中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (15): 2773-2776.doi: 10.3969/j.issn.1673-8225.2011.15.027

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

缺血性糖尿病足部溃疡皮肤Vδ1T细胞胰岛素样生长因子1与诱生型一氧化氮合酶的表达

谢翠松1, 2，薛耀明1   

  1. 1南方医科大学附属南方医院内分泌代谢科，广东省广州市 510515
    2郴州市第一人民医院内分泌科，湖南省郴州市 423000
  • 收稿日期:2010-12-21 修回日期:2011-02-19 出版日期:2011-04-09 发布日期:2013-11-06
  • 通讯作者: 薛耀明，博士，主任医师，教授，博士生导师，南方医科大学附属南方医院内分泌代谢科，广东省广州市 510515 xie_h_r@yahoo.com.cn
  • 作者简介:谢翠松☆，女，1971年生，博士，主治医师，主要从事糖尿病足溃疡难愈合的机制研究。

Expression of insulin-like growth factor 1 and inducible nitric-oxide synthase in Vδ1T cells from ischemic diabtetic foot ulcer skins

Xie Cui-song1, 2, Xue Yao-ming1   

  1. 1Department of Endocrinology, Nanfang Hospital of Southern Medical University, Guangzhou  510515, Guangdong Province, China
    2Department of Endocrinology, First People’s Hospital of Chenzhou, Chenzhou  423000, Hunan Province, China
  • Received:2010-12-21 Revised:2011-02-19 Online:2011-04-09 Published:2013-11-06
  • Contact: Xue Yao-ming, Doctor, Chief physician, Professor, Doctoral supervisor, Department of Endocrinology, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China xie_h_r@yahoo.com.cn
  • About author:Xie Cui-song☆, Doctor, Attending physician, Department of Endocrinology, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China; Department of Endocrinology, First People’s Hospital of Chenzhou, Chenzhou 423000, Hunan Province, China

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摘要：

背景：皮肤Vδ1T细胞分泌的一系列细胞因子能促进伤口愈合，但与糖尿病足部皮肤组织溃疡难以愈合的关系尚未见报道。
目的：观察缺血性糖尿病患者足部溃疡皮肤Vδ1T细胞中胰岛素样生长因子1与诱生型一氧化氮合酶的表达。
方法：选取缺血性糖尿病足部溃疡患者及正常对照者足部全层皮肤，各10例。双酶标免疫荧光标记测量Vδ1T细胞及胰岛素样生长因子1、诱生型一氧化氮合酶蛋白表达。
结果与结论：缺血性糖尿病足部溃疡患者Vδ1T细胞中胰岛素样生长因子1蛋白表达较对照者明显减少(P < 0.01)；诱生型一氧化氮合酶蛋白表达明显增加((P  < 0.01)。提示Vδ1T细胞中胰岛素样生长因子1减少可能与缺血性糖尿病足部溃疡难以愈合有关，而胰岛素样生长因子1减少与溃疡皮肤Vδ1T细胞诱生型一氧化氮合酶表达增加，产生氧化应激有关。

关键词: 糖尿病足, V&delta, 1T细胞, 胰岛素样生长因子1, 诱生型一氧化氮合酶, 氧化应激

Abstract:

BACKGROUND: Skin Vδ1T cells can secrete a series of cell factors that can promote wound healing, but the association between cell factors and nonunion in ischemic diabetic foot ulcer skin are rare reported.
OBJECTIVE: To explore the association of expression of insulin-like growth factor-1 (IGF-1) and inducible nitric-oxide synthase (iNOS) in Vδ1T cells in ischemic diabetic foot ulcer skin.
METHODS: Ten patients with ischemic diabetic foot ulcer and 10 healthy people were selected. The protein expression of IGF-1 and iNOS was detected by Double-2 labeling immunofluorescence.
RESULTS AND CONCLUSION: The protein expression of IGF-1 in Vδ1T cells was decreased in diabetic foot group than that of control group (P < 0.01), but iNOS was increased than that of control group (P < 0.01), which indicated that the decrease of IGF-1 and the increase of iNOS in Vδ1 T cells correlated with ischemic diabetic foot ulcer healing.

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