中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (40): 7460-7464.doi: 10.3969/j.issn.2095-4344.2012.40.010

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

玻璃化冷冻保存组织工程肌腱植入大鼠体内的组织学变化

彭 蔷1，朱明华1，孙 涛1，曾 怡1，王 林1，刘程俊2，秦廷武2   

  1. 1四川省疾病预防控制中心，四川省成都市 610041
    2四川大学华西医院生物治疗国家重点实验室•干细胞与组织工程研究室，四川省成都市 610041
  • 收稿日期:2012-01-31 修回日期:2012-04-11 出版日期:2012-09-30 发布日期:2012-09-30
  • 通讯作者: 秦廷武，教授，四川大学华西医院生物治疗国家重点实验室?干细胞与组织工程研究室，四川省成都市 610041 tingwuqin@ hotmail.com
  • 作者简介:彭蔷，女，1964年生，四川省成都市人，汉族，1986年泸州医学院毕业，研究员，主要从事医疗器械的生物学评价及研究。 Pengq86@126. com

Histological changes in rats after implantation of vitreous-cryopreservation tissue engineered tendon

Peng Qiang1, Zhu Ming-hua1, Sun Tao1, Zeng Yi1, Wang Lin1, Liu Cheng-jun2, Qin Ting-wu2   

  1. 1Sichuan Center for Disease Control and Prevention, Chengdu 610041, Sichuan Province, China
    2Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2012-01-31 Revised:2012-04-11 Online:2012-09-30 Published:2012-09-30
  • Contact: Qin Ting-wu, Professor, Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China tingwuqin@hotmail. com
  • About author:Peng Qiang, Researcher, Sichuan Center for Disease Control and Prevention, Chengdu 610041, Sichuan Province, China Pengq86@126.Com

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摘要：

背景：应用玻璃化冷冻方法保存组织工程肌腱具有可行性和应用前景，有待进一步研究。
目的：探讨应用玻璃化冷冻保存组织工程肌腱体内植入对大鼠跟腱缺损修复的影响。
方法：选用成年SD大鼠64只，于大鼠跟腱中段制备5 mm肌腱缺损模型，随机摸球法均分为2组，分别植入玻璃化冷冻保存组织工程肌腱和新鲜非冷冻保存组织工程肌腱。植入后2，4，6，8周，观察植入肌腱材料及周围组织的大体形态和组织学变化。
结果与结论：两组肌腱材料体内植入后的大体形态和组织学反应无明显差异。植入后2，4周，植入的肌腱材料发生降解，材料中间及周围有大量炎性细胞浸润和纤维结缔组织增生。植入后6，8周，大量新生胶原纤维组织长入并替代降解的植入材料，形态和排列方式趋近于正常肌腱组织，大鼠跟腱缺损基本修复。结果表明玻璃化冷冻保存组织工程肌腱体内植入可修复大鼠跟腱缺损。

关键词: 组织工程, 肌腱, 玻璃化冷冻保存, 植入, 组织学

Abstract:

BACKGROUND: The technique of vitreous cryopreservation applied to preserve tissue engineered tendon has the practicability and application prospects, and is required for further research.
OBJECTIVE: To investigate the effects of in vivo implantation of vitreous-cryopreservation tissue engineered tendon on repairing the tendon defects of the rats.
METHODS: Sixty-four adult Sprague Dawley rats were randomly divided into two groups, vitreous-cryopreservation tissue engineered tendons and fresh tissue engineered tendons without cryopreservation were implanted respectively. The 5 mm tendon defects models were prepared in the middle part of Achilles tendons of the rats, and the tissue engineered tendons were implanted. At 2, 4, 6 and 8 weeks after surgery, the general morphology and histological changes of the implanted tendons and surrounding tissues were observed.
RESULTS AND CONCLUSION: No significant differences in general morphology and histological change of the implanted tendons and surrounding tissues were observed between two groups. At 2 and 4 weeks after surgery, the implanted tendon materials were degraded gradually, and were infiltrated and surrounded by a large number of inflammatory cells and proliferous fibrous connective tissues. At 6 and 8 weeks after surgery, the implanted tendons were degraded and replaced by a large number of regenerative collagenous fiber tissues; the morphology and orientation of the collagenous fiber tissues were similar to those of normal tendon tissues. The tendon defects of the rats were repaired basically. The results indicate the in vivo implantation of vitreous-cryopreservation tissue engineered tendons can repair the tendon defects of the rats.

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