中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (22): 5749-5755.doi: 10.12307/2026.091

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

运动预处理中蛋白激酶C对模型大鼠心肌细胞SarcKATP通道表达的影响

王  凯1，周跃辉2   

  1. 1江苏师范大学体育学院，江苏省徐州市   221116；2曲阜师范大学体育科学学院，山东省曲阜市   273165
  • 收稿日期:2025-02-15 接受日期:2025-05-30 出版日期:2026-08-08 发布日期:2025-12-27
  • 通讯作者: 周跃辉，博士，副教授，曲阜师范大学体育科学学院，曲阜市山东省 273165
  • 作者简介:王凯，男，1976年生，山东省临沂市人，汉族，2012年上海体育学院毕业，博士，副教授，主要从事运动与心血管健康的研究。
  • 基金资助:
    江苏省教育科学“十四五”规划课题(T-b/2021/35)，项目负责人：王凯；江苏省社科基金(22TYB009)，项目负责人：王凯

Effects of protein kinase C on the expression of myocardial SarcKATP channels in model rats during exercise preconditioning

Wang Kai1, Zhou Yuehui2   

  1. 1School of Physical Education, Jiangsu Normal University, Xuzhou 221116, Jiangsu Province, China; 2School of Sports Science, Qufu Normal University, Qufu 273165, Shandong Province, China
  • Received:2025-02-15 Accepted:2025-05-30 Online:2026-08-08 Published:2025-12-27
  • Contact: Zhou Yuehui, PhD, Associate professor, School of Sports Science, Qufu Normal University, Qufu 273165, Shandong Province, China
  • About author:Wang Kai, PhD, Associate professor, School of Physical Education, Jiangsu Normal University, Xuzhou 221116, Jiangsu Province, China
  • Supported by:
    Jiangsu Province Education Science Project during the “14th Five-Year Plan,” No. T-b/2021/35 (to WK); Jiangsu Province Social Science Foundation, No. 22TYB009 (to WK) 

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摘要：


文题释义：
蛋白激酶C(protein kinase C，PKC)：是一种细胞质酶，在未受刺激的细胞中，蛋白激酶C主要分布在细胞质中，呈非活性构象。一旦有第二信使的存在，蛋白激酶C将成为膜结合的酶，它能激活细胞质中的酶，参与生化反应的调控，同时也能作用于细胞核中的转录因子，参与基因表达的调控，是一种多功能的酶。比较常见的有蛋白激酶C参与受体脱敏、调节膜结构、调节转录、介导免疫应答、调节细胞生长以及学习和记忆等，这些功能是通过蛋白激酶C介导的其他蛋白质的磷酸化来实现的。
心肌SarcKATP通道：ATP敏感性钾通道(ATP-sensitive potassium channel，KATP)于1983年由Noma首先在豚鼠的心肌细胞上发现，其特征是通道活性随胞内ATP浓度升高而被显著抑制。现已证明多种组织细胞包括人的心肌细胞存在KATP通道，尤其在心肌缺血、室性心动过速、心衰的情况下是重要的心脏保护因子，对于指导临床药物治疗、靶点的选择上具有重要的价值。

背景：运动预处理能够产生早、晚期心肌保护效应，而蛋白激酶C和心肌SarcKATP通道分别是该保护效应中的中介物质和效应物质。蛋白激酶C能调控心肌SarcKATP通道的表达。
目的：比较运动预处理早、晚期中蛋白激酶C对心肌SarcKATP通道亚基Kir6.2和SUR2A表达的影响。
方法：SD大鼠48只随机分为5组，分别是对照组(不做处理)、运动(预处理)早期组、白屈菜赤碱(蛋白激酶C抑制剂，运动前腹腔注射)+运动早期组、运动(预处理)晚期组和白屈菜赤碱+运动晚期组。预处理结束后，用实时荧光定量PCR检测大鼠心肌Kir6.2 和SUR2A 的mRNA表达及变化趋势，用免疫印迹方法检测Kir6.2和SUR2A蛋白表达及变化趋势。
结果与结论：①与对照组相比，运动早期组和运动晚期组Kir6.2和SUR2A mRNA表达差异无显著性意义。与运动早期组相比，白屈菜赤碱+运动早期组Kir6.2 mRNA表达下降，而Kir6.2蛋白表达水平升高；SUR2A mRNA和蛋白表达水平都下降。②与运动晚期组相比，白屈菜赤碱+运动晚期组Kir6.2 mRNA和SUR2A mRNA表达下降，Kir6.2蛋白表达降低，而SUR2A表达升高。③结果说明，对心肌Kir6.2或SUR2A同一亚基而言，蛋白激酶C在运动预处理早、晚期中对其调控的影响是协调互补的，而对于心肌Kir6.2和SUR2A不同亚基而言，蛋白激酶C在运动预处理早、晚期中对其表达调控的影响也是协调互补的。

https://orcid.org/0000-0002-6555-9548 (王凯) 


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 蛋白激酶C, 运动预处理, 心肌, ATP敏感钾通道, Kir6.2, SUR2A

Abstract: BACKGROUND: Exercise preconditioning produces early and late myocardial protective effects, in which protein kinase C and myocardial ATP-sensitive potassium channels (SarcKATP) are mediators and effectors, respectively. Protein kinase C regulates the expression of myocardial SarcKATP channels.
OBJECTIVE: To compare the effects of protein kinase C on the expression of myocardial SarcKATP channel subunits, inward recirculating potassium channel 6.2 (Kir6.2) and sulfonylurea receptor 2A (SUR2A), in exercise preconditioning.
METHODS: Forty-eight Sprague-Dawley rats were randomly divided into five groups: control group (no intervention), early exercise preconditioning group, protein kinase C inhibitor (pre-exercise intraperitoneal injection) + early exercise preconditioning group, late exercise preconditioning group, and protein kinase C inhibitor + late exercise preconditioning group. After preconditioning, the distribution and expression changes of Kir6.2 and SUR2A mRNAs in the rat myocardium were observed and detected using real-time fluorescent quantitative PCR. The distribution and expression changes of Kir6.2 and SUR2A proteins were observed and detected using western blot.
RESULTS AND CONCLUSION: (1) Compared with the control group, the mRNA expression of Kir6.2 and SUR2A showed no significant difference in the early and late exercise preconditioning groups. Compared with the early exercise preconditioning group, the protein kinase C inhibitor + early exercise preconditioning group showed a decrease in Kir6.2 mRNA expression and an increase in Kir6.2 protein expression, while both SUR2A mRNA and protein levels decreased. (2) Compared with the late exercise preconditioning group, the protein kinase C inhibitor + late exercise preconditioning group showed reduced Kir6.2 expression at mRNA and protein levels; meanwhile, SUR2A mRNA expression reduced and SUR2A expression increased. For the same subunit of myocardial Kir6.2 or SUR2A, protein kinase C exerts coordinated and complementary regulatory effects on its expression regulation in early and late exercise preconditioning, and as for different subunits of Kir6.2 and SUR2A, protein kinase C also has coordinated and complementary effects on their expression regulation in early and late exercise preconditioning.


Key words: protein kinase C, exercise preconditioning, myocardium, ATP-sensitive potassium channels, Kir6.2, SUR2A

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