中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (45): 8486-8490.doi: 10.3969/j.issn.2095-4344.2012.45.023

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

仙灵骨葆对骨质疏松大鼠骨量及骨髓基质干细胞成骨分化的影响

邢 磊1，焦颖华2，田发明3   

  1. 河北联合大学附属医院，1老年病科，2中西医结合科，河北省唐山市0630003河北联合大学医学实验研究中心，河北省唐山市 063000
  • 收稿日期:2012-01-31 修回日期:2012-04-01 出版日期:2012-11-04 发布日期:2013-03-16
  • 通讯作者: 田发明，博士，讲师，河北联合大学医学实验研究中心，河北省唐山市 063000 tfm9911316@163.com
  • 作者简介:邢磊★，男，1978年生，河北省霸州市人，汉族，2003年华北煤炭医学院毕业，硕士，主治医师，主要从事骨质疏松、骨性关节炎等中老年多发疾病的基础与临床研究。 xlpangdun001@163.com

Xianlinggubao affects bone mass and osteogenic differentiation of bone marrow stromal cells in rats

Xing Lei1, Jiao Ying-hua2, Tian Fa-ming3   

  1. 1Department of Gerontology, 2Department of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China; 3Medical Research Center, Hebei United University, Tangshan 063000, Hebei Province, China
  • Received:2012-01-31 Revised:2012-04-01 Online:2012-11-04 Published:2013-03-16
  • Contact: Tian Fa-ming, M.D., Lecturer, Medical Research Center, Hebei United University, Tangshan 063000, Hebei Province, China tfm9911316@163.com
  • About author:Xing Lei★, Master, Attending physician, Department of Gerontology, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China xlpangdun001@163.com

PDF

636

被引次数

29

摘要：

背景：双侧卵巢切除可造成大鼠骨量丢失，仙灵骨葆作为传统中药具有一定的促进骨形成作用。
目的：观察仙灵骨葆体内给药对骨质疏松大鼠骨量及骨髓基质干细胞成骨分化能力以及相关因子表达的影响。
方法：3月龄雌性SD大鼠24只随机数字表法均分为3组，卵巢切除组和仙灵骨葆组行卵巢切除，造模6周后仙灵骨葆组给予仙灵骨葆250 mg/(kg•d)，干预8周；正常对照组不予干预。
结果与结论：卵巢切除组L2-L4椎体骨密度显著低于其他两组，仙灵骨葆组仍显著低于正常对照组(P < 0.05)；卵巢切除组血清骨钙素、骨髓基质干细胞的骨形态发生蛋白2、骨钙素mRNA水平均低于其他两组(P < 0.05)。细胞外基质矿化能力亦明显低于正常对照组和仙灵骨葆组。提示大鼠去势14周后骨量丢失显著，仙灵骨葆可部分阻止其骨量丢失，其作用机制可能与促进大鼠骨髓基质干细胞的成骨分化有关。

关键词: 仙灵骨葆, 骨质疏松, 大鼠\骨密度, 骨髓基质干细胞, 干细胞, 骨钙素, 骨形态发生蛋白

Abstract:

BACKGROUND: Bone loss in rats can be achieved by bilateral ovariectomy. Xianlinggubao as a Chinese agent has been shown to promote bone formation.
OBJECTIVE: To investigate the effects of Xianlinggubao on bone mass and the expression level of genes related to osteogenic differentiation of bone marrow stromal cells in rats.
METHODS: Twenty-four 3-month-old Sprague-Dawley rats were randomly divided into three groups, with eight rats in each group. Rats from the ovariectomy group and Xianlinggubao group underwent ovariectomy. At 6 weeks after ovariectomy, Xianlinggubao group rats were daily administered 250 mg/kg Xianlinggubao for a total of 8 weeks. Rats from the normal control group received no intervention.
RESULTS AND CONCLUSION: The bone mineral density at L2-4 vertebral body in the Xianlinggubao group was significantly higher than that in the ovariectomy group, but it was significantly lower than that in the normal control group (both P < 0.05). Serum osteocalcin, bone morphogenetic protein 2, osteocalcin mRNA expression levels in the ovariectomy group were significantly lower than those in the Xianlinggubao group and normal control group (both P < 0.05). Extracellular matrix mineralization ability in the ovariectomy group was obviously lower than that in the Xianlinggubao group and normal control group. At 14 weeks after ovariectomy, rat bone loss was obvious, and Xianlinggubao can partly prevent bone loss. The underlying mechanism is likely to be related to Xianlinggubao promotion of osteogenic differentiation of rat bone marrow stromal cells.

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