中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (34): 6305-6307.doi: 10.3969/j.issn.1673-8225.2010.34.009

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

骨膜包绕松质骨粒及骨形态发生蛋白纤维蛋白胶复合体重建月骨

胡成栋1，孟  钊2，邵新中1   

  1. 1河北医科大学第三医院手外科，河北省石家庄市   050051；2河北省儿童医院骨科，河北省石家庄市050000
  • 出版日期:2010-08-20 发布日期:2010-08-20
  • 通讯作者: 孟钊，主任医师，教授，河北省儿童医院骨科，河北省石家庄市 050000
  • 作者简介:胡成栋☆，男，1973年生，河北省邯郸市人，汉族，2005年解放军总医院毕业，博士，副主任医师，目前工作于河北省邯郸市中心医院骨二科，主要从事骨与关节损伤修复临床与相关基础研究。 hcd111111@163.com

Compound bodies of periostcum wrappping grains of cancellous bone and fibrin sealant combined with bone morphogenetic protein for semilunar bone reconstruction

Hu Cheng-dong1, Meng Zhao2, Shao Xin-zhong1   

  1. 1 Department of Hand Surgery, Third Hospital, Hebei Medical University, Shijiazhuang  050051, Hebei Province, China; 2 Department of Orthopaedics, Hebei Provincial Children’s Hospital, Shijiazhuang  050000, Hebei Province, China
  • Online:2010-08-20 Published:2010-08-20
  • Contact: Meng Zhao, Chief physician, Professor, Department of Orthopaedics, Hebei Provincial Children’s Hospital, Shijiazhuang 050000, Hebei Province, China
  • About author:Hu Cheng-dong☆, Doctor, Associate chief physician, Department of Hand Surgery, Third Hospital, Hebei Medical University, Shijiazhuang 050051, Hebei Province, China hcd111111@163.com

PDF

442

被引次数

3

摘要：

背景：月骨缺血性坏死III、IV期患者，需行月骨切除，而如何选用适宜的替代物填充月骨摘除后所留的间隙，成为治疗此病的关键。
目的：评价骨膜包绕松质骨粒及骨形态发生蛋白纤维蛋白胶复合体在体内成骨成软骨能力，探索重建月骨治疗月骨缺血性坏死的可行性。
方法：将日本大耳白兔分为3组：分别于兔膝关节髌上囊置入骨膜包绕松质骨粒及骨形态发生蛋白纤维蛋白胶复合体，骨膜包绕松质骨粒，骨膜包绕肌腱。术后2，4，8，12，16，24周取标本进行大体、放射学、光镜及电镜检查，并对复合体CT值进行统计学分析。
结果与结论： 复合体组复合体2周即有新生骨出现，8周形成外周为完整骨包壳中心为骨髓细胞的类圆形骨性团块，24周复合体无退变吸收，骨膜包绕松质骨粒组复合体4周有新生骨与软骨形成，8周形成较成熟骨小梁，24周形成骨性团块；骨膜包绕肌腱组复合体24周内无骨团块形成。术后复合体组，骨膜包绕松质骨粒组与骨膜包绕肌腱组复合体CT值进行比较，差异均有显著性意义。结果提示，骨膜包绕松质骨粒及骨形态发生蛋白纤维蛋白胶复合体组复合体成骨时间早、成骨完全，并具有适宜的可塑性等优点。

关键词: 骨软骨炎, 骨膜, 骨形态发生蛋白, 纤维蛋白胶, 月骨, 兔

Abstract:

BACKGROUND: The patients with Kienbock’s disease in stages III and IV, whose lunatum needs to be cut, and how to select eligible surrogate to fill the interspace was the key point to cure Kienbock’s disease.
OBJECTIVE: To evaluate the ability to form bone and cartilage of autologous periosteum wrapping grains of cancellous bone and fibrin sealant combined with bone morphogenetic protein (BMP) compound, in order to explore the feasibility of reconstructing lunate bone for treating Kienbock’s disease.
METHODS: Japanese big ear rabbits were divided into three groups: periostum wrapping grains of cancellous bone and fibrin sealant combined with BMP were respectively implanted in rabbit knee joint suprapatellar bursa; grains of cancellous bone were wrapped with bone periostum; tendon was wrapped with periostum. The samples were observed at 2, 4, 8, 12, 16 and 24 weeks after operation using general examination, radiological examination, optical and electron microscope tests. CT values of compound bodies were statistically analyzed. 
RESULTS AND CONCLUSION: Newly formed bone could be found at 2 weeks in compound bodies group. Rounded bony body with complete bone shell and bone marrow cells were formed in the implanted composite at 8 weeks. No degenerative changes were observed up to 24 weeks. In periosteum wrapping grains of cancellous bone group, newly formed bone and cartilage were found at 4 weeks. Mature bone trabecula was formed at 8 weeks. Rounded bony body was formed at 24 weeks. In periosteum wrapping tendon group, the composite did not form bony body until 24 weeks. Significant differences in CT value among all groups were found. Results have indicated that in compound bodies group, bony tissue forms completely, ossification occurs early, with good plasticity.

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