中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (42): 6324-6329.doi: 10.3969/j.issn.2095-4344.2016.42.014

• 神经组织构建 nerve tissue construction • 上一篇    下一篇

神经细胞分泌因子促进骨骼肌细胞生长

蔡明轩1，王美辰1，徐治成1，颜  南2，孟欣雨1，王正东3   

  1. 沈阳医学院，1基础医学院，2康复教研室，3解剖学教研室，辽宁省沈阳市  110034
  • 收稿日期:2016-08-30 出版日期:2016-10-14 发布日期:2016-10-14
  • 通讯作者: 王正东，博士，沈阳医学院解剖学教研室，辽宁省沈阳市 110034
  • 作者简介:蔡明轩，女，1995年生，辽宁省人，汉族，主要从事周围神经损伤的治疗工作。
  • 基金资助:

    辽宁省大学生创新创业训练计划项目(20151161)；沈阳医学院大学生科研项目(20150801)

Neuron-secreted factors promote the growth of skeletal muscle cells

Cai Ming-xuan1, Wang Mei-chen1, Xu Zhi-cheng1, Yan Nan2, Meng Xin-yu1, Wang Zheng-dong3   

  1. 1Basic Medical School, 2Department of Rehabilitation, 3Department of Anatomy, Shenyang Medical University, Shenyang 110034, Liaoning Province, China
  • Received:2016-08-30 Online:2016-10-14 Published:2016-10-14
  • Contact: Wang Zheng-dong, M.D., Department of Anatomy, Shenyang Medical University, Shenyang 110034, Liaoning Province, China
  • About author:Cai Ming-xuan, Basic Medical School, Shenyang Medical University, Shenyang 110034, Liaoning Province, China
  • Supported by:

    the Innovative Training Program for College Students of Liaoning Province, No. 20151161; the College Students Scientific Research Project of Shenyang Medical University, No. 20150801

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342

被引次数

1

摘要：

文章快速阅读：

文题释义：
肌卫星细胞：骨骼肌中除骨骼肌纤维(肌细胞)外的一种扁平、有突起的细胞。附着于肌纤维(肌细胞)表面；当肌纤维(肌细胞)受损后，肌卫星细胞可增殖分化，参与肌纤维(肌细胞)的修复，因此具有干细胞性质。
骨骼肌细胞：肌细胞呈纤维状，不分支，有明显横纹，核很多，且都位于细胞膜下方。肌细胞内有许多沿细胞长轴平行排列的细丝状肌原纤维。每个肌原纤维都有相间排列的明带(Ⅰ带)及暗带(A带)。
摘要
背景：周围神经损伤可引起支配肌肉萎缩，目前尚无明确的方法治疗肌肉萎缩。肌细胞是实现肌肉收缩功能的结构单位，体外培养的骨骼肌细胞可用于失神经肌肉萎缩相关研究。
目的：观察神经元分泌因子对体外骨骼肌细胞的生长的促进作用。
方法：将体外原代培养的骨骼肌细胞分为2组，实验组加神经细胞分泌因子培养，对照组加普通培养液培养，用苏木精-伊红染色、免疫组织化学染色检测神经元分泌因子作用后骨骼肌细胞数目及α肌动蛋白表达的变化。
结果与结论：①骨骼肌细胞数量、骨骼肌细胞肌动蛋白的表达：实验组骨骼肌细胞数量、骨骼肌细胞α肌动蛋白的表达均多于对照组(P < 0.05)；②结果说明：神经元分泌因子有促进大鼠骨骼肌细胞生长的作用，可能具有治疗失神经肌肉萎缩的作用。
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID:0000-0002-5462-4452(王正东)

关键词: 组织构建, 组织工程, 骨骼肌细胞, 细胞培养, α肌动蛋白, 神经元, 促进, 分泌因子, 肌肉萎缩, 肌卫星细胞, 周围神经损伤

Abstract:

BACKGROUND: There is no effective treatment for muscle atrophy caused by peripheral nerve damage. Skeletal muscle cells, a structural unit of muscle contraction, can be used for studies on muscle atrophy when cultured in vitro.
OBJECTIVE: To investigate the promotion effect of neuron-secreted factors on the growth of skeletal muscle cells in vitro.
METHODS: Skeletal muscle cells primary cultured in vitro were divided into two groups: experimental group with neuron-secreted factors, and control group with common culture medium, respectively. Afterwards, the number of skeletal muscle cells and expression level of alpha actin were detected by hematoxylin-eosin staining and immunohistochemical staining, respectively.
RESULTS AND CONCLUSION: The number of skeletal muscle cells and expression level of alpha actin in the experimental group were significantly higher than those in the control group (P < 0.05). In conclusion, neuron-secreted factors have the ability of promoting the growth of skeletal muscle cells and may be helpful for denervated muscle atrophy.
Subject headings: Tissue Engineering; Satellite Cells, Skeletal Muscle; Actinin; Neurons; Cells, Cultured

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

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