中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7715-7718.doi: 10.3969/j.issn.1673-8225.2010.41.028

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

细胞片工程在组织工程中的应用

邓林红，王  晟   

  1. 重庆大学生物工程学院生物微流变学与呼吸生物工程实验室，重庆市  400044
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 作者简介:邓林红☆，男，1960年生，四川省夹江县人，汉族，2000年于英国斯查克莱大学毕业，博士，教授，主要从事生物流变学与组织工程学研究。 denglh@cqu.edu.cn
  • 基金资助:

    课题受国家自然科学基金(30770529)资助。

Application of cell sheet engineering in tissue engineering

Deng Lin-hong, Wang Sheng   

  1. Laboratory of Biorheology and Respiratory Bioengineering, Bioengineering College of Chongqing University, Chongqing  400044, China
  • Online:2010-10-08 Published:2010-10-08
  • About author:Deng Lin-hong☆, Doctor, Professor, Loboratory of Biorheology and Respiratory Bioengineering, Bioengineering College of Chongqing University, Chongqing 400044, China denglh@cqu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30770529*

PDF

320

摘要：

背景：细胞片工程可以直接移植单层细胞片到母体组织，也可以通过叠加多层细胞片制得三维结构的组织应用于组织再生，可以弥补传统组织工程技术的不足。
目的：介绍细胞片工程在组织工程中的应用和研究进展。
方法：由第二作者用计算机检索中国期刊全文数据库(CNKI：2000/2010)和Medline(1990/2010)数据库，检索词分别为“组织工程，温敏性培养皿，细胞片工程”和“tissue engineering, temperature-responsive culture dishes, cell sheet engineering”，语言分别设定为中文和英文。从细胞片工程的简介、细胞片工程的应用及细胞片工程的局限之处与解决方法3个方面进行总结，对细胞片工程在组织工程中的应用与研究进展进行介绍。
结果与结论：共检索到82篇文章，按纳入和排除标准对文献进行筛选，共纳入40篇文章。结果表明，对于治疗受损或病变组织，器官移植并不是惟一的治疗方法，组织工程也是很有前景的治疗方法。生物可降解支架作为一种传统的组织工程的方法，在软骨，骨骼，血管等的修复上都取得了成功，但由于其应用范围受到了支架的生物相容性以及降解后副作用的制约，使其不能广泛推广应用。而新兴的细胞片工程由于无需使用支架，能够较好的应用于组织工程。细胞片工程已经在角膜修复，牙周膜修复和肺漏气修复等多个方面获得了成功。随着组织工程的不断发展，细胞片工程必将成为再生医学的重要工具。

关键词: 细胞片工程, 温敏性培养皿, 生物可降解支架, 修复, 组织工程

Abstract:

BACKGROUND: Cell sheet engineering allows for tissue regeneration by either direct transplantation of monolayer cell sheets to host tissues or the creation of three-dimensional structures via the layering of individual cell sheets.
OBJECTIVE: To introduce the application and its progress of cell sheet engineering in tissue engineering.
METHODS: Literature from Chinese Journal Full-Text Database (CNKI: 2000/2010) and Medline database (1990/2010) was searched by the first author using key words of “tissue engineering, temperature-responsive culture dishes, cell sheet engineering” in Chinese or English. The development of cell sheet engineering and its application in tissue engineering was reviewed from three aspects, that is, introduction of cell sheet engineering, application of cell sheet engineering, the shortcomings and solution of cell sheet engineering.
RESULTS AND CONCLUSION: Totally 82 papers were initial searched, and 40 papers were included in this paper. The results show that tissue engineering can be an alternative promising method for organ transplantation in the treatment to injured and pathological tissue. The traditional tissue engineering method, biodegradable scaffolds, have achieved success in some aspects of tissue engineering, such as cartilage repair, bone repair and blood vessel repair. But its application has been restricted by the shortcoming of scaffolds. However, cell sheet engineering can be a good supplement to tissue engineering by avoiding using scaffolds. Cell sheet engineering has been proved useful in cornea repair, periodontal repair, pulmonary air leakage and so on. With the development of tissue engineering, cell sheet engineering should be an important tool for regenerative medicine.

中图分类号: