Protective mechanism of Gushukang granule in a rat osteoporosis model based on TMT proteomic analysis

doi:10.12307/2021.216

Abstract

Abstract: BACKGROUND: The specific mechanism and target of Gushukang in the treatment of osteoporosis are still unclear, which need further explorations.
OBJECTIVE: To explore the protective mechanism of Gushukang on bone metabolism in osteoporotic rats and to analyze the differentially expressed proteins by bioinformatics.
METHODS: Twenty-four osteoporosis model rats were established by ovariectomy, 12 in model group and 12 in Gushukang group. Another 12 rats without ovariectomy were used as sham operation group. Gushukang capsule, 4.32 g/kg/d, was intragastrically administered once a day in the Gushukang group, and rats in the other two groups were given the same volume of normal saline. Treatment in each group lasted for 3 months. Bone mineral density of the tibia was measured. The proteomic analysis of the lumbar vertebrae of osteoporotic rats was carried out by TMT-LC-MC/MC technique, and the differentially expressed proteins were screened, analyzed by gene ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and protein-protein interaction, thereby constructing a protein-protein interaction network. An ethical approval was obtained from the Animal Ethics Committee of Fujian Academy of Traditional Chinese Medicine.
RESULTS AND CONCLUSION: A total of 32 up-regulated and 49 down-regulated proteins were screened between Gushukang group and model group. Gene ontology enrichment analysis showed that these differentially expressed proteins were involved in biological processes, such as immunoglobulin receptor binding and vascular endothelial growth factor receptor binding, and molecular functions, such as nucleic acid template transcription and RNA biosynthesis. KEGG enrichment analysis showed that the differentially expressed proteins in the Gushukang group and model group were mainly involved in tyrosine metabolism, ethanol metabolism, JAK-STAT and other signal pathways. Protein-protein interaction analysis showed that among the common differential proteins in the Gushukang group and model group, Mecp2, Aldh3b1, Ryr1 and Myoz1 were located at the nodes of protein interaction network and were closely related to bone metabolism. These findings indicate that Gushukang granule may be involved in protecting the bone metabolism of osteoporotic rats by regulating differential proteins, tyrosine metabolism, JAK-STAT and other important signal pathways.

Key words: Gushukang, TMT technology, proteomics, differential protein, rat osteoporosis model, bone metabolism, bioinformatics, traditional Chinese medicine

CLC Number:

Lin Ruohui, Chen Sainan, Ye Yunjin, Chen Juan, Xie Lihua, Huang Jingwen, Ge Jirong, Li Shengqiang. Protective mechanism of Gushukang granule in a rat osteoporosis model based on TMT proteomic analysis[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(32): 5141-5147.

Figures/Tables 9

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