中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (33): 6189-6193.doi: 10.3969/j.issn.2095-4344.2012.33.022

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

体外贴壁和微囊化生长睾丸支持细胞形态与其功能的相关性

张国境1，赵 佳2，金 洁1，路 欣2，史小林2   

  1. 1首都医科大学燕京医学院组织胚胎学教研室，北京市 101300；
    2首都医科大学基础医学院组织胚胎学教研室，北京市 100069
  • 收稿日期:2012-01-10 修回日期:2012-01-10 出版日期:2012-08-12 发布日期:2013-07-15
  • 通讯作者: 史小林，教授，博士生导师，首都医科大学基础医学院组织胚胎学教研室，北京市 100069 shixl.good@ 163.com
  • 作者简介:张国境★，女，1967年生，北京市人，汉族，2005年首都医科大学毕业，硕士，副教授，主要从事生殖医学的研究。 zgj29@163.com

Correlation between the morphologies and physiological function of adherent and microencapsulated Sertoli cells

Zhang Guo-jing1, Zhao Jia2, Jin Jie1, Lu Xin2, Shi Xiao-lin2   

  1. 1Department of Histology and Embryology, Yanjing Medical College, Capital Medical University, Beijing 101300, China;
    2Department of Histology and Embryology, Basic Medical Hospital of Capital Medical University, Beijing 101300, China
  • Received:2012-01-10 Revised:2012-01-10 Online:2012-08-12 Published:2013-07-15
  • Contact: Shi Xiao-lin, Professor, Doctoral supervisor, Department of Histology and Embryology, Basic Medical Hospital of Capital Medical University, Beijing 1000069, China shixl.good@163.com
  • About author:Zhang Guo-jing★, Master, Assoicate professor, Department of Histology and Embryology, Yanjing Medical College, Capital Medical University, Beijing 1000069, China zgj29@163.com

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摘要：

背景：细胞体外培养一直是研究活细胞的主要方法，但体外培养细胞的形态和生长环境与体内环境有很大差异，因此，细胞的生物活性是否能准确反映体内该细胞的状态尚不清楚。
目的：观察体外培养细胞的形态与其生理功能间的相关性。
方法：体外培养不同生长状态的小鼠睾丸支持细胞，一种为贴壁生长状态，细胞呈扁平状；另一种为微囊化生长状态，细胞呈立体球状。分别取2种不同形态睾丸支持细胞的培养液，进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，获取相应的蛋白质条带，通过蛋白质相对分子质量的计算来确认睾丸支持细胞分泌蛋白质的种类。
结果与结论：贴壁生长的扁平状的睾丸支持细胞培养液中可辨别出14个条带，蛋白质相对分子质量分布在(17~158)×103之间；微囊化生长的立体球状睾丸支持细胞培养液中可辨别出10个条带，蛋白质相对分子质量分布在(17~58)×103之间。提示不同形态的睾丸支持细胞在蛋白质分泌数量和种类上都存在着差异，细胞的形态与其生理功能之间存在着明显的相关性。

关键词: 睾丸支持细胞, 条件培养液, 体外培养, 贴壁, 微囊, 形态

Abstract:

BACKGROUND: In vitro culture has been a main method for studying the living cells. However, there is a big difference in the morphology of the cells and growth environment between in vitro and in vivo. So it is unclear whether the bioactivity of cells in vitro can reflect precisely the cell growth in vivo.
OBJECTIVE: To investigate the correlation between the morphologies of the cells cultured in vitro and their physiological function.
METHODS: Different morphologies of mouce Sertoli cells were cultured in vitro. One of morphologies was the growth of cell adherence, and the cells were flat; another cells grew in microcapsule, which showed sphere. Culture solution from the two kinds of morphologies of SCs was taken out, and sodium dodecyl sulfate polyacrylamide gel electrophoresis was finished for obtaining the corresponding electrophoretic band. And then, the molecular weight of protein was calculated to ascertain which kind of protein secreted by Sertoli cells with different morphologies.
RESULTS AND CONCLUSION: There were 14 electrophoretic bands from the growth of cell adherence with flat Sertoli cells, and the molecular weight of protein distributed from 17×103 to 158×103. There were 10 electrophoretic bands from the cell growth in microcapsule with spherical Sertoli cells, and the molecular weight of protein distributed from 17×103 to 58×103. These findings suggest that there are differences between the quantity and the kind of protein which is secreted by Sertoli cells with different morphologies. There are obvious correlations between the morphologies of the cells cultured in vitro and their physiological function.

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