中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (19): 3438-3442.doi: 10.3969/j.issn.1673-8225.2011.19.004

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

新生鼠缺氧缺血性脑病时骨髓基质干细胞的生长特性

张  谊1，姚  笠2   

  1. 1首都医科大学附属北京同仁医院儿科，北京市   100730
    2哈尔滨医科大学附属第二医学院儿科，黑龙江省哈尔滨市   150086
  • 收稿日期:2010-11-20 修回日期:2010-12-29 出版日期:2011-05-07 发布日期:2011-05-07
  • 作者简介:张谊★，女，1976年生，北京市人，汉族，2004年哈尔滨医科大学毕业，硕士，主治医师，主要从事新生儿、儿童血液肿瘤研究。 Yichang-1@163.com

Growth characteristics of bone marrow stromal stem cells in neonatal rats with hypoxic-ischemic encephalopathy

Zhang Yi1, Yao Li2   

  1. 1Department of Pediatrics, Beijing Tongren Hospital, Capital Medical University, Beijing  100730, China
    2Department of Pediatrics, Second Affiliated Hospital of Harbin Medical University, Harbin  150086, Heilongjiang Province, China
  • Received:2010-11-20 Revised:2010-12-29 Online:2011-05-07 Published:2011-05-07
  • About author:Zhang Yi★, Master, Attending physician, Department of Pediatrics, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

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摘要：

背景：骨髓基质干细胞用于缺氧缺血性脑病治疗，其生长特性是否稳定尚需观察。
目的：探讨急性脑缺氧缺血新生大鼠骨髓基质干细胞移植后的生长状态及理化性质的改变。
方法：结扎新生7 d Wistar大鼠双侧颈总动脉制成缺血模型，恢复2 h后，吸入体积分数8%O2、92%N2的混合气体制成缺氧缺血性脑病模型。分为轻度(缺氧1 h)、中度(缺氧1.5 h)、重度(缺氧2.5 h)缺氧缺血性脑病组及假手术组(仅结扎左侧颈总动脉)。观察各组骨髓基质干细胞的生长状态。
结果与结论：假手术组及轻度缺氧缺血性脑病组骨髓基质干细胞在提取培养3 h即可贴壁生长，而中、重度组骨髓基质干细胞贴壁较晚且生长状态较差，假手术组、轻、中度组细胞形态可逐渐恢复。假手术组及轻度组细胞凋亡率基本相同，中、重度组细胞凋亡率明显增高。轻度及中度组培养上清液乳酸脱氢酶有不同程度的增高。提示轻、中度缺氧缺血对骨髓基质干细胞的影响是可逆的，骨髓基质干细胞生长旺盛，对于损伤恢复功能较强。

关键词: 缺氧缺血性脑病, 骨髓基质干细胞, 大鼠, 乳酸脱氢酶, 细胞凋亡

Abstract:

BACKGROUND: The growth features of bone marrow stromal stem cells (BMSCs) for hypoxia ischemic encephalopathy is unclear.
OBJECTIVE: To observe growth characteristics and physical and chemical characters of BMSCs in treatment of acute anoxic-ischemia newborn rats.
METHODS: Seven-day-old rats were selected to ligate the bilateral carotid arteries to prepare ischemic models. After 2 hours, the neonatal rats deeply suffered from hypoxia (8% O2 and 92% N2) for 1-2.5 hours. The rats were divided into mild group (ischemia for 1 hour), moderate group (ischemia for 1.5 hours), severe group (ischemia for 2.5 hours), anoxia-ischemia group and sham operation group (only ligation of the left carotid artery). The growth condition of BMSCs was observed.
RESULTS: After 3-hour culture, BMSCs grew adherently in the sham operation and anoxia-ischemia groups, superior to those in the moderate and severe groups. The morphology of BMSCs recovered in the sham operation, mild and moderate groups. The apoptotic rates were similar in the sham operation and mild groups, and obviously increased in the moderate and severe groups. The activity of supernatant lactate dehydrogenase was increased in the mild and severe groups to different extent. The findings demonstrate that the effect of mild and moderate anoxia-ischemia on BMSCs is reversible, and BMSCs grow vigorously and benefit to injury repair.

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