关键词: 绿色荧光蛋白, 转基因鼠, C57BL雌鼠, 移植, 细胞移植, 骨髓细胞, 造血功能, 造血重建, 荧光原位杂交, 肝细胞, 白细胞, 脾细胞

Abstract:

BACKGROUND: It is reported that muscle-derived cells can rebuild the hematopoietic function, whether the other cells can rebuild the hematopoietic function also needs further observation.
OBJECTIVE: To compare the effect of different cells on the recovery of hematopoietic function of the C57BL female mice.
METHODS: We removed the eyeballs of green fluorescent protein transgenic male mice in order to collect the peripheral blood. Then the bloods were hemolyzed to prepare white blood cells. And then, the spleen and the liver were obtained to prepare the spleen cells and liver cells suspension. The bone marrow cells were prepared. The C57BLfemale mice were irradiated by half-lethal dose and transfused with four kinds of cells. At 18, 39 and 53 days after transplantation, green fluorescence protein-positive cells in peripheral blood were detected. At the same time, the bloods were marked with CD4-PE and CD8-PE. At 100 days after transplantation, mice were sacrificed and their bone marrows were collected to detect Y chromosome-positive rate.
RESULTS AND CONCLUSION: At 18, 39 and 53 days after transplantation, green fluorescence protein-positive cells were detected in peripheral blood of C57BL female mice; the percentage of green fluorescence protein-positive cells in the peripheral blood of C57BL female mice: transfused with spleen cells and marrow cells > transfused with liver cells > transfused with peripheral white blood cells. At the same time, the CD4 and green fluorescence protein double positive cells and CD8 and green fluorescence protein double positive cells were also detected in the peripheral blood of C57BL female mice after transfused with spleen cells and marrow cells, while the double positive cells were not detected in the peripheral blood of C57BL female mice after transfused with liver cells and peripheral white blood cells. At 100 days after transplantation, the Y chromosome-positive rate in bone marrow was positively correlated with the green fluorescence protein-positive rate in peripheral blood. The white blood cells, spleen cells, liver cells and effect marrow cells in the green fluorescent protein transgenic male mice can rebuild hematopoietic function, and the effect of spleen cells is similar to bone marrow cells but stronger than liver cells. The effects of liver cells are stronger than the white blood cells.