中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (43): 8023-8026.doi: 10.3969/j.issn.2095-4344.2012.43.010

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米银缓释壳聚糖/聚乙烯醇伤口敷料的制备及表征

许为康1,2,3，魏 坤1,2,3，曾晓峰1,2,3，凌 友1,2,3，张 捷1,2,3，魏欣苗1,2,3   

  1. 1华南理工大学材料科学与工程学院，广东省广州市 510640
    2国家人体组织功能重建工程技术研究中心，广东省广州市 510006
    3广东省生物医学工程重点实验室，广东省广州市 510006
  • 收稿日期:2012-01-19 修回日期:2012-03-12 出版日期:2012-10-21 发布日期:2013-04-08
  • 通讯作者: 魏坤，博士，教授，华南理工大学材料科学与工程学院，广东省广州市510640；国家人体组织功能重建工程技术研究中心，广东省广州市 510006；广东省生物医学工程重点实验室，广东省广州市 510006 weikun@scut.edu.cn
  • 作者简介:许为康★，男，1987年生，广东省揭阳市人，汉族，华南理工大学在读硕士，主要从事纳米生物材料研究。 xuweikang@ 126.com

Preparation and characterization of nano-silver slow-release chitosan/polyvinyl alcohol sponge wound dressings

Xu Wei-kang1,2,3, Wei Kun1,2,3, Zeng Xiao-feng1,2,3, Ling You1,2,3, Zhang Jie1,2,3, Wei Xin-miao1,2,3   

  1. 1School of Material Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong Province, China
    2National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, Guangdong Province , China
    3Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province ,China
  • Received:2012-01-19 Revised:2012-03-12 Online:2012-10-21 Published:2013-04-08
  • Contact: Wei Kun Doctor, Professor, School of Material Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong Province, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, Guangdong Province, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province ,China weikun@scut.edu.cn
  • About author:Xu Wei-kang★, Studying for master’s degree, School of Material Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong Province, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, Guangdong Province, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510006, Guangdong Province, China xuweikang@126.com

PDF

664

被引次数

9

摘要：

背景：临床上传统治疗创面感染主要使用抗生素药物敷料，但长期使用会诱导细菌耐药；同时纳米创伤敷料生物相容性差，无法降解，不宜长期覆盖创面。
目的：制备一种具有抗菌作用、生物相容性良好的伤口敷料，并表征分析其生物学性能。
方法：采用缩醛化反应制得含纳米银/聚乙二醇的壳聚糖/聚乙醇酸海绵，检测材料的物理性能、表面形貌、体外释放及抗菌性能。
结果与结论：制得的纳米银粒径小，分散性良好，体外释放实验表明纳米银粒子可持续不断地从辅料释放出来，作用于细菌。含纳米银/聚乙二醇的壳聚糖/聚乙烯醇海绵孔隙致密均匀，大小孔相互贯通；吸水性大、保湿性高、透气性适中；吸水率和透气率都随聚乙烯醇1799含量增加而增大；保湿率基本不变；对金黄色葡萄杆菌、大肠杆菌、白色念珠菌、铜绿假单胞菌、变伤寒沙门菌5种实验菌种均有良好杀菌效果。表明该敷料物理性能好，生物相容性及杀菌效果好。

关键词: 纳米银, 聚乙二醇, 聚乙烯醇, 壳聚糖, 伤口敷料, 生物材料

Abstract:

BACKGROUND: The clinical treatment of traditional wound infection is mainly use antibiotic drugs dressings, but the long-term use can cause bacteria resistance. Meanwhile, nano wound dressings used in clinical application has poor biocompatibility and cannot be degraded, which should not be used for long-term coverage of wound.
OBJECTIVE: To prepare a wound dressing with antibacterial action and good biocompatibility, and to characterize and analysis its biological properties.
METHODS: Chitosan/polyvinyl alcohol sponge containing nano-silver/polyethyleneglycol was prepared by acetalization reaction. The material’s mechanical properties, surface topography, in vitro release, and antibacterial properties were detected.
RESULTS AND CONCLUSION: The particle diameter of nano-silver prepared was small and it had good disparity. The in vitro release studies showed that Ag nano-silver particles were able to release continuously and sterilize the bacterium. The Chitosan/polyvinyl alcohol sponge containing nano-silver/polyethyleneglycol had dense and uniform interspaces; the pores inside was interconnected, and had favorable moisture-adsorption rate, moisture-retention rate, and moderate air permeability rate. The moisture-adsorption rate and air permeability rate were increased with the content of polyvinyl alcohol 1799 increased, but the moisture-retention rate almost had no effects with the changes of the content of polyvinyl alcohol 1799. The material had a good bactericidal effect on the staphylococcus aureus, escherichia coli, candida albicans, pseudomonas aeruginosa and salmonella typhi. These findings suggest that the dressing not only possesses good physical properties and anti-microbial, but also its synthesis procedure is simple and low cost. Therefore, the developed material can be applied to new wound dressing.

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