structure. Colloids Surf B Biointerfaces. 2022;210:112229.
[19] GEE ECA, ELEOTÉRIO R, BOWKER LM, et al. The influence of tantalum on human cell lineages important for healing in soft-tissue reattachment surgery: an in-vitro analysis. J Exp Orthop. 2019;6(1):1-6.
[20] PIGLIONICO S, BOUSQUET J, FATIMA N, et al. Porous tantalum vs. titanium implants: enhanced mineralized matrix formation after stem cells proliferation and differentiation. J Clin Med. 2020;9(11):3657.
[21] COELHO MJ, FERNANDES MH. Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, β-glycerophosphate and dexamethasone on osteoblastic differentiation. Biomaterials. 2000;21(11):1095-1102.
[22] DANHIER F, ANSORENA E, SILVA JM, et al. PLGA-based nanoparticles: an overview of biomedical application. J Control Release. 2012;161(2):505-522.
[23] TRUONG T, JONES KS. Capsaicin reduces PLGA‐induced fibrosis by promoting M2 macrophages and suppressing overall inflammatory Response. J Biomed Mater Res A. 2018;106(9):2424-2432.
[24] SOLHEIM E, SUDMANN B, BANG G, et al. Biocompatibility and effect on osteogenesis of poly (ortho ester) compared to poly (DL‐lactic acid). J Biomed Mater Res. 2000;49(2):257-263.
[25] WANG T, GUO S, ZHANG H. Synergistic effects of controlled-released BMP-2 and VEGF from nHAC/PLGAs scaffold on osteogenesis. Biomed Res Int. 2018;2018: 3516463.
[26] XIA P, WANG S, QI Z, et al. BMP-2-releasing gelatin microspheres/PLGA scaffolds for bone repairment of X-ray-radiated rabbit radius defects. Artif Cells Nanomed Biotechnol. 2019;47(1):1662-1673.
[27] SENADHEERA TRL, DAVE D, SHAHIDI F. Sea cucumber derived type I collagen: A comprehensive review. Mar Drugs. 2020;18(9):471.
[28] SIMS NA, VRAHNAS C. Regulation of cortical and trabecular bone mass by communication between osteoblasts, osteocytes and osteoclasts. Arch Biochem Biophys. 2014;561:22-28.
[29] MARELLI B, GHEZZI CE, BARRALET JE, et al. Three-dimensional mineralization of dense nanofibrillar collagen-bioglass hybrid scaffolds. Biomacromolecules. 2010;11(6):1470-1479.
[30] KIM LT, YAMADA KM. The regulation of expression of integrin receptors. Proc Soc Exp Biol Med. 1997;214(2):123-131.
[31] CHAN BPH, VINCENT K, LAJOIE GA, et al. On the catalysis of calcium oxalate dihydrate formation by osteopontin peptides. Colloids Surf B Biointerfaces. 2012;96:22-28.
[32] DAI B, XU J, LI X, et al. Macrophages in epididymal adipose tissue secrete osteopontin to regulate bone homeostasis. Nat Commun. 2022;13(1):427.
[33] DENHARDT DT, NODA M. Osteopontin expression and function: role in bone remodeling. J Cell Biochem Suppl. 1998;72(S30-31):92-102.
[34] LENTON S, SEYDEL T, NYLANDER T, et al. Dynamic footprint of sequestration in the molecular fluctuations of osteopontin. J R Soc Interface. 2015;12(110):0506.
[35] KOMORI T. Runx2, an inducer of osteoblast and chondrocyte differentiation. Histochem Cell Biol. 2018;149(4):313-323.
[36] HOU Z, WANG Z, TAO Y, et al. KLF2 regulates osteoblast differentiation by targeting of Runx2. Lab Invest. 2019;99(2):271-280.
[37] NARAYANAN A, SRINAATH N, ROHINI M, et al. Regulation of Runx2 by MicroRNAs in osteoblast differentiation. Life Sci. 2019;232:116676.
[38] DONG M, JIAO G, LIU H, et al. Biological silicon stimulates collagen type 1 and osteocalcin synthesis in human osteoblast-like cells through the BMP-2/Smad/RUNX2 signaling pathway. Biol Trace Elem Res. 2016;173:306-315.
[39] CHEN J, LV D, PAN Q, et al. Local application of the osteogenic inducer sustained-release system promotes early bone remodeling around titanium implants. Int J Oral Maxillofac Surg. 2022;51(4):558-565.
[40] CAI Q, SHI G, BEI J, et al. Enzymatic degradation behavior and mechanism of poly (lactide-co-glycolide) foams by trypsin. Biomaterials. 2003;24(4):629-638.
[41] MEYER F, WARDALE J, BEST S, et al. Effects of lactic acid and glycolic acid on human osteoblasts: a way to understand PLGA involvement in PLGA/calcium phosphate composite failure. J Orthop Res. 2012;30(6):864-871.
[42] LIU X, CHEN J, LUO Y, et al. Osteogenic inducer sustained-release system promotes the adhesion, proliferation, and differentiation of osteoblasts on titanium surface. Ann Anat. 2020;231:151523.
[43] 刘旭琳,张潇月,田欣,等.成骨诱导剂缓释系统对拔牙创骨改建的影响[J].安徽医科大学学报,2021,56(5):823-827.