中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (19): 3496-3501.doi: 10.3969/j.issn.1673-8225.2012.19.016

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

自体脂肪间充质干细胞复合人脐带Wharton胶支架修复兔膝关节软骨缺损****★◇

韩  爽1，卢世璧2◇，刘  强1，张  莉2，黄靖香2，汪爱媛2，刘舒云2，赵  斌2，许文静2，郭全义2   

  1. 1山西医科大学第一临床医学院骨科，山西省太原市  030001；2解放军总医院骨科研究所，北京市100853
  • 收稿日期:2012-04-08 修回日期:2012-04-19 出版日期:2012-05-06 发布日期:2012-05-06
  • 通讯作者: 郭全义，博士，副主任医师，解放军总医院骨科研究所，北京市 100853 doctorguo@163.com
  • 作者简介:韩爽★，男，1985年生，陕西省西安市人，汉族，山西医科大学第一临床医学院骨科在读硕士，主要从事软骨组织工程研究。 luckydoghs@163.com
  • 基金资助:

    国家自然科学基金资助项目(81071458，81000810，30973047)，863计划项目(SS2012AA022106)。

Autologous adipose derived stem cells loaded onto human umbilical cord Wharton’s Jelly matrix-derived scaffold for repair of full-thickness articular cartilage defects of the knee in rabbits

Han Shuang1, Lu Shi-bi2◇, Liu Qiang1, Zhang Li2, Huang Jing-xiang2, Wang Ai-yuan2, Liu Shu-yun2, Zhao Bin2, Xu Wen-jing2, Guo Quan-yi2    

  1. 1Department of Orthopedics, First Clinical Medical College, Shanxi Medical University, Taiyuan  030001, Shanxi Province, China; 2Institute of Orthopedics, General Hospital of Chinese PLA, Beijing  100853, China
  • Received:2012-04-08 Revised:2012-04-19 Online:2012-05-06 Published:2012-05-06
  • Contact: Guo Quan-yi, M.D., Associate chief physician, Institute of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China
  • About author:Han Shuang★, Studying for master’s degree, Department of Orthopedics, First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China luckydoghs@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81071458*, 81000810*,  30973047*; National High-Tech Research and Development Program of China (863 Program), No. SS2012AA022106*

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摘要：

背景：脐带Wharton胶富含透明质酸，糖胺多糖及胶原等，成分与天然软骨细胞外基质类似，因此由人脐带提取的Wharton胶很可能是一种较为理想的软骨组织工程支架材料。
目的：评价自体脂肪间充质干细胞复合人脐带Wharton胶支架修复兔膝关节软骨缺损的效果。
方法：将终浓度为1010 L -1、成软骨方向诱导后的兔自体脂肪间充质干细胞与人脐带Wharton胶支架复合，继续培养1周构建组织工程软骨，对兔膝关节全层软骨缺损进行修复(实验组)，并与单纯支架修复的对照组及空白组进行比较。术后3个月对修复组织行大体观察、组织学检测、糖胺多糖、总胶原定量检测及生物力学测定。
结果与结论：实验组的缺损多为透明软骨修复，对照组以纤维组织修复为主，空白组无明显组织修复。提示脂肪间充质干细胞作为软骨组织工程种子细胞具有可行性；实验构建的组织工程软骨能有效的修复关节软骨缺损，人脐带Wharton胶可作为软骨组织工程良好的支架材料。

关键词: 人脐带Wharton胶支架, 脂肪间充质干细胞, 软骨缺损, 组织工程

Abstract:

BACKGROUND: Umbilical cord Wharton jelly matrix is rich in hyaluronic acid, glycosaminoglycan and collagen, with similar components to natural chondrocyte extracellular matrix. Therefore, human umbilical cord-extracted Wharton jelly matrix is likely to become an ideal tissue engineering scaffold material of cartilage tissue. 
OBJECTIVE: To evaluate the therapeutic effects of autologous adipose derived stem cells loaded onto human umbilical cord Wharton’s Jelly matrix-derived scaffold in repair of full-thickness articular cartilage defects of the knee in rabbits.
METHODS: After induced differentiation into chondrocytes, rabbit auatologous adipose derived stem cells at a final concentration of 1010/L were compounded with human umbilical cord Wharton’s jelly matrix-derived scaffold and further cultured for 1 week to construct tissue-engineered cartilage. The full-thickness articular cartilage defects of the knee in rabbits were repaired by the tissue-engineered cartilage (experimental group). The therapeutic effects were compared between experimental group, scaffold group and blank group. At 3 months after surgery, gross observation, histological examination, glycosaminoglycan (GAG) and total collagen quantification and biomechanical tests were applied to analyze the results.
RESULTS AND CONCLUSION: In the experimental group, most of defects were repaired by hyaline cartilage. In the scaffold group, most of defects were repaired by fibrous tissue. In the blank group, the defects were repaired with less fibrous tissue or not repaired. It is feasible to use adipose derived stem cells as the seed cells of cartilage tissue engineering. The constructed cartilage by tissue engineering can effectively repair knee articular cartilage defects and human umbilical cord Wharton’s jelly matrix-derived scaffold can be used as a good cartilage tissue engineering scaffold.

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