中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (2): 165-170.doi: 10.3969/j.issn.2095-4344.2017.02.001

• 组织工程口腔材料 tissue-engineered oral materials •    下一篇

生长因子复合支架材料在即刻种植周围骨缺损中的作用

杨玉鹏1，杨圣俊2，程凤峡1，谷建琦1，郑  瑶1，李  娟1，郝  玮1，吴永生1
  

  1. 河北省人民医院，1口腔科，2公费医保办，河北省石家庄市  050051
  • 收稿日期:2016-10-23 出版日期:2017-01-18 发布日期:2017-02-27
  • 通讯作者: 吴永生，硕士，教授，主任医师，硕士生导师，河北省人民医院口腔科，河北省石家庄市 050051
  • 作者简介:杨玉鹏，男，1983年生，河北省石家庄市人，汉族，2009年河北医科大学毕业，硕士，主治医师，主要从事口腔颌面外科。
  • 基金资助:

    2014年度河北省医学科学研究课题计划(ZL20140216)

Growth factor composite scaffolds for bone defect repair via immediate implantation of bone defects

Yang Yu-peng1, Yang Sheng-jun2, Cheng Feng-xia1, Gu Jian-qi1, Zheng Yao1, Li Juan1, Hao Wei1, Wu Yong-sheng1 
  

  1. 1Department of Stomatology, 2Medical Insurance Office, Hebei General Hospital, Shijiazhuang 050051, Heibei Province, China
  • Received:2016-10-23 Online:2017-01-18 Published:2017-02-27
  • Contact: Wu Yong-sheng, Master, Professor, Chief physician, Master’s supervisor, Department of Stomatology, Hebei General Hospital, Shijiazhuang 050051, Heibei Province, China
  • About author:Yang Yu-peng, Master, Attending physician, Department of Stomatology, Hebei General Hospital, Shijiazhuang 050051, Heibei Province, China
  • Supported by:

    the Medical Science Research Project of Hebei Province in 2014, No. ZL20140216

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摘要：

文章快速阅读：

 

文题释义：
即刻种植：指的是在拔牙后，即刻植入人工种植体。关于拔牙后种植体植入的时机，目前共识性的分类分为4类，即刻种植为第一类，其定义为拔牙后即刻植入种植体；其中第二类和第三类可归于拔牙后早期种植(early implant placement)；第四类种植时机亦称为后期种植(late implant placement)。
锶磷灰石：是一种新型的磷灰石类陶瓷，由锶(Sr)置换羟磷灰石[Ca10(PO4 )6(OH)2 ]中的部分钙(Ca)而制成。作为替代人体硬组织的生物材料，许多学者对其进行了各种体外测试，证实比传统的羟磷灰石有较大的优越性。

背景：研究发现神经生长因子在骨的愈合过程中发挥重要作用，但关于生长因子复合支架材料在犬即刻种植周围骨缺损中作用的研究不多。
目的：分析神经生长因子复合支架材料在犬即刻种植周围骨缺损中的作用。
方法：制备神经生长因子复合锶磷灰石支架材料，建立犬下颌骨缺损动物模型，将犬下颌骨缺损种植体分为实验组、阳性对照组和空白对照组，实验组植入神经生长因子复合锶磷灰石；阳性对照组植入锶磷灰石；空白对照组不植入任何材料。观察3组犬下颌骨种植体周围骨缺损的CT三维图像重建情况和苏木精-伊红染色情况。
结果与结论：①空白对照组犬骨缺损中央没有新骨生成，周围有少量新生骨生成；阳性对照组和实验组犬骨缺损处骨小梁从中间向四周扩散形成骨修复；②实验组犬下颌骨种植体周围骨小梁的骨密度、数量、厚度、种植体-骨结合率均高于阳性对照组和空白对照组(P < 0.05)，阳性对照组4项指标高于空白对照组(P < 0.05)；实验组犬下颌骨种植体周围骨小梁分离度低于阳性对照组和空白对照组(P < 0.05)，阳性对照组低于空白对照组(P < 0.05)；③苏木精-伊红染色显示，实验组犬下颌骨种植体支架网孔中有大量新骨生成，骨小梁排列整齐，周围骨质和新生组织连接紧密；阳性对照组犬下颌骨可见新生类骨质和骨小梁，以及少量死骨碎片；空白对照组骨缺损处有部分新骨生成，骨小梁排列不整齐，周围骨质和新生组织连接不紧密；④结果表明，神经生长因子复合支架材料能够促进犬即刻种植周围骨缺损的骨生成，是一种比较好的骨修复材料。

关键词: 生物材料, 口腔生物材料, 神经生长因子, 锶磷灰石, 支架材料, 犬, 即刻种植, 骨缺损, 骨生成

Abstract:

BACKGROUND: Nerve growth factor has been shown to play an important role in bone healing, but little is reported on the effect of growth factor composite scaffolds via the immediate implantation in the repair of canine bone defects.
OBJECTIVE: To analyze the effect of nerve growth factor composite scaffolds via the immediate implantation for the repair of canine bone defects.
METHODS: Nerve growth factor composited strontium apatite scaffolds were prepared. Canine mandibular defect models were established and divided into three groups, followed by implanted with composite scaffold (experimental group), strontium apatite (positive control group), or nothing (blank control group). The three-dimensional CT reconstruction and hematoxylin-eosin staining of canine mandibular bone defects were observed.
RESULTS AND CONCLUSION: In the blank control group, there were few new bones surrounding bone defect. Trabecular bones spread from the defect center to the surrounding tissues in the experimental and positive control groups. The bone density, volume, thickness, and implant-bone contact were significantly increased, while the trabecular separation was significantly decreased in the experimental group compared with the positive control and blank control groups (P < 0.05), and all above indicators in the positive contro group were significantly higher than those in the blank control group (P < 0.05). Hematoxylin-eosin staining showed that in the experimental group, there were a large number of new bones that contacted with the surrounding bones closely, and trabecular bones arranged regularly. In the positive control group, newborn osteoid, trabeculare, and a small amount of debris were found. In the blank control group, few new bones were connected with the surrounding bones untightly and trabecular bone arranged irregularly. These results indicate that the nerve growth factor composite scaffold can promote the bone regeneration in the canine bone defects after immediate implantation. 

Key words: Nerve Growth Factor, Stents, Biocompatible Materials, Tissue Engineering

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