中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (12): 1852-1857.doi: 10.3969/j.issn.2095-4344.2015.12.010

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米羟基磷灰石薄膜与人脐静脉血管内皮细胞的增殖

程光存，严中亚，李春生，严  宇，韦晓勇   

  1. 安徽医科大学附属省立医院胸心外科，安徽省心血管病研究所，安徽省合肥市  230001
  • 修回日期:2015-03-01 出版日期:2015-03-19 发布日期:2015-03-19
  • 通讯作者: 严中亚，教授，主任医师，安徽医科大学附属省立医院胸心外科，安徽省心血管病研究所，安徽省合肥市 230001
  • 作者简介:程光存，男，1966年生，安徽省舒城市人，硕士，副主任医师，副教授，主要从事体外循环在在心脏外科学中的应用研究。
  • 基金资助:

    安徽省自然科学基金项目(1308085MH143)

Nano-hydroxyapatite film as a support to improve the proliferation of human umbilical vein endothelial cells

Cheng Guang-cun, Yan Zhong-ya, Li Chun-sheng, Yan Yu, Wei Xiao-yong   

  1. Department of Cardiothoracic Surgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China
  • Revised:2015-03-01 Online:2015-03-19 Published:2015-03-19
  • Contact: Yan Zhong-ya, Professor, Chief physician, Department of Cardiothoracic Surgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China
  • About author:Cheng Guang-cun, Master, Associate chief physician, Associate professor, Department of Cardiothoracic Surgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China
  • Supported by:

    the Natural Science Foundation of Anhui Province, No. 1308085MH143

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摘要：

背景：合肥大学材料系和中国科学院安徽光学密精机械研究所联合研究应用脉冲激光沉积合成技术制备出一种新型的纳米羟基磷灰石人工心脏机械瓣膜。
目的：观察纳米羟基磷灰石人工心脏机械瓣膜与人脐静脉血管内皮细胞的相容性。
方法：将体外分离培养的传2-4代人脐静脉血管内皮细胞悬液接种于纳米羟基磷灰石人工心脏机械瓣膜上，培养3，7，21 d后，扫描电子显微镜下观察细胞在纳米羟基磷灰石人工心脏机械瓣膜上的生长情况。分别采用纳米羟基磷灰石人工心脏机械瓣膜常温浸提液、纳米羟基磷灰石人工心脏机械瓣膜高温浸提液、高密度聚乙烯浸提液及苯酚溶液培养人脐静脉血管内皮细胞，72 h后采用MTT法检测细胞增殖情况。
结果与结论：扫描电镜观察培养3 d时，人脐静脉血管内皮细胞呈梭形或多边形，伸出突起黏附于纳米羟基磷灰石人工心脏机械瓣膜上；7 d时，可见瓣膜表面细胞伸展充分，连接融合；21 d时，细胞成片融合，牢固覆盖于瓣膜表面，部分区域形成细胞外基质。MTT检测结果显示，纳米羟基磷灰石人工心脏机械瓣膜对人脐静脉血管内皮细胞无细胞毒性，具有良好的细胞相容性。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 材料相容性, 羟基磷灰石薄膜, 人脐静脉血管内皮细胞, 脉冲激光沉积, 组织工程, 四唑盐比色法, 安徽省自然科学基金

Abstract:

BACKGROUND: A new type of nano-hydroxyapatite artificial mechanical heart valve has been developed using pulsed laser deposition technology at the Department of Materials, Hefei University and Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, China. 
OBJECTIVE: To investigate the compatibility of nano-hydroxyapatite artificial mechanical heart valve with human umbilical vein endothelial cells.
METHODS: Human umbilical vein endothelial cells were in vitro isolated, cultured and passaged to the 2-4 generations, and then the cell suspension was inoculated onto the nano-hydroxyapatite artificial mechanical heart valve. After 3, 7, 12 days of culture, the cell growth on the artificial mechanical heart valve was observed under scanning electron microscope. In addition, the human umbilical vein endothelial cells were respectively cultured in room-temperature and high-temperature extract liquids of nano-hydroxyapatite artificial mechanical heart valve, high-density polyethylene and phenol solution extracts for 72 hours, and then, the proliferation of cells was detected by MTT method.
RESULTS AND CONCLUSION: Under the scanning electron microscope, the human umbilical vein endothelial cells were fusiform- or polygon-shaped with protuberances adhered to the artificial mechanical heart value at 3 days of culture; the cells were stretched thoroughly and fused at 7 days of culture; and the cells were confluent to pieces that tightly overlaid the heart valve surface and the extracellular matrix was formed locally at 21 days of culture. Results from MTT test displayed that the nano-hydroxyapatite artificial mechanical heart valve had no cytotoxicity to the human umbilical vein endothelial cells, indicating a good cytocompatibility.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: Durapatite, Endothelial Cells, Umbilical Veins

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