中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (21): 3975-3978.doi: 10.3969/j.issn.1673-8225.2011.21.042

• 药物控释材料 drug delivery materials • 上一篇    下一篇

海藻糖对低温保存胸骨中bcl-2和bax基因表达的影响

江玉华1，徐林浩2，罗宜人2，姚如勇3，王勇杰2   

  1. 1青岛大学医学院，山东省青岛市  266021；青岛大学医学院附属医院，2胸外科，3中心实验室，山东省青岛市  266003
  • 收稿日期:2010-11-12 修回日期:2010-12-12 出版日期:2011-05-21 发布日期:2011-05-21
  • 通讯作者: 徐林浩，副主任医师，青岛大学医学院附属医院胸外科，山东省青岛市 266003
  • 作者简介:江玉华★，男，1983年生，山东省淄博市人，汉族，青岛大学医学院在读硕士，主要从事胸部疾病的研究。
  • 基金资助:

    青岛市科技局课题资助项目(06-2-2-6-nsh-2)。

Effects of trehalose on bcl-2 and bax mRNA expression in the cryopreserved sternum

Jiang Yu-hua1, Xu Lin-hao2, Luo Yi-ren2, Yao Ru-yong3, Wang Yong-jie2   

  1. 1Qingdao University Medical College, Qingdao  266021, Shandong Province, China; 2Thoracic Surgery, 3Central Laboratory, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China
  • Received:2010-11-12 Revised:2010-12-12 Online:2011-05-21 Published:2011-05-21
  • Contact: Xu Lin-hao, Associate chief physician, Thoracic Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China jiangyuhua1983@163.com
  • About author:Jiang Yu-hua★, Studying for master’s degree, Qingdao University Medical College, Qingdao 266021, Shandong Province, China jiangyuhua123.-@163.com
  • Supported by:

    a grant from Science and Technology Bureau of Qingdao City, No. 06-2-2-6-nsh-2*

PDF

322

被引次数

12

摘要：

背景: 研究已经证实海藻糖对低温保存中的胸骨具有保护作用，但作用途径尚不清楚。
目的：观察海藻糖对低温保存胸骨bcl-2和bax基因表达的影响。
方法：新鲜配置4组保存液：低钾右旋糖酐组、低钾右旋糖酐+二甲基亚砜组、低钾右旋糖酐＋海藻糖组、低钾右旋糖酐＋二甲基亚砜组＋海藻糖组。切取SD大鼠胸骨后立即分别放入含上述4 种溶液的冻存管中低温保存。抽取新鲜大鼠胸骨组织和低温保存120 d的4组标本，采用RT-PCR方法检测不同保存液保存的胸骨及新鲜胸骨bcl-2和bax基因mRNA表达量。
结果与结论：低钾右旋糖酐+海藻糖组bcl-2表达量高于低钾右旋糖酐组、低钾右旋糖酐+二甲基亚砜组(P < 0.01)，但bax表达量低于低钾右旋糖酐组、低钾右旋糖酐+二甲基亚砜组(P < 0.01)；低钾右旋糖酐+二甲基亚砜组+海藻糖组bcl-2表达量最高，bax表达量最低，基本接近新鲜骨组织(P > 0.05)。结果提示海藻糖可能通过增强bcl-2基因、抑制bax基因的表达保护低温保存中的胸骨细胞活性，其与二甲基亚砜合用保护作用更好。

关键词: 海藻糖, 低温保存, 胸骨, bcl-2基因, bax基因

Abstract:

BACKGROUND: Studies have demonstrated that trehalose possesses protective effects on cyropreserved sternum. But the mechanism of action remains poorly understood.
OBJECTIVE: To investigate the effects of trehalose on bcl-2 and bax mRNA expression in cryopreserved sternum.
METHODS: Four groups of freshly prepared solution were used: low-potassium dextran (LPD), LPD + dimethyl sulfoxide (DMSO), LPD + trehalose, LPD + DMSO + trehalose. Rat sternum was cut and then immediately cryopreserved in the tubes containing each group of solution. Fresh rat sternum tissue and 4 groups of samples cryopreserved for 120 days were taken and bcl-2 and bax mRNA expression in fresh and cryopreserved sternum was detected using reverse transcription-polymerase chain reaction.
RESULTS AND CONCLUSION: bcl-2 mRNA expression in the LPD + trehalose group was significantly higher, but bax mRNA expression was significantly lower, than in the LPD, LPD + DMSO groups (both P < 0.01). LPD + DMSO + trehalose group showed highest bcl-2 mRNA expression and lowest bax mRNA expression, which were basically similar to fresh bone tissue   (P > 0.05). These findings indicate that trehalose may protect cell activity in cryopreserved sternum by enhancing bcl-2 mRNA expression and inhibiting bax mRNA expression, and trehalose together with DMSO shows better protective effects.

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