中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5145-5148.doi: 10.3969/j.issn.1673-8225.2011.28.004

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

关节软骨及骨破坏模型大鼠经盐酸氨基葡萄糖干预的最佳剂量

王大利1，耿成燕2，姜丽平2，宫德正2，仲来福2   

  1. 1大连机车医院检验科，辽宁省大连市  116021
    2大连医科大学中日合作医药科学研究所，辽宁省大连市  116044
  • 收稿日期:2011-02-09 修回日期:2011-05-19 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 仲来福，教授，博士生导师，大连医科大学中日合作医药科学研究所，辽宁省大连市116044 zhong@dlmedu.edu.cn
  • 作者简介:王大利★，男，1969年生，辽宁省大连市人，汉族，1994年大连医科大学大学毕业，硕士，副主任技师，主要从事医学检验工作。 并列第一作者：耿成燕，博士，高级实验师。
  • 基金资助:

    课题受日本国日中替代医疗食品研究会项目(20050401)资助。

Minimal effective dose of glucosamine hydrochloride on experimental arthritis in rats

Wang Da-li1, Geng Cheng-yan2, Jiang Li-ping2, Gong De-zheng2, Zhong Lai-fu2   

  1. 1Department of Laboratory, Dalian Locomotive Hospital, Dalian  116021, Liaoning Province, China
    2China-Japanese Joint Institute for Medical and Pharmaceutical Science, Dalian Medical University, Dalian  116044, Liaoning Province, China
  • Received:2011-02-09 Revised:2011-05-19 Online:2011-07-09 Published:2011-07-09
  • Contact: Zhong Lai-fu, Professor, Doctoral supervisor, China-Japanese Joint Institute for Medical and Pharmaceutical Science, Dalian Medical University, Dalian 116044, Liaoning Province, China
  • About author:Wang Da-li★, Master, Associate chief technician, Department of Laboratory, Dalian Locomotive Hospital, Dalian 116021, Liaoning Province, China Geng Cheng-yan, Doctor, China-Japanese Joint Institute for Medical and Pharmaceutical Science, Dalian Medical University, Dalian 116044, Liaoning Province, China zhong@dlmedu.edu.cn Wang Da-li and Geng Cheng-yan contributed equally to this work
  • Supported by:

    a grant by Japan- China Substitute Medical Food Association, No. 20050401*

PDF

400

被引次数

12

摘要：

背景：研究表明盐酸氨基葡萄糖可减轻骨关节炎的症状并能保护关节软骨，但该药的最佳剂量至今尚无共识。
目的：观察盐酸氨基葡萄糖对由白陶土与鹿角菜胶诱发大鼠关节炎的治疗最低有效剂量。
方法：将SD大鼠分别用蒸馏水，盐酸氨基葡萄糖溶液0.4，0.8及1.6 g/kg灌胃，采用白陶土与鹿角菜胶诱发大鼠单发关节炎模型。建模后1，3，5 d，用容积测量法测定各组大鼠的左右后肢足跖体积，计算足跖肿胀度。用游标卡尺测定其胫跗骨关节最大径；测定足跖伊文思蓝含量，右足跖制备组织切片，染色后镜下观察其病变程度并进行评分。
结果与结论：给予大鼠盐酸氨基葡萄糖1，3和5 d后，盐酸氨基葡萄糖0.8及1.6 g/kg组大鼠右后足跖肿胀度及胫跗骨关节径明显小于模型组(P < 0.05或P < 0.01)，足跖肿胀度及胫跗骨关节径均有随给药剂量增大而减小。给药5 d后，盐酸氨基葡萄糖0.8及1.6 g/kg组大鼠右后足跖伊文思蓝含量及组织病理学评分明显小于模型组(P < 0.05或P < 0.01)。结果提示，盐酸氨基葡萄糖能够有效地预防骨关节炎关节软骨和骨的破坏，并可预防关节周围组织炎症，其最低有效剂量为0.8 g/kg。

关键词: 盐酸氨基葡萄糖, 白陶土, 鹿角菜胶, 骨关节炎, 大鼠, 骨组织工程

Abstract:

BACKGROUND: Studies have shown that glucosamine hydrochloride (GAH) may lessen the symptoms of osteoarthritis and protect articular cartilage, however, its optimal dose remains unclear.
OBJECTIVE: To observe the minimal effective dose of GAH on the kaolin and carrageenan-induced arthritis in rat models.
METHODS: Rats in experimental groups received distilled water (model control) or GAH at 0.4, 0.8 and 1.6 g/kg for  1 hour, respectively, twice daily for 5 consecutive days. The arthritis was induced by kaolin and carrageenan 1 hour after administration of GAH. The volume (mL) of hind paws for rats in each group was estimated 1, 3, 5 days after induction of arthritis. The degree of right hind paw swelling was calculated. The maximal diameter of tibio-tarsal articulation was measured with a vernier caliper. The concentration of Evans blue was also determined. The histopathlolgical severity of right hind paw in arthritis modes was evaluated and scored.
RESULTS AND CONCLUSION: The degree of hind paw swelling of right hind paw and diameter of tibio-tarsal articulation showed a statistically significant reduction in rats given GAH 0.8 and 1.6 g/kg compared with rats in model control at 1, 3, 5 days (P < 0.05 or P < 0.01), exerting a dose-dependent manner. The content of Evans blue and histopathological scores significantly decreased in rats given GAH 0.8 and 1.6 g/kg for 5 days compared with model control group (P < 0.05 or P < 0.01). GAH is effective in preventing destruction of cartilage bone and inflammation of soft tissue around articulation in the rat model of kaolin and carrageenan-induced arthritis, and the minimal effective dose is 0.8 g/kg.

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