Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (1): 20-26.doi: 10.12307/2022.004
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Xie Xingqi, Hu Wei, Tu Guanjun
Received:
2021-02-05
Revised:
2021-02-07
Accepted:
2021-03-13
Online:
2022-01-08
Published:
2021-10-23
Contact:
Tu Guanjun, MD, Professor, Chief physician, Department of Orthopedics, First Affiliated Hospital of China Medical University, Shenyang 110000, Liaoning Province, China
About author:
Xie Xingqi, Master candidate, Department of Orthopedics, First Affiliated Hospital of China Medical University, Shenyang 110000, Liaoning Province, China
Supported by:
CLC Number:
Xie Xingqi, Hu Wei, Tu Guanjun. Bone marrow mesenchymal stem cells-derived exosomes combined with chondroitinase ABC for treating spinal cord injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(1): 20-26.
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2.3 后肢运动功能评分 脊髓损伤后1 d各组大鼠后肢运动功能完全丧失,3 d后逐渐不同程度恢复,见图3。联合组脊髓损伤后7 d可见双后肢多关节节律运动,14 d可见掌面承重、后肢负重位,28 d可见后肢协调运动,见图3。各组脊髓损伤后1,3 d的BBB评分差异均无显著性意义(P > 0.05)。脊髓损伤后第7,14,28天,ChABC组、外泌体组、联合组BBB评分显著高于模型组,差异均有显著性意义(P < 0.05),说明治疗均有效。联合组BBB评分显著高于ChABC组、外泌体组,差异均有显著性意义(P < 0.05)。ChABC组和外泌体组间BBB评分差异无显著性意义(P > 0.05),见表1。结果说明,BMSCs-Exo联合ChABC能协同发挥作用,促进脊髓损伤大鼠后肢运动功能恢复。"
2.5 免疫荧光检测胶质纤维酸性蛋白表达 各组均有胶质纤维酸性蛋白阳性表达,主要集中于脊髓损伤区边缘。模型组胶质纤维酸性蛋白呈条索状广泛分布;ChABC组、外泌体组有少量呈条索状或星点状胶质纤维酸性蛋白阳性表达;联合组仅有少量星点状胶质纤维酸性蛋白表达,见图5A。与模型组、ChABC组、外泌体组比较,联合组胶质纤维酸性蛋白表达显著降低,差异有显著性意义(P < 0.05);与模型组比较,ChABC组、外泌体组胶质纤维酸性蛋白表达显著降低(P < 0.05);与外泌体组比较,ChABC组胶质纤维酸性蛋白表达显著降低(P < 0.05),见图5B。结果说明,BMSCs-Exo联合ChABC能减少大鼠脊髓损伤后胶质瘢痕的形成,有利于神经元和轴突再生。"
2.6 免疫组化检测生长相关蛋白43表达 各组均可见生长相关蛋白43阳性细胞,见图6A。联合组生长相关蛋白43显著表达,与其余各组比较,差异有显著性意义(P < 0.05)。与模型组比较,ChABC组、外泌体组生长相关蛋白43表达增加(P < 0.05);与ChABC组比较,外泌体组生长相关蛋白43表达亦增加(P < 0.05),见图6B。结果说明,BMSCs-Exo联合ChABC能显著提高大鼠脊髓损伤后生长相关蛋白43表达,从而促进损伤神经元和轴突的修复与再生。 2.7 生物相容性 BMSCs-Exo和ChABC注射入大鼠模型后,未见伤口延迟愈合、感染、免疫排斥反应等不良影响,具有良好的生物相容性和转化意义。"
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