中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (45): 8361-8366.doi: 10.3969/j.issn.2095-4344.2012.45.001

• 骨髓干细胞 bone marrow stem cells •    下一篇

骨髓间充质干细胞成骨分化过程中瞬时性受体电位香草精受体5的表达

李福春1，马学玲2，屈艳萍3，谷贵山4   

  1. 1哈尔滨医科大学附属第一医院骨一科，黑龙江省哈尔滨市 150001
    2哈尔滨医科大学附属第四医院神经内科，黑龙江省哈尔滨市 150001
    3黑龙江省医院门诊部，黑龙江省哈尔滨市 150001
    4吉林大学第一临床医学院骨关节一科，吉林省长春市 130021
  • 收稿日期:2012-08-09 修回日期:2012-09-03 出版日期:2012-11-04 发布日期:2013-03-16
  • 通讯作者: 谷贵山，博士生导师，教授，吉林大学第一医院骨关节一科，吉林省长春市 130021 guguishan@sina.com
  • 作者简介:李福春☆，男，1971年生，黑龙江省齐齐哈尔市人，朝鲜族，2008年吉林大学第一临床医学院毕业，博士，主治医师，主要从事复杂创伤及骨肉瘤发病机制的研究。 lfcmxl@163.com

Expression of transient receptor potential vanilloid receptor 5 during osteogenic differentiation of bone marrow mesenchymal stem cells

Li Fu-chun1, Ma Xue-ling2, Qu Yan-ping3, Gu Gui-shan4   

  1. 1First Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
    2Department of Neurology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
    3Department of Out-patient, Heilongjiang Provincial Hospital, Harbin 150001, Heilongjiang Province, China
    4First Department of Orthopedics, the First Clinical Medical College of Jilin University, Changchun 130021, Jilin Province, China
  • Received:2012-08-09 Revised:2012-09-03 Online:2012-11-04 Published:2013-03-16
  • Contact: Gu Gui-shan, Doctoral supervisor, Professor, First Department of Orthopedics, the First Clinical Medical College of Jilin University, Changchun 130021, Jilin Province, China guguishan@sina.com
  • About author:Li Fu-chun☆, Doctor, Attending physician, First Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China lfcmxl@163.com

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摘要：

背景：目前，钙离子通道的研究仅限于肾脏、小肠、光滑卵母细胞中瞬时性受体电位香草精受体5所表现出来的电生理特性。
目的：观察新型钙离子通道瞬时性受体电位香草精受体5在大鼠骨髓间充质干细胞来源的成骨细胞中的表达及意义。
方法：分离、提取、培养大鼠骨髓间充质干细胞，诱导大鼠骨髓间充质干细胞向成骨细胞分化，用免疫组织化学法检测大鼠骨髓间充质干细胞诱导分化的成骨细胞中瞬时性受体电位香草精受体5的表达。
结果与结论：实验成功将体外培养大鼠骨髓间充质干细胞诱导培养为成骨细胞。碱性磷酸酶染色和茜素红染色检测结果显示，诱导分化的成骨细胞中胞浆中可见紫黑色和红色的矿化结节。免疫组织化学染色结果显示，诱导的成骨细胞中瞬时性受体电位香草精受体5呈强阳性表达。结果证实，大鼠骨髓间充质干细胞来源的成骨细胞中存在瞬时性受体电位香草精受体5钙离子通道，此通道可能成为调节成骨细胞增殖和分化的候选通道。

关键词: 瞬时性受体电位通道, 骨髓间充质干细胞, 成骨细胞, 大鼠, 分化, 离子通道, 钙离子, 免疫组织化学, 骨髓来源干细胞, 组织工程

Abstract:

BACKGROUND: At present, the calcium ion channel research is confined in the electrophysiological properties demonstrated by transient receptor potential vanilla receptor 5 in the kidney, small intestine and smooth oocytes.
OBJECTIVE: To explore the expression and significance of new calcium ion channel transient receptor potential vanilla receptor 5 in rat bone marrow mesenchymal stem cells derived osteoblasts.
METHODS: The rat bone marrow mesenchymal stem cells were separated, extracted and cultivated, then the rat bone marrow mesenchymal stem cells were induced to differentiate into osteoblasts, the expression of transient receptor potential vanilla receptor 5 in osteoblasts derived from rat bone marrow mesenchymal stem cells was detected by immunohistochemistry.
RESULTS AND CONCLUSION: The in vitro cultured rat bone marrow mesenchymal stem cells were successfully induced to differentiate into osteoblasts. Alkaline phosphatase staining and alizarin red staining results showed that the purple-black and red mineralized nodules were visible in the cytoplasm of the osteoblasts derived from rat bone marrow mesenchymal stem cells. Immunohistochemical staining results showed the strongly positive expression of transient receptor potential vanilla receptor 5 in bone marrow mesenchymal stem cells derived osteoblasts. Results confirm that transient receptor potential vanilla receptor 5 calcium ion channel exists in rat bone marrow mesenchymal stem cells derived osteoblasts, and the transient receptor potential vanilla receptor 5 calcium ion channel may be the candidate channel which can regulate the osteoblasts proliferation and differentiation.

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