中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8689-8692.doi: 10.3969/j.issn.2095-4344.2012.46.028

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

链球菌溶血素O提高细胞的通透性

唐新杰，盛玲玲，张 群，谢 峰   

  1. 上海交通大学医学院附属第九人民医院, 上海市 200011
  • 收稿日期:2012-08-17 修回日期:2012-10-15 出版日期:2012-11-11 发布日期:2013-03-16
  • 通讯作者: 谢峰，博士，主治医师，上海交通大学医学院附属第九人民医院,上海市 200011 xiefenghe@163.com
  • 作者简介:唐新杰★，女，1976年生，河南省洛阳市人，汉族，2012年上海交通大学医学院毕业，硕士，主要从事细胞抽提物重编程方面的研究。 Tangxinjie0916@163.com

Effects of streptolysin O on increasing cell permeability

Tang Xin-jie, Sheng Ling-ling, Zhang Qun, Xie Feng   

  1. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Medical College of Shanghai Jiao Tong University, Shanghai 200011, China
  • Received:2012-08-17 Revised:2012-10-15 Online:2012-11-11 Published:2013-03-16
  • Contact: Xie Feng, Doctor, Attending physician, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Medical College of Shanghai Jiao Tong University, Shanghai 200011, China xiefenghe@163.com
  • About author:Tang Xin-jie★, Master, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Medical College of Shanghai Jiao Tong University, Shanghai 200011, China tangxinjie0916@163.com

PDF

611

被引次数

4

摘要：

背景：实验发现链球菌溶血素O引起的细胞通透在一定程度上是可逆的，链球菌溶血素O产生的孔道在Ca2+存在的情况下可以自然封闭。
目的：观察链球菌溶血素O是否可以使猪成纤维细胞的通透性发生改变，及不同质量浓度的链球菌溶血素O对细胞状态的影响。
方法：分别使用质量浓度为0，230，300，400 μg/L的链球菌溶血素O溶液处理猪成纤维细胞，孵育50 min后，加入PI染液，观察细胞的通透率。加入含Ca2+的培养液封闭细胞后，分别观察细胞的形态变化及增殖能力的变化。
结果与结论：随着链球菌溶血素O溶液质量浓度的增加，细胞的通透率只轻度提高。链球菌溶血素O处理细胞后，细胞的形态没有发生明显的改变。质量浓度为230 μg/L的链球菌溶血素O溶液对细胞的增殖能力无明显影响，而300 μg/L和400 μg/L的链球菌溶血素O溶液明显地降低了细胞的增殖能力。提示质量浓度为230 μg/L的链球菌溶血素O溶液可以提高细胞的通透性，并保持细胞正常的形态及增殖能力。

关键词: 链球菌溶血素O, 通透性, 成纤维细胞, 细胞形态, 增殖能力, 钙离子, 蛋白质, 渗透屏障, 渗透率, 细胞培养

Abstract:

BACKGROUND: Streptolysin O (SLO)-induced cell permeability is reversible to a certain extent, and the pores can be closed in the medium containing Ca2+.
OBJECTIVE: To observe whether SLO could improve the permeability of pig fibroblasts and to observe the states of cells treated with SLO of different concentrations.
METHODS: Pig fibroblasts were permeated with SLO solution of different concentrations (0, 230, 300 and 400 μg/L respectively). After incubation of 50 minutes, the cells were stained with PI to observe cell permeability. When adding the medium containing Ca2+, the cells were plated. Morphology and multiplication capacity of these cells were detected.
RESULTS AND CONCLUSION: Along with increase of SLO solution concentration, the cell permeability improved slightly. When cells were treated with SLO, the cell morphology was not changed. 230 μg/L SLO had no influence on the capacity of cell proliferation, and however, 300 μg/L and 400 μg/L SLO reduced the proliferation capacity significantly appearing statistically difference when compared with 0 μg/L group. These findings indicate that 230 μg/L SLO could increase cell permeability with keeping the normal shape and proliferation ability of cells.

中图分类号: