中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (33): 6107-6110.doi: 10.3969/j.issn.2095-4344.2012.33.006

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

成年与幼年猪半月板损伤愈合的体外实验

戴 祝1，杨乐忠2，陈志伟1，刘春磊1   

  1. 1南华大学附属第一医院骨科，湖南省衡阳市 421001；
    2永州市中心医院南院骨科，湖南省永州市 425006
  • 收稿日期:2011-12-09 修回日期:2012-01-09 出版日期:2012-08-12 发布日期:2013-07-15
  • 通讯作者: 杨乐忠，硕士，医师，永州市中心医院南院骨科，湖南省永州市 425006 toulangyingwang@163.com
  • 作者简介:戴祝★，男，1980年生，湖南省望城县人，汉族，2005年中南大学湘雅医学院毕业，硕士，主治医师，主要从事关节外科与运动医学研究。oliverdaizhu@sina.com.cn

In vitro experimental study on the healing of meniscus injury between mature and immature swines

Dai Zhu1, Yang Le-zhong2, Chen Zhi-wei1, Liu Chun-lei1   

  1. 1Department of Orthopedics, First Affiliated Hospital of the University of South China, Hengyang 421001, Hunan Province, China;
    2Department of Orthopedics, South Branch of Yongzhou Central Hospital, Yongzhou 425006, Hunan Province, China
  • Received:2011-12-09 Revised:2012-01-09 Online:2012-08-12 Published:2013-07-15
  • Contact: Yang Le-zhong, Master, Physician, Department of Orthopedics, South Branch of Yongzhou Central Hospital, Yongzhou 425006, Hunan Province, China toulangyingwang@163.com
  • About author:Dai Zhu★, Master, Attending physician, Department of Orthopedics, First Affiliated Hospital of the University of South China, Hengyang 421001, Hunan Province, China oliverdaizhu@sina.com.cn

PDF

520

被引次数

6

摘要：

背景：幼年半月板损伤后的愈合能力要优于成年半月板，以往认为是由于幼年半月板血运较成年半月板丰富。
目的：通过在体外器官培养模型中观察成年及幼年猪半月板损伤界面愈合的差异，探讨半月板损伤愈合的机制。
方法：6月龄成年猪、1月龄幼年猪处死后取双侧膝关节内、外侧半月板，每组32份标本，建立全层纵裂模型。标本行体外器官培养，培养0，2，4，6周后观察纵裂界面愈合情况并半定量评分。
结果与结论：成年组培养6周后，2份标本有少量修复组织产生。幼年组培养2周后，3份标本已有少量修复组织产生；4周后所有标本有修复反应，其中3份有桥接；6周后5份标本裂隙消失，其余3份有桥接。培养4，6周后，幼年组组织学半定量得分高于成年组(P < 0.05)；幼年组除0周与2周以外，其余各时间点比较差异均有显著性意义(P < 0.05)。说明体外器官培养模型排除了血运、滑膜、细胞因子等的影响，幼年半月板愈合优于成年半月板是由于其内在愈合能力强于成年半月板。

关键词: 半月板, 损伤, 器官培养模型, 愈合, 体外实验, 猪, 组织构建

Abstract:

BACKGROUND: The reason why the healing of immature meniscus injury is better than that of mature meniscus is the richer blood supply in immature meniscus than in mature meniscus in traditional opinion.
OBJECTIVE: To observe the differences in the interface healing of meniscus injury between mature and immature swine through in vitro organ culture models and to investigate the underlying mechanism of the healing of meniscus injury.
METHODS: The medial and lateral meniscuses were harvested from the posterior knees of local mature swine (6 months old) and local immature swine (1 month old) after death. The specimens were divided into mature group and immature group with 32 specimens in each group. A full-thickness tear model was established. The specimens were cultured in vitro for 0, 2, 4 and 6 weeks. Tear interface healing was investigated by using histology analysis and semi-quantitative scores were recorded.
RESULTS AND CONCLUSION: In the mature group, no reparative response was observed in all specimens except two specimens showed little reparative tissue at the interface at week 6 after culture. In the immature group: a few reparative tissues could be observed in three specimens at week 2 after culture; reparative response was observed in all specimens and bridge linking could be found in three specimens at week 4 after culture; the defects vanished in five specimens and bridge linking could be found in another three specimens at week 6 after culture. Semi-quantitative scores in the immature group were both higher than those in the mature group at weeks 4 and 6 after culture (P < 0.05). Significant differences could be found at all the time points except for 0 and 2 weeks (P < 0.05). These findings suggest that excluding the influence of vascular supply, synovium and cell factors in in vitro organ culture models, the reason why the healing potential in the immature meniscus is superior may contribute to its better meniscal healing than mature meniscus.

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