关键词: 小肠黏膜下层, 骨髓间充质干细胞, 复合培养, 组织工程, 神经, 标记, 生物材料, 生物相容性

Abstract:

BACKGROUND: Small intestinal submucosa extracellular matrix is a kind of material used to build the single structural engineering scaffolds with low immune response and good biocompatibility.
OBJECTIVE: To observe the biocompatibility of bone marrow mesenchymal stem cells growing on the small intestinal submucosa in vitro.
METHODS: The primary bone marrow mesenchymal stem cells were separated and purified by density gradient centrifugation combined with adherent methods. The cells were labeled using the red fluorescent cell linker kit before seeding. The passage 2 labeled bone marrow mesenchymal stem cells were seeded on the small intestinal submucosa.
RESULTS AND CONCLUSION: ①Histological observation showed that the bone marrow mesenchymal stem cells formed a single layer on the surface of small intestinal submucosa after coculture for 1 week. The labeled bone marrow mesenchymal stem cells on the scaffold showed uniformly red fluorescence by fluorescence microscope. Two weeks later, the cells were in multi-layer, and displayed intensively red fluorescence. ②Scanning electron microscope showed that, after 2 days of coculture, bone marrow mesenchymal stem cells were adherent to the small intestinal submucosa; 1 week later, the small intestinal submucosa was covered with the collagen secreted by bone marrow mesenchymal stem cells; 2 weeks later, the cells proliferated and fused on co-cultured small intestinal submucosa and closely connected, which showed stratifications and secreted a large number of matrix. The data obtained from the present study shows that bone marrow msenchymal stem cells and small intestinal submucosa have a good compatibility.