中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (18): 3341-3346.doi: 10.3969/j.issn.1673-8225.2011.18.027

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

30%小体积肝移植大鼠模型的手术技巧及改良

李  江1，刘  静2，侯  宇3，李  立2   

  1. 1昆明医学院第一附属医院肝胆外科，云南省昆明市   650032
    2昆明医学院附属甘美医院肝胆一科，云南省昆明市   650011
    3昆明医学院第三附属医院放疗科，云南省昆明市   650118
  • 收稿日期:2010-11-25 修回日期:2011-01-10 出版日期:2011-04-30 发布日期:2011-04-30
  • 通讯作者: 李立，博士，教授，主任医师，博士生导师，昆明医学院附属甘美医院肝胆一科，云南省昆明市 650011 llkmchina@yahoo.com.cn
  • 作者简介:李江☆，男，1975年生，云南省昆明市人，汉族，昆明医学院在读博士，主治医师，主要从事肝胆外科疾病及器官移植研究。 lijiangchina@ yahoo.com.cn
  • 基金资助:

    云南省科技厅重大项目(2007CA007)“多器官联合移植重大关键技术的研究及应用示范”。

Surgical technique improvement for liver transplantation in rats using 30% small-for-size grafts

Li Jiang1, Liu Jing2, Hou Yu3, Li Li2   

  1. 1Department of Hepatobiliary Surgery, First Affiliated Hospital of Kunming Medical University, Kunming  650032 , Yunnan Province, China
    2First Department of Hepatobiliary Surgery, Ganmeit Affiliated Hospital of Kunming Medical University, Kunming  650011, Yunnan Province, China
    3Department of Radiation Oncology, Third Affiliated Hospital of Kunming Medical University, Kunming  650118, Yunnan Province, China
  • Received:2010-11-25 Revised:2011-01-10 Online:2011-04-30 Published:2011-04-30
  • Contact: Li Li, Professor, Doctoral supervisor, First Department of Hepatobiliary Surgery, Ganmeit Affiliated Hospital of Kunming Medical University, Kunming 650011, Yunnan Province, China llkmchina@ yahoo.com.cn
  • About author:Li Jiang☆, Studying for doctorate, Department of Hepatobiliary Surgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032 , Yunnan Province, China lijiangchina@ yahoo. com.cn
  • Supported by:

     the Major Program of Yuman Science and Technology Comittee Province, No.2007CA007*

PDF

363

被引次数

3

摘要：

背景：活体肝移植显著缓解了供肝短缺矛盾，如何使供者献出的肝量最小，同时受者得到最大的临床受益成为了目前的研究热点。
目的：探索一种简便、稳定的小体积肝移植大鼠模型的建立方法。
方法：以Kamada“二袖套法”非动脉化原位肝移植SD大鼠模型为基础并参考国内外文献，制作30%小体积肝移植模型。实验分为Ⅰ组为体内肝叶减除；Ⅱ组为体外肝叶减除组；Ⅲ组为改良后小体积肝移植模型。主要观察供体手术各阶段时间；移植后并发症和生存情况。
结果与结论：改良后模型较改良前的供肝获取时间和肝叶减除时间均显著缩短(P< 0.05)。Ⅱ组的供肝冷缺血时间显著长于Ⅰ组和Ⅲ组(P < 0.05)。改良后的小体积肝移植模型手术成功率较改良前显著提高(P< 0.05)，改良后7及14 d存活率均高于改良前模型(P < 0.05)。改良模型较改良前生存时间延长了9 d。改良模型的手术总并发症例次较改良前组显著较少(P < 0.01)。提示采用此改良方法能建立稳定的30%小体积肝移植大鼠模型，而且在操作简便、减少并发症、提高手术成功率和术后存活率等方面有显著优势。

关键词: 小体积, 肝移植, 动物模型, 大鼠, 成功率, 并发症

Abstract:

BACKGROUND: The techniques of partial liver transplantation, using a living donor graft, expand the supply of organs and partially overcome the grafts shortage. But these benefits are limited in adult recipients when the volume of the grafts is small. To establish a model of small-for-size liver transplantation, using a simple and effective way, is the basis for the study mentioned above.
OBJECTIVE: To explore a simple and effective way of establishing a 30% small-for-size liver transplantation in rats.
METHODS: 280 Spraque-Dawley rats were selected as the donors and recipients to establish 30% small-for-size orthotropic live transplantation using two-cuff techniques. Animals were divided into two groups depending on the techniques modified or not. Sixty pairs of rats were divided into before modification group, using the way according to reference 10 to 15 and the median lobe of the liver as graft. These 60 pairs of rats were divided into two groups again depending on hepatectomy in vivo or in vitro. Group Ⅰ, performed hepatectomy in vivo before liver irrigation; groupⅡ, performed hepatectomy in vitro after liver irrigation. Eighty pairs of rats transplanted using the way of improvement by us were divided into modification group (group Ⅲ), in which hepatectomy was performed in suit after liver irrigation, the median and right lobes of liver were used as graft, body weights of donor were 100-120 g less than those of recipients, two-cuff technique and bile duct stent techniques were improved. Time of operation, survival and technical complications were compared among these groups.
RESULTS AND CONCLUSION: The time of hepatectomy and graft harvest were significantly shorter in group Ⅲ than those in group Ⅱ andⅠ(P < 0.05). The cold ischemia time in group Ⅱ was significantly longer than those in groupⅠand Ⅲ(P < 0.05). The incidence of bleeding, bile leakage IVC stricture, graft less perfusion and gas embolism were significantly less in group Ⅲ than those before modification. The rats in group Ⅲ had a higher transplanting successful rate, more 7-d and 14-d survivors post operation and longer median survival time (14 d) than those before modification (P < 0.05). The way of modification by us was a more effective and simple for establishing a 30% small-for-size liver transplantation in rats with higher transplanting successful rate and survival rate but fewer complications after operation.

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