中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (36): 9381-9392.doi: 10.12307/2026.908

• 骨组织构建 bone tissue construction •    下一篇

杜仲苷促进小鼠颅顶前成骨细胞MC3T3-E1的成骨分化

王厚元1，2，肖嘉聪1，2，邵首嘉1，3，何拍岸1，3，杨  楠1，3，姜自伟1，2，3   

  1. 1广州中医药大学，广东省广州市  510405；2广州中医药大学第一临床医学院，广东省广州市  510405；3广州中医药大学第一附属医院，广东省广州市  510405
  • 收稿日期:2025-10-29 修回日期:2026-03-14 出版日期:2026-12-28 发布日期:2026-05-20
  • 通讯作者: 姜自伟，博士，主任医师，博士生导师，广州中医药大学，广东省广州市 510405；广州中医药大学第一临床医学院，广东省广州市 510405；广州中医药大学第一附属医院，广东省广州市 510405
  • 作者简介:王厚元，男，1995年生，广州中医药大学在读博士研究生，主要从事骨与关节疾病研究。
  • 基金资助:
    国家自然科学基金项目(81974575)，项目负责人：姜自伟

Aucubin promotes osteogenic differentiation of mouse cranial pre-osteoblasts MC3T3-E1

Wang Houyuan1, 2, Xiao Jiacong1, 2, Shao Shoujia1, 3, He Paian1, 3, Yang Nan1, 3, Jiang Ziwei1, 2, 3   

  1. ¹Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; 2The First Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; 3The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
  • Received:2025-10-29 Revised:2026-03-14 Online:2026-12-28 Published:2026-05-20
  • Contact: Jiang Ziwei, PhD, Chief physician, Doctoral supervisor, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; The First Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
  • About author:Wang Houyuan, PhD candidate, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China; The First Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China
  • Supported by:
    National Natural Science Foundation of China, No. 81974575 (to JZW)

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摘要：


文题释义：
杜仲苷：是杜仲的主要活性成分之一，具有抗氧化、抗炎、调节糖脂代谢、保护心血管、改善骨代谢等作用。
Janus激酶2/信号转导与转录激活因子3信号通路：是细胞信号传导中的重要通路，主要涉及细胞增殖、存活、免疫调节和抗氧化等功能。

背景：氧化应激是诱发骨质疏松等骨代谢疾病的关键因素之一，杜仲苷为天然活性物质，兼具抗氧化与促成骨作用，但作用机制尚未阐明。 
目的：借助网络药理学结合体外细胞实验，探究杜仲苷促成骨分化的潜在靶点及信号通路。
方法：通过TCMSP、GeneCards等数据库筛选杜仲苷潜在成骨分化相关靶点，构建蛋白-蛋白互作网络并进行富集分析预测靶点通路。应用过氧化氢建立MC3T3-E1细胞氧化应激模型，分为对照组、模型组、杜仲苷低、高剂量组以及杜仲苷联合通路抑制剂组，随后进行细胞活力、丙二醛水平检测以及碱性磷酸酶染色、茜素红染色；采用Western blot和RT-qPCR检测Janus激酶2/信号转导与转录激活因子3通路相关蛋白及成骨相关基因、蛋白表达。
结果与结论：①网络药理学结果显示杜仲苷可能通过Janus激酶2/信号转导与转录激活因子3通路参与骨代谢调控，核心靶点包括Janus激酶2、信号转导与转录激活因子3、Janus激酶1、白细胞介素2、基质金属蛋白酶9 等；②体外细胞实验结果显示，杜仲苷能够抑制氧化应激，降低丙二醛水平，促进成骨分化及矿化结节形成，同时上调Janus激酶2/信号转导与转录激活因子3通路蛋白的表达以及成骨标志物(Runt相关转录因子2、骨钙素、骨桥蛋白)基因和蛋白表达，且这种作用可被Janus激酶2/信号转导与转录激活因子3通路抑制剂AG490部分阻断。结果表明：杜仲苷可能通过激活Janus激酶2/信号转导与转录激活因子3信号通路，减轻氧化应激损伤，促进 MC3T3-E1细胞成骨分化。
https://orcid.org/0009-0007-5968-0388(王厚元)


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 杜仲苷, 氧化应激, 成骨分化, 骨质疏松, 网络药理学, Janus激酶2/信号转导与转录激活因子3

Abstract: BACKGROUND: Oxidative stress is a major contributor to osteoporosis and other bone metabolic disorders. Aucubin, a natural compound with antioxidant and osteogenic effects, shows therapeutic potential, but its mechanisms remain unclear.
OBJECTIVE: To investigate the potential targets and pathways through which aucubin promotes osteogenic differentiation using network pharmacology combined with in vitro validation.
METHODS: Potential aucubin targets related to osteogenesis were identified from TCMSP and GeneCards databases, and a protein-protein interaction network with enrichment analysis was constructed to predict target pathways. An oxidative stress model of MC3T3-E1 cells was established using hydrogen peroxide, and the cells were assigned to control, model, low- and high-dose aucubin intervention, and Aucubin+JAK2/STAT3 inhibitor groups. Cell viability and malondialdehyde level were detected, and alkaline phosphatase staining and alizarin red staining were performed. Western blot and RT-qPCR were used to detect the expression of Janus kinase 2/signal transducer and activator of transcription 3 pathway-related proteins and osteogenic proteins and genes.
RESULTS AND CONCLUSION: (1) Network pharmacology predicted that aucubin functioned mainly via the Janus kinase 2/signal transducer and activator of transcription 3 pathway, involving key targets such as Janus kinase 2, signal transducer and activator of transcription 3, Janus kinase 1, interleukin 2, and matrix metalloproteinase 9. (2) In vitro experiments demonstrated that aucubin reduced oxidative stress, decreased malondialdehyde levels, enhanced alkaline phosphatase activity and mineralization, and upregulated the expression of pathway proteins as well as the gene and protein expression of osteogenic markers (Runt-related transcription factor 2, osteocalcin, osteopontin). These effects were partially reversed by the pathway inhibitor AG490. Taken together, these findings indicate that aucubin alleviates oxidative stress and promotes osteogenic differentiation of MC3T3-E1 cells through activation of the Janus kinase 2/signal transducer and activator of transcription 3 pathway.

Key words: aucubin, oxidative stress, osteogenic differentiation, osteoporosis, network pharmacology, Janus kinase 2/signal transducer and activator of transcription 3

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