中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (16): 2885-2888.doi: 10.3969/j.issn.1673-8225.2011.16.009

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

不同植骨材料在腰椎融合过程中的应用及转化生长因子β的表达

王磊磊，金格勒，任龙龙，杨  毅   

  1. 新疆医科大学第一附属医院VIP外二科，新疆维吾尔自治区乌鲁木齐市  830000
  • 收稿日期:2010-10-18 修回日期:2011-03-02 出版日期:2011-04-16 发布日期:2013-11-11
  • 通讯作者: 金格勒，男，博士，教授、主任医师，新疆医科大学第一附属医院VIP外二科，新疆维吾尔自治区乌鲁木齐市 830000 jingl81@yahoo.com.cn
  • 作者简介:王磊磊★，男，1984年生，山东省青岛市人，汉族，新疆医科大学在读硕士，主要从事非细胞型组织工程研究。 313670898@qq.com
  • 基金资助:

    新疆维吾尔自治区自然科学基金项目(2010211a40)，项目名称：新型非细胞型组织工程植入体内血管化的实验研究；国家自然科学基金资助项目(30760257)，项目名称：外源性BMP-2在兔脊柱融合中的成骨机制研究。

Application of different bone graft materials in the process of lumbar fusion and transforming growth factor-beta expression

Wang Lei-lei, Jin Ge-le, Ren Long-long, Yang Yi   

  1. Second Department of VIP Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830000, Xinjiang Uygur Autonomous Region, China
  • Received:2010-10-18 Revised:2011-03-02 Online:2011-04-16 Published:2013-11-11
  • Contact: Jin Ge-le, Doctor, Professor, Chief physician, Second Department of VIP Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China jingl81@yahoo.com.cn
  • About author:Wang Lei-lei★, Studying for master’s degree, Second Department of VIP Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China 313670898@qq.com
  • Supported by:

    the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2010211a40*; the National Natural Science Foundation of China, No. 30760257*

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摘要：

背景：骨形态发生蛋白单独应用于骨移植效果不佳。而转化生长因子β能明显增强其在体内的诱导成骨作用。
目的：观察自体骨及重组人骨形态发生蛋白2复合骨用于兔腰椎融合过程中转化生长因子β的基因表达。
方法：将新西兰大白兔60只随机分为自体骨组、复合骨组和异体骨组，分别于双侧L5-6横突间植入自体骨、重组人骨形态发生蛋白2复合骨及异体骨。植入后7，14，21，28，35 d取3组节段骨痂，应用实时荧光定量RT-PCR检测转化生长因子β的基因表达水平。
结果与结论：植入后28 d，复合骨组转化生长因子β达峰值，随后有所下降，但均高于自体骨组和异体骨组(P < 0.05)。结果证实，重组人骨形态发生蛋白2复合骨缓释重组人骨形态发生蛋白2，有效地促进了转化生长因子β的表达，明显增强了体内的诱导成骨效应。

关键词: 人骨形态发生蛋白2, 转化生长因子&beta, 实时荧光定量逆转录聚合酶链反应, 腰椎, 生物材料

Abstract:

BACKGROUND: The effect of bone morphogenetic protein (BMP) used to bone transplantation is not ideal, while transforming growth factor-β (TGF-β) can significantly enhance osteoconductivity in vivo of BMP.
OBJECTIVE: To observe the TGF-β expression on autogenous bone and recombinant human bone morphogenetic protein-2 (rhBMP-2) used for the process of the lumbar fusion of rabbit.
METHODS: Sixty adult male New Zealand white rabbits were randomly divided into autograft group, composite group and allograft group. The rabbits were undergone L5-6 intertransverse process fusion respectively using autograft, rhBMP-2/composite bone and allograft. 3 segmental calluses were obtained at 7, 14, 21, 28, 35 days after implantation, the TGF-β expression was detected by real time fluorescent quantitation RT-PCR.
RESULTS AND CONCLUSION: At 28days after implantation, the TGF-β expression in composite group reached the peak, and then declined, but still had statistical difference compared to the other two groups (P < 0.05). RhBMP-2/composite allograft released rhBMP-2; meanwhile, the rhBMP-2 effectively improved the TGF-β expression and significantly increase osteoinductive effect in vivo.

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