中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9163-.doi: 10.3969/j.issn.1673-8225.2010.49.010

• 干细胞移植 • 上一篇    下一篇

磁共振评价关节腔内注射骨髓间充质干细胞治疗早期骨性关节炎

刘钊1，崔延安1，黄海青1，周钟珩1，庄 超2   

  1. 1江苏省中医院MR室，江苏省南京市  210029；  2南京医科大学附属南京第一医院骨科中心，江苏省南京市  210006
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 通讯作者: 通讯作者:崔延安，副主任医师，南京中药大学附属江苏省中医院CT、MR室，江苏省南京市210029 cya6902@sohu.com
  • 基金资助:

    江苏省自然科学基金面上项目(BK2009460)。

Intraarticular injection of bone marrow mesenchymal stem cells for treatment of early osteoarthritis detected by magnetic resonance

Liu Zhao1, Cui Yan-an1, Huang Hai-qing1, Zhou Zhong-heng1, Zhuang Chao2   

  1. 1 Department of MR, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing  210029, Jiangsu Province, China; 2 Orthopaedics Center, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing  210006, Jiangsu Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Cui Yan-an, Associate chief physician, Department of MR, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China cya6902@sohu.com
  • Supported by:

    the General Program of  Natural Science Foundation of Jiangsu Province, No.BK2009460*

PDF

403

被引次数

6

摘要：

背景：作者已对FS-3D-FISP序列诊断骨性关节炎做出研究评价，该序列对早期骨性关节炎的分级与病理有较好的一致性。
目的：用骨髓间充质干细胞治疗兔早期骨性关节炎，运用MR观察软骨厚度及信号的改变评价治疗效果。
方法：将新西兰大白兔随机分成对照组、骨性关节炎模型组和骨髓间充质干细胞组。骨髓间充质干细胞组在造模后第4周左膝关节腔内注射骨髓间充质干细胞。分别在术后第2周，1，2及3个月行膝关节核磁共振检查，每次每组随机选3只实验兔；扫描使用FS-3D-FISP序列测量膝关节软骨厚度, T2-FSE双回波序列测量T2值治疗前后改变,并取膝关节标本行病理观察进行Mankin’s评分，评价兔膝关节骨性关节炎治疗的效果。
结果与结论：与骨性关节炎组相比，骨髓间充质干细胞组MRI示关节软骨厚度接近正常，T2值减低，无关节腔积液。提示MR显示膝关节软骨厚度及信号的改变可用于评价骨性关节炎治疗前后的改变。

关键词: 骨性关节炎, 磁共振成像, 骨髓间充质干细胞, 膝关节, 腔内注射, 兔

Abstract:

BACKGROUND: Authors have assessed the FS-3D-FISP sequence for diagnosis of osteoarthritis. This sequence has a good consistency of the grade and pathology of early osteoarthritis.
OBJECTIVE: To observe the thickness and signal changes by magnetic resonance (MR) in the treatment of on early osteoarthritis in rabbits using bone marrow mesenchymal stem cells (BMSCs) transplantation.
METHODS: New Zealand white rabbits were randomly and equally divided into a control group, osteoarthritis group and BMSCs group. Injection of BMSCs was given to the left knee joint of rabbits in the BMSCs group on week 4 after arthritis induction. MR of knee joints was done at 2 weeks, 1, 2 and 3 months after the injection; three rabbits were randomly selected every time from each group. Using the sequence of FS-3D-FISP, the thickness of articular cartilage was measured and the T2-FSE double-eho sequences were utilized to measure the changes of T2 value after treatment. Pathohistological changes were observed as well as Mankin scores. The effect of the osteoarthritis in rabbit was evaluated.
RESULTS AND CONCLUSION: Compared with osteoarthritis group, the thickness of articular cartilage got close to normal; T2 value reduced, and without arthroedema shown in magnetic resonance imaging. Results indicated that the thickness and signal changes of the articular cartilage on MR can be used to evaluate the changes in osteoarthritis pre- and post-treatment.

中图分类号: