中国组织工程研究

中国组织工程研究

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

纯化取材脂肪提高实验中干细胞培养的纯度

宋小飞1，傅  强2   

  1. 1上海瑞金集团闵行中心医院泌尿外科，上海市 201100
    2上海交通大学附属第六人民医院泌尿外科，上海市200233
  • 收稿日期:2011-02-22 修回日期:2011-05-04 出版日期:2011-10-01 发布日期:2011-10-01
  • 通讯作者: 傅强，教授,博士生导师，上海交通大学附属第六人民医院泌尿外科，上海市 200233 Jamesqiangfu@yahoo.com.cn
  • 作者简介:宋小飞★，1982年生，安徽省合肥市人，汉族，2009年上海交通大学医学院毕业，硕士，医师，主要从事组织工程技术在泌尿外科运用方面的研究。 sxfonline@qq.com

Raising purity of adipose tissue-derived stem cells by purifying adipose tissue in the laboratory

Song Xiao-fei1, Fu Qiang2   

  1. 1Department of Urology, Minhang District Center Hospital, Ruijing Hospital, Shanghai  201100, China
    2Department of Urology, Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai  200233, China
  • Received:2011-02-22 Revised:2011-05-04 Online:2011-10-01 Published:2011-10-01
  • Contact: Fu Qiang, Professor, Doctoral supervisor, Department of Urology, Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China Jamesqiangfu@yahoo.com.cn
  • About author:Song Xiao-fei★, Master, Physician, Department of Urology, Minhang District Center Hospital, Ruijing Hospital, Shanghai 201100, China sxfonline@qq.com

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289

被引次数

3

摘要：

背景：在以往脂肪干细胞原代培养的文献中大多只是剔除脂肪表面的血管组织，而对于脂肪内部的血管，尤其是血管周围淋巴结的剔除很少有文献报道。
目的：探讨通过脂肪纯化来提高培养干细胞纯度的可行性。
方法：将取材自同一大鼠腹股沟处的脂肪组织分成两份，一份给予纯化处理，剔除表面的血管、皮肤和肌肉组织的同时一并剔除组织内血管及其周围结节样组织。而另一份仅剔除表面血管及可能黏附的皮肤和肌肉组织，分别对两份脂肪行干细胞原代培养。
结果与结论：经苏木精-伊红染色显微镜下观察，证实血管周围结节样组织为淋巴结。纯化脂肪组培养的细胞形态一致、呈长梭形。脂肪干细胞相关性抗原CD90的免疫荧光和流式细胞仪检测结果均证明纯化脂肪组培养的细胞纯度较未纯化组高。实验结果提示通过纯化取材可以提高原代培养的脂肪干细胞纯度。

关键词: 脂肪组织, 干细胞, 淋巴结, 细胞纯化, 组织工程

Abstract:

BACKGROUND: In the past, only vascular tissues on fat surface were removed during the primary culture of adipose tissue-derived stem cells (ADSCs) reported in most literatures. However, blood vessels within the fat tissues, especially the lymph nodes around the blood vessels removed little.
OBJECTIVE: To investigate the feasibility of raising purity of cultured ADSCs in the laboratory through purifying adipose tissues.
METHODS: Adipose tissues derived from rat inguinal groove were divided into two parts: one for purifying, rejecting superficial blood vessel, skins and muscular tissues, rejecting blood vessel inside and surrounding elliptic nodal tissues; the other one only for rejecting superficial blood vessels and possible adherent skins and muscular tissues outside. Primary culture of ADSCs was performed.
RESULTS AND CONCLUSION: Elliptic nodal tissues stained by hematoxylin-eosin were proved to be lymphatic tissues under microscope. The cells cultured from purified adipose tissues were uniformity. Cell photos, immunofluorescence and flow cytometry results can prove that the cell’s purity from the purified adipose tissues was higher than that from the unpurified adipose tissues. The purity of primary cultured ADSCs can be improved by purifying adipose tissues in the laboratory.

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