中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (36): 6772-6775.doi: 10.3969/j.issn.1673-8225.2010.36.027

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

造血干细胞向淋巴系祖细胞增殖分化过程中HOXB4基因的表达

邹  艳，翟雪松，陈红英   

  1. 泸州医学院附属医院儿科，四川省泸州市  646000
  • 出版日期:2010-09-03 发布日期:2010-09-03
  • 通讯作者: 刘文君，主任医师，教授，儿科主任，博士，硕士生导师，泸州医学院附属医院儿科，四川省泸州市 646000 lwjlyfy@yahoo. com.cn
  • 作者简介:邹艳★，女，1974年生，四川省泸州市人，汉族，2009年泸州医学院毕业，硕士，主治医师，主要从事小儿血液病的研究。 zouyan1212@ 163.com
  • 基金资助:

    四川省教育厅重点科研项目(2004A058)

HOXB4 gene expression during differentiation and proliferation of hematopoietic stem cells into lymphocyte progenitor cells

Zou Yan, Zhai Xue-song, Chen Hong-ying   

  1. Department of Pediatrics, Hospital Affiliated to Luzhou Medical College, Luzhou  646000, Sichuan Province, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Liu Wen-jun, Chief physician, Professor, Doctor, Master’s supervisor, Department of Pediatrics, Hospital Affiliated to Luzhou Medical College, Luzhou 646000, Sichuan Province, China lwjlyfy@yahoo.com. cn
  • About author:Zou Yan★, Master, Attending physician, Department of Pediatrics, Hospital Affiliated to Luzhou Medical College, Luzhou 646000, Sichuan Province, China zouyan1212@163. com
  • Supported by:

    the Key Scientific Research Program of Department of Education of Sichuan Province, No. 2004A058*

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被引次数

1

摘要：

背景：HOXB4基因不仅能促进造血干细胞的扩增及其功能的活化与表达，而且体内试验表明它不会诱发白血病。因此更好地研究HOXB4在造血细胞增殖分化中的变化及作用对进一步研究造血干细胞的扩增可以提供更多的理论基础。
目的：观察人类脐血造血干细胞向淋巴系祖细胞增殖分化过程中HOXB4基因表达的情况及全反式维甲酸对HOXB4基因表达的影响。
方法：将培养的淋巴系造血祖细胞按干预方式不同分为2组，全反式维甲酸组：在培养体系中加入全反式维甲酸，终浓度6×10-8 mol/L。正常组：不加全反式维甲酸，代之以等量的1640培养液。观察人类脐血造血干细胞经植物血凝素诱导后，在培养第3，7，12天的淋巴细胞集落形成单位生成情况。采用实时荧光定量PCR技术检测脐血造血干细胞向淋巴系祖细胞增殖分化过程中HOXB4基因的表达水平。
结果与结论：人脐血造血干细胞向淋巴系祖细胞增殖分化过程中，HOXB4基因呈规律的表达。随培养时间推移，正常组和全反式维甲酸组HOXB4基因的表达均逐渐降低。与正常组比较，全反式维甲酸可上调HOXB4基因的表达。

关键词: 造血干细胞, 淋巴系祖细胞, HOXB4基因, 全反式维甲酸, 实时荧光定量PCR

Abstract:

BACKGROUND: Homeobox B4 (HOXB4) gene not only promotes amplification of hematopoietic stem cells (HSCs) and activation and expression of its function, but also in vivo test suggested that HOXB4 cannot induce leukemia. Therefore, to study HOXB4 changes and effects in proliferation and differentiation of HSCs provide theoretical basis for HSCs amplification.
OBJECTIVE: To observe the expression of HOXB4 gene in the proliferation and differentiation of HSCs into lymphocyte progenitor cells, and analyze the effects of all-train retinoic acid (ATRA) on HOXB4 gene.
METHODS: Lymphocyte hematopoietic progenitor cells were divided into two groups. In ATRA group, 6×10-8 mol/L ATRA was added in the culture system. In normal group, an equal volume of 1640 medium was added in the culture system. The colony growth of colony forming unit-lymphocyte in the differentiation progress of the HSCs to lymphocyte progenitor cells induced by phytohemagglutinin was observed on days 3, 7 and 12 following culture. Expression of HOXB4 gene in the proliferation and differentiation of the HSCs into lymphocyte progenitor cells in vitro was detected using real-time fluorescence quantitive polymerase chain reaction.
RESULTS AND CONCLUSION: HOXB4 gene has a regulatory function in the differentiation progress of the HSCs into lymphocyte progenitor cells. The expression of HOX B4 gene was diminished gradually in normal and ATRA groups over time. Compared with the normal group, HOXB4 gene was up-regulated in the ATRA group.

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