中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (37): 5524-5529.doi: 10.3969/j.issn.2095-4344.2016.37.008

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

血竭对组织工程皮肤移植模型大鼠创伤修复的影响

俞  琦,王文佳,王  平   

  1. 贵阳中医学院基础医学院,贵州省贵阳市  550025
  • 出版日期:2016-09-09 发布日期:2016-09-09
  • 作者简介:俞琦,女,1978年生,贵州省贵阳市人,汉族,2001年河北医科大学毕业,硕士,副教授,主要从事中西医结合方面的研究。
  • 基金资助:

    贵州省科技厅-贵阳中医学院联合基金项目(黔科合中药字[2012]LKZ7029号

Effects of Sanguis draxonis on wound healing in rat models of tissue-engineered skin transplantation

Yu Qi, Wang Wen-jia, Wang Ping   

  1. School of Basic Medicine, Guiyang University of Chinese Medicine, Guiyang 550025, Guizhou Province, China
  • Online:2016-09-09 Published:2016-09-09
  • About author:Yu Qi, Master, Associate professor, School of Basic Medicine, Guiyang University of Chinese Medicine, Guiyang 550025, Guizhou Province, China
  • Supported by:

    the Project of Science and Technology Department of Guizhou Province-Guiyang University of Chinese Medicine, No. [2012]LKZ7029

摘要:

文章快速阅读:


文题释义:
血竭:又名麒麟竭,为棕榈科植物麒麟竭果实渗出的树脂经加工制成。具有活血定痛,止血,生肌敛疮的功效。主治跌打损伤、瘀血肿痛、金疮出血、溃疡不敛等。其药理活性表现为抗炎、止痛,以及活血与止血的双向调节作用等方面。
组织工程皮肤:是一种较为完善的组织工程化组织,是通过在体外大量培养和扩增功能细胞,将其与细胞外基质及支架材料互相作用,制成的具有生物活性的人工皮肤替代物。组织工程皮肤为皮肤创伤的修复、皮肤功能的重塑提供了新的策略。
摘要
背景:
研究显示,血竭治疗皮肤压疮、烧烫伤、溃疡等具有显著疗效。
目的:观察血竭对组织工程皮肤移植大鼠血管内皮细胞生长因子、表皮生长因子、神经肽P物质和羟脯氨酸的影响,验证血竭促进组织工程皮肤修复创面的作用。
方法:制备组织工程皮肤移植大鼠模型,将大鼠随机分为模型对照组、血竭外用组、血竭内服组、血竭外用加内服组,治疗组分别采用药物外敷、单纯口服[0.1 g/(kg•d)]、外敷加口服治疗,7 d后检测皮肤组织中血管内皮细胞生长因子、表皮生长因子、P物质和羟脯氨酸的表达。
结果与结论:①与模型对照组比较,血竭治疗组皮肤组织中血管内皮细胞生长因子、表皮生长因子和羟脯氨酸表达水平显著提高(P < 0.01或P < 0.05),P物质含量无改变(P > 0.05)。②结果说明,血竭可通过上调组织工程皮肤移植模型大鼠血管内皮细胞生长因子、表皮生长因子和羟脯氨酸的表达,促进组织工程皮肤对创面的修复作用。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0002-4723-3288(俞琦)

关键词: 组织构建, 组织工程, 血竭, 皮肤创伤, 创伤修复作用, 血管内皮细胞生长因子, 表皮生长因子, P物质, 羟脯氨酸

Abstract:

BACKGROUND: Increasing evidence suggests that Sanguis draxonis is of great significance for treating pressure sores, burns and ulcers.
OBJECTIVE: To observe the influences of Sanguis draxonis on vascular endothelial growth factor, epidermal growth factor, substance P and hydroxyproline in rats undergoing tissue-engineered skin transplantation and to verify its promotion of wound repair.
METHODS: The tissue-engineered skin transplantation model of rats were established. Rats in treatment groups were given external application, single oral use of 0.1 g/(kg•d) Sanguis draxonis and combined use, respectively. No treatment was given in control group. After continuous treatment for 7 days, the expression levels of vascular endothelial growth factor, epidermal growth factor, substance P and hydroxyproline in skin tissue were determined.
RESULTS AND CONCLUSION: Compared with the control group, the levels of vascular endothelial growth factor, epidermal growth factor, and hydroxyproline were significantly increased (P < 0.05 or P < 0.01), while substance P had no change in the treatment groups (P > 0.05). These results demonstrate that Sanguis draxonis can promote tissue-engineered skin to repair skin wound by upregulating the expression levels of vascular endothelial growth factor, epidermal growth factor, and hydroxyproline.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Drugs, Chinese Herbal, Skin, Transplantation

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