中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (47): 8807-8812.doi: 10.3969/j.issn.2095-4344.2012.47.015

• 复合支架材料 composite scaffold materials • 上一篇    下一篇

结核杆菌黏附不同人工关节假体材料的能力研究

马 骏，李国庆，曹 力   

  1. 新疆医科大学第一附属医院骨科，新疆维吾尔自治区乌鲁木齐市 830054
  • 收稿日期:2012-08-11 修回日期:2012-09-05 出版日期:2012-11-18 发布日期:2013-03-15
  • 作者简介:马骏★，男，1984年生，新疆维吾尔自治区乌鲁木齐市人，汉族，新疆医科大学在读硕士，主要从事骨与关节疾患研究。 mjzazj114@163.com

Adhesive ability of Mycobacterium tuberculosis onto the surface of different joint prosthesis materials

Ma Jun, Li Guo-qing, Cao Li   

  1. Department of Orthopedics, First Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2012-08-11 Revised:2012-09-05 Online:2012-11-18 Published:2013-03-15
  • About author:Ma Jun★, Studying for master’s degree, Department of Orthopedics, First Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China mjzazj114@163.com

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631

被引次数

13

摘要：

背景：细菌黏附在假体表面大量繁殖并形成致密的生物膜，药物难以进入将其杀灭是人工关节置换后感染难治及复发的主要原因。
目的：观察不同人工关节假体材料性质、表面对结核杆菌黏附能力的影响。
方法：取钛合金和钴铬钼合金试样(均分光滑面和粗糙面两种)，分别将结核杆菌与表皮葡萄球菌(对照菌液)经传代培养后由FITC标记，制成菌液；将菌液与试样37 ℃共同培养24 h；用荧光显微镜和电镜扫描观察两种细菌在上述4种不同试样的黏附情况。
结果与结论：表皮葡萄球菌和结核杆菌在钴铬钼合金表面的黏附面积均大于在钛合金表面的黏附面积；在同种材料中粗糙面比光滑面更容易发生细菌黏附；两种材料表面均可见表皮葡萄球菌形成生物膜，而结核杆菌则无生物膜形成。表明结核杆菌在钴铬钼合金、钛合金表面的黏附能力较差；材料性质和材料表面粗糙程度均对结核杆菌黏附力产生巨大影响；结核杆菌在钴铬钼合金和钛合金表面并无生物膜形成。

关键词: 人工关节假体, 钛合金, 钴铬钼合金, 关节结核, 感染, 结核杆菌, 表皮葡萄球菌, 细菌黏附, 生物材料

Abstract:

BACKGROUND: Bacteria adhere on the prosthesis surface and form a biological membrane, so the drugs cannot kill the bacteria. It is the main reason for refractory treatment and recurrence of artificial joint replacement infections.
OBJECTIVE: To investigate the influence of properties and surfaces of different artificial joint prosthesis materials on the adhesion abilities of Mycobacterium tuberculosis.
METHODS: Specimens of titanium alloy and cobalt chromium molybdenum alloy were made respectively (with smooth surface and rough surface), Mycobacterium tuberculosis was labeled by FITC after subculture in order to make into bacterial liquid; at the same time, the Staphylococcus epidermidis was collected for control study. Bacteria liquid and samples were co-cultured at 37 ℃ for 24 hours and then observed using fluorescence microscope and scanning electron microscope to identify the adhesion conditions of these two bacteria.
RESULTS AND CONCLUSION: The adhesion areas of Staphylococcus epidermidis and Mycobacterium tuberculosis on the cobalt chromium molybdenum alloy were larger than that on the titanium alloy; in the case of the same material, the rough surface was more prone to bacterial adhesion than the smooth surface; there was biofilm formation for Staphylococcus epidermidis on the surface of two kinds of materials, while there was no biofilm formation for Mycobacterium tuberculosis. This study suggests that the Mycobacterium tuberculosis has poor adhesion ability on the surface of cobalt chromium molybdenum alloy and titanium alloy; the property and the roughness of the material have tremendous effects on the adhesion abilities of Mycobacterium tuberculosis; and there is no biofilm formation for Mycobacterium tuberculosis on the surface of cobalt chromium molybdenum alloy and titanium alloy.

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