中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (19): 3531-3533.doi: 10.3969/j.issn.1673-8225.2011.19.026

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

旋转式三维细胞培养装置的研制

赵灵犀1，丁  皓1，2，徐  萌1，尚  昆2，沈力行1，2   

  1. 1上海理工大学医疗器械与食品学院，上海市200093
    2上海医疗器械高等专科学校，上海市200093
  • 收稿日期:2010-11-28 修回日期:2011-03-16 出版日期:2011-05-07 发布日期:2011-05-07
  • 通讯作者: 丁皓，博士，副教授，硕士生导师。上海医疗器械高等专科学校，上海市200093 dinghaoy@21cn.com
  • 作者简介:赵灵犀★，女，1986年生，重庆市人，汉族，上海理工大学在读硕士，主要从事生物力学研究。 lingxi_zhao@126.com
  • 基金资助:

    上海理工大学医疗器械与食品学院微创励志创新基金，课题名称：三维内皮细胞培养装置研制与实验。

The development of device for rotary three-dimensional cells culture 

Zhao Ling-xi1, Ding Hao1,2, Xu Meng1, Shang Kun2, Shen Li-xing1,2   

  1. 1Institute of Biomechanics and Rehabilitative Engineering, University of Shanghai for Science and Technology, Shanghai  200093, China
    2Shanghai Medical Instrumentation College, Shanghai 200093, China
  • Received:2010-11-28 Revised:2011-03-16 Online:2011-05-07 Published:2011-05-07
  • Contact: Ding Hao, Doctor, Associate professor, Master’s supervisor, Institute of Biomechanics and Rehabilitative Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; Shanghai Medical Instrumentation College, Shanghai 200093, China dinghaoy@21cn.com
  • About author:Zhao Ling-xi★, Studying for master’s degree, Institute of Biomechanics and Rehabilitative Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China lingxi_zhao@126.com
  • Supported by:

    microinvasive Inspira tional Innovation Fund, Institute of Biomechanics and Rehabilitative Engineering, University of Shanghai for Science and Technology*

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摘要：

背景：内皮细胞体外培养形成的单层内皮细胞能否抵抗血流的冲击，如何实现细胞均匀分布生长是实验中的常见问题。
目的：针对背景问题，研制一种细胞培养装置，可提供细胞生长的三维环境，实现细胞贴硅胶弹性腔管壁生长，改善细胞的分布不均。
方法：从控制单元、机械单元和细胞培养单元介绍了该装置的结构与功能，并利用该装置进行一例脐静脉内皮细胞培养实验。
结果与结论：旋转式三维细胞培养装置为细胞代谢提供了一定条件，该装置培养的细胞能贴壁均匀，生长良好。

关键词: 旋转培养, 内皮细胞, 细胞培养, 贴壁生长, 控制单元, 机械单元

Abstract:

BACKGROUND: It is widely used that vitro culturing endothelial cells to study atherosclerosis mechanism, and the key issues are if the monolayer endothelial cells could resist blood attack and how to achieve cells growth well-distributed.
The development of this device is aim to solve these problems.
OBJECTIVE: To develop a device that could supply a three-dimensional condition for cells to realize growth stick to elastic silicon chamber in order to improve growth uneven.
METHODS: It Weintroduced structure and functions of the device from controlled unit, mechanical unit and cells culture unit, and then used it to carry out an umbilical vein endothelial cells culture experiment.
RESULTS AND CONCLUSION: Rotary three-dimensional cells culture device could provide condition for cells metabolism and growth sticking to elastic silicon chamber well-distributed.

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