中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7123-7126.doi: 10.3969/j.issn.1673-8225.2011.38.021

• 药物控释材料 drug delivery materials • 上一篇    下一篇

原位定型微囊化载体制剂在糖尿病大鼠皮肤溃疡中的作用

王晓梅1，王  府2，杨晓辉2，金成吉1，李  湘1   

  1. 1大连大学附属中山医院内分泌科，辽宁省大连市   116001
    2金乡县人民医院内分泌科，山东省济宁市  272200
  • 收稿日期:2011-03-07 修回日期:2011-04-18 出版日期:2011-09-17 发布日期:2011-09-17
  • 作者简介:王晓梅，女，1962年生，辽宁省大连市人，汉族，1984年大连医科大学毕业，主任医师，主要从事糖尿病慢性并发症的病理生理机制研究。 wangxiaomei1962@126.com

Therapeutic effect of microencapsulation carrier preparation for in situ stereotype on skin ulcers of diabetic rats

Wang Xiao-mei1, Wang Fu2, Yang Xiao-hui2, Jin Cheng-ji1, Li Xiang1   

  1. 1Department of Endocrinology and Metabolism, Zhongshan Hospital Affiliated to Dalian University, Dalian  116001, Liaoning Province, China
    2Department of Endocrinology, People’s Hospital of Jinxiang County, Jining  272200, Shandong Province, China
  • Received:2011-03-07 Revised:2011-04-18 Online:2011-09-17 Published:2011-09-17
  • About author:Wang Xiao-mei, Chief physician, Department of Endocrinology and Metabolism, Zhongshan Hospital Affiliated to Dalian University, Dalian 116001, Liaoning Province, China wangxiaomei1962@ 126.com

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277

被引次数

2

摘要：

背景：原位定型微囊化载体制剂成分之一胰岛素可以促进溃疡愈合。
目的：观察原位定型微囊化载体制剂在糖尿病大鼠皮肤溃疡中的疗效。
方法：腹腔内注射链脲佐菌素建立糖尿病大鼠模型，应用外科方法建立全层皮肤缺损模型。根据皮肤溃疡处干预方式将实验动物分为4组。①空白对照组用生理盐水处理创面。②一般制剂组应用甲硝唑+山莨菪硷1+普通短效胰岛素处理创面。③单纯微囊组创面外敷不含有效药物成分的微囊化载体膜。④微囊化有效制剂组溃疡处外涂微囊化载体制剂膜，内含药物成分与一般制剂组相同。定时测量溃疡面积，记录溃疡愈合时间，取创面全层组织进行组织学观察，测定表皮生长因子受体、纤维连接蛋白阳性细胞数量。
结果与结论：微囊化有效制剂组大鼠溃疡愈合时间短于其他3组(P < 0.05或P < 0.01)，微囊化有效制剂组表皮生长因子受体和纤维连接蛋白阳性细胞数目高于其他各组(P < 0.05或P < 0.01)。结果表明，原位定型微囊化载体制剂能够缩短愈合时间和促进糖尿病大鼠皮肤溃疡愈合。

关键词: 微囊化载体制剂, 糖尿病大鼠, 皮肤溃疡, 表皮生长因子受体, 连接蛋白

Abstract:

BACKGROUND: Insulin as one of the components of microencapsulation carrier preparation for in situ stereotype can promote the ulcer healing.
OBJECTIVE: To evaluate the therapeutic effect of microencapsulation carrier preparation for in situ stereotype on skin ulcers of diabetic rats.
METHODS: Sprague-Dawley (SD) rats were used to establish diabetic rat models by intraperitoneal injection of streptozotocin (STZ) and to set up skin ulcer models by surgical methods. All rats were randomly divided into 4 groups by different interventions:  microencapsulation preparation with effective component group (MPE), microencasultion preparation without effective component group (MPNE), general preparation with effective component group (GE), blank control group (BC). Ulcer area was measured at regular time intervals. In the first 14 days, full-thickness skin wounds were brought to make histological observation and determine the positive cell numbers expressing epidermal growth factor receptor (EGFR) and fibronectin (FN) .
RESULTS AND CONCLUSION: The skin ulcer healing time of MPE group was the shortest compared with the other three groups (P < 0.05 or P < 0.01). The number of EGFR and FN positive cells in MPE group was more than that of any other three groups (P < 0.05 or P < 0.01). Microencapsulation carrier preparation for in situ stereotype can promote the healing course of skin ulcer in diabetic rats.

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