中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (16): 2911-2914.doi: 10.3969/j.issn.1673-8225.2011.16.014

• 药物控释材料 drug delivery materials • 上一篇    下一篇

海藻糖保护同种带瓣大动脉的最适浓度

程晨晨，常  青，高洪波，徐  平   

  1. 青岛大学医学院附属医院心血管外科，山东省青岛市  266003
  • 收稿日期:2010-12-01 修回日期:2011-02-20 出版日期:2011-04-16 发布日期:2013-11-11
  • 通讯作者: 常青，博士，副主任医师，硕士生导师，青岛大学医学院附属医院心血管外科，山东省青岛市 266003 changqing20671@163.com
  • 作者简介:程晨晨★，男，1983年生，山东省滕州市人，汉族，青岛大学医学院在读硕士，主要从事冠心病﹑瓣膜病方面的研究。 xingchen-102@163.com
  • 基金资助:

    青岛市科技发展计划(06-2-2-6-nsh-1)，课题名称：海藻糖对同种带瓣大动脉组织的保护作用。

Optimal concentration of trehalose for protection of aortic valve homograft

Cheng Chen-chen, Chang Qing, Gao Hong-bo, Xu Ping   

  1. Department of Cardiovascular Surgery, the Affiliated Hospital of Qingdao University Medical College, Qingdao  266003, Shandong Province, China
  • Received:2010-12-01 Revised:2011-02-20 Online:2011-04-16 Published:2013-11-11
  • Contact: Chang Qing, Doctor, Associate chief physician, Master’s supervisor, Department of Cardiovascular Surgery, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China changqing20671@163.com
  • About author:Cheng Chen-chen★, Studying for master’s degree, Department of Cardiovascular Surgery, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China xingchen-102@163.com
  • Supported by:

    Technology and Development Planning of Qingdao, No, 06-2-2-6-nsh-1*

PDF

336

被引次数

3

摘要：

背景：目前液氮低温保护组织和器官技术已经非常成熟，但其保护剂一直以来争论不一。
目的：观察不同冷冻保护剂对液氮深低温冻存同种带瓣大动脉组织细胞凋亡和代谢的影响，寻求最佳的冷冻保护剂及冷冻保护剂的最适浓度。
方法：取新西兰大白兔带瓣主动脉、肺动脉标本，将其在液氮中冻存12，15，18个月，冻存液分别使用0.1 mol/L二甲基亚砜，0.1 mol/L海藻糖，0.1 mol/L海藻糖+0.1 mol/L二甲基亚砜，0.2 mol/L海藻糖+0.1 mol/L二甲基亚砜，0.3 mol/L海藻      糖+0.1 mol/L二甲基亚砜。标本复温后，免疫组织化学方法检测标本的细胞凋亡情况，葡萄糖消耗量测定法检测组织细胞的代谢水平。
结果与结论：免疫组织化学和葡萄糖消耗量测定结果均显示经0.1 mol/L海藻糖+0.1 mol/L二甲基亚砜，0.2 mol/L海藻糖+  0.1 mol/L二甲基亚砜冻存液处理的标本冻存效果最好，其次是经0.3 mol/L海藻糖+0.1 mol/L二甲基亚砜处理和经0.1 mol/L海藻糖单独处理的标本，单独使用0.1 mol/L二甲基亚砜处理的标本冻存效果最差。说明海藻糖对液氮深低温保存同种带瓣大动脉活性有很好的保护作用，单独使用海藻糖优于单独使用二甲基亚砜，联合应用海藻糖和二甲基亚砜效果更好，且联合应用的海藻糖最佳浓度范围是0.10~0.20 mol/L。

关键词: 海藻糖, 超低温保存, 同种瓣膜, 大动脉, 液氮, 冷冻保护剂

Abstract:

BACKGROUND: It was very mature that protecting organs and tissues in liquid nitrogen at present, but the debates were different protective agents of that.
OBJECTIVE: To observe the effect of apoptosis and metabolization of trehalose as cryoprotectant for preserving aortic valve homograft in liquid nitrogen on their tissues and cells, and to seek the best protective agents and their optimal concentrations.  
METHODS: The aortas and pulmonary arteries obtained from New Zealand white rabbits and frozen in liquid nitrogen to 12, 15, 18 months, 0.1 mol/L dimethyl sulfoxide (DMSO), 0.1 mol/L trehalose, 0.1mol/L trehalose +0.1 mol/L DMSO, 0.2 mol/L trehalose+0.1 mol/L DMSO, 0.3 mol/L trehalose+ 0.1 mol/L DMSO was used in liquid nitrogen, respectively. After specimens rewarming, the apoptosis of the specimens was detected by immunohistochemistry method, and the metabolism of tissue cells was detected by glucose consumption.
RESULTS AND CONCLUSION: Immunohistochemistry and glucose consumption determination results showed that the freezing effects of 0.1mol/L trehalose + 0.1 mol/L DMSO and 0.2 mol/L trehalose+0.1 mol/L DMSO were the best. 0.3 mol/L trehalose+  0.1 mol/L DMSO, which was worse than 0.1 mol/L trehalose + 0.1 mol/L DMSO and 0.2 mol/L trehalose+0.1 mol/L DMSO, ranked above 0.1 mol/L trehalose. The worst was 0.1 mol/L DMSO. The trehalose as the cryoprotectant for preserving aortic valve homograft in liquid nitrogen had a good protective effect. The separate use of trehalose was better than the separate use of DMSO. The joint use of trehalose and DMSO was better than the separate use of either one. The best concentrations of trehalose of the joint use were between 0.1 mol/L and 0.2 mol/L.

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