中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (34): 6339-6341.doi: 10.3969/j.issn.1673-8225.2011.34.017

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

纯镁及两种不同涂层纯镁的溶血现象

赵  刚1，吴艳娟1，胡  津2，刘广义2，李德超1   

  1. 1佳木斯大学口腔医学院，黑龙江省佳木斯市 154007
    2哈尔滨工业大学材料科学与工程学院，黑龙江省哈尔滨市 150001
  • 收稿日期:2011-02-10 修回日期:2011-03-10 出版日期:2011-08-20 发布日期:2011-08-20
  • 通讯作者: 李德超，博士，教授，佳木斯大学口腔医学院种植科，黑龙江省佳木斯市 154007 dechaoli2004@yahoo.com.cn
  • 作者简介:赵刚★，男，1975年生，黑龙江省依兰县人，硕士，副教授，主要从事牙缺损、缺失及牙颌畸形的修复与矫治研究。 zhaogangkq@126.com
  • 基金资助:

    黑龙江省卫生厅科技项目(2009-36)；佳木斯大学科学技术研究项目(S22009-001)。

Hemolysis phenomenon of pure magnesium and two different coating pure magnesium

Zhao Gang1, Wu Yan-juan1, Hu Jin2, Liu Guang-yi2, Li De-chao1   

  1. 1School of Stomatology, Jiamusi University, Jiamusi  154007, Heilongjiang Province, China
    2School of Materials Science and Engineering, Harbin Institute of Technology, Harbin  150001, Heilongjiang Province, China
  • Received:2011-02-10 Revised:2011-03-10 Online:2011-08-20 Published:2011-08-20
  • Contact: Li De-chao, Doctor, Professor, School of Stomatology, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China dechaoli2004@yahoo.com.cn
  • About author:Zhao Gang★, Master, Associate professor, School of Stomatology, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China zhaogangkq@126.com
  • Supported by:

    Science and Technology Project of Health Department of Heilongjiang Province, No. 2009-36*; Science and Technology Research Project of Jiamusi University, No. S22009-001*

PDF

388

被引次数

19

摘要：

背景：尽管作为植入材料镁具有良好的生物相容性及可降解吸收等优点，但镁腐蚀过快，它的快速腐蚀将引起其机械性能在骨组织康复之前就显著下降。
目的：检测纯镁及微弧氧化、二水磷酸氢钙涂层后的纯镁的血液相容性。
方法：将纯镁及微弧氧化、二水磷酸氢钙两种涂层后的纯镁浸提液与抗凝稀释兔血接触，阴性对照组用为生理盐水，阳性对照为蒸馏水。混匀后离心，用分光光度计测定其上清液的A值，计算溶血率。
结果与结论：未经处理的纯镁浸提液组溶血率为52.34%，微弧氧化涂层后的纯镁浸提液组溶血率为0.32%，二水磷酸氢钙涂层后的纯镁浸提液组溶血率为0.14%，阴性对照组溶血率为0，阳性对照组溶血率为100%。提示未经处理的纯镁出现了较严重的溶血现象，涂层后的纯镁血液相容性良好，溶血率小于5%，符合国家标准。

关键词: 纯镁, 微弧氧化, 二水磷酸氢钙, 溶血率, 口腔生物材料

Abstract:

BACKGROUND: Although magnesium as implant material which has good biocompatibility and biodegradable absorption. However, excessive corrosion of magnesium will cause rapid decrease of the mechanical properties before rehabilitation of bone tissue.
OBJECTIVE: To detect the blood-compatibility of pure magnesium (MG), micro-arc oxidized magnesium (MAO) and dicalcium phosphate dihydrate coating formed on micro-arc oxidized magnesium (DCPD).
METHODS: MG, MAO and DCPD extracts were contacted with anticoagulation dilution rabbit blood. Sodium Chloride was used in negative control group; distilled water was used in positive control group. Centrifuged after mixing, the optical density value of supernatant was determined by spectrophotometer, and hemolytic rate was counted.
RESULTS AND CONCLUSION: Hemolytic rate of untreated pure magnesium extract group was 52.34%; the MAO and DCPDC extract group were 0.32% and 0.14%. Hemolytic rate of negative and positive group were 0% and 100%. The untreated pure magnesium showed a serious hemolysis. However, MAO and DCPD have excellent blood-compatibility, hemolytic rate is less than 5%, and they conform to the national standard requirement.

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