中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 855-858.doi: 10.3969/j.issn.1673-8225.2012.05.023

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

不同氧体积分数气体对体外模拟体内生理环境寄养断肢系统血氧分压的影响☆

王江宁，尹叶锋，高  磊，王德成，左有为，苏才培   

  1. 首都医科大学潞河教学医院，北京市  101100
  • 收稿日期:2011-07-23 修回日期:2011-08-29 出版日期:2012-01-29 发布日期:2012-01-29
  • 作者简介:王江宁☆，男，1958年生，江苏省南京市人，汉族，2007年大连理工大学毕业，博士，主任医师，主要从事修复重建显微外科方面的研究。dlwangjn@vip.sina.com

Effect of different oxygen concentrations on blood oxygen pressure in the system of in vitro physiological environment foster limbs

Wang Jiang-ning, Yin Ye-feng, Gao Lei, Wang De-cheng, Zuo You-wei, Su Cai-pei   

  1. Luhe Teaching Hospital, Capital Medical University, Beijing  101100, China
  • Received:2011-07-23 Revised:2011-08-29 Online:2012-01-29 Published:2012-01-29
  • About author:Wang Jiang-ning☆, Doctor, Chief physician, Luhe Teaching Hospital, Capital Medical University, Beijing 101100, China dlwangjn@vip.sina.com

PDF

477

摘要：

背景：断肢再植成功率与断肢的保存方法有直接关系，开展体外模拟体内生理环境寄养断肢的研究，可以延长肢体保存时间。
目的：探讨不同氧体积分数对体外模拟体内生理环境寄养断肢系统血氧分压的影响。
方法：健康的新西兰绵羊9头，共计36只肢体，行体外断肢同型血液循环灌注实验。36只肢体按照进入膜肺的气体氧体积分数的不同随机数字表均分成氧体积分数100%，60%，50%，40%，20%，10%组进行观察。
结果与结论：各组血氧分压数据相比较，氧体积分数10%的动脉血氧分压数值在正常值范围(10.64~13.33 kPa)，而且动脉血氧分压的数值随氧体积分数的减小而减小。提示在体外模拟体内生理环境寄养断肢系统中，不同氧体积分数的气体进入膜肺可以对动脉血氧分压产生影响，减小氧体积分数可以使动脉血氧分压接近于正常范围。

关键词: 断肢, 氧体积分数, 体外寄养, 模拟人体生理环境, 血氧分压

Abstract:

BACKGROUND: Limb replantation success rate with limb preservation method is directly related to conduct in vitro simulated physiological environment foster limbs, which can extend saved time of the limbs.
OBJECTIVE: To investigate the effects of different oxygen concentrations on blood oxygen pressure in the system of in vitro physiological environment foster limbs.
METHODS: Totally nine New Zealand sheep with a total of 36 limbs underwent in vitro limb perfusion experiments with blood and were randomly divided into six groups according to different concentrations of oxygen gas (100%, 60%, 50%, 40%, 20%, 10%) into the membrane lung.
RESULTS AND CONCLUSION: Comparison of blood oxygen pressure in each group, the arterial oxygen values in experimental group 6 was in the normal range (80-100 mm Hg), and with the oxygen concentration decreases, arterial oxygen pressure values decreases. It is indicated that in vitro simulation of physiological environment in the foster care system, different concentrations of oxygen gas into the membrane lung may have an impact on the arterial partial pressure of oxygen; through reducing the oxygen concentration, the arterial oxygen pressure can be close to the normal range.

中图分类号: