中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (33): 6258-6261.doi: 10.3969/j.issn.1673-8225.2010.33.043

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

肌腱细胞转化生长因子β及其受体表达：6-磷酸果糖的抑制效应

申延清1，夏长所1，马爱国2   

  1. 1青岛大学医学院附属医院，山东省青岛市  266003；2青岛大学医学院，山东省青岛市  266003
  • 出版日期:2010-08-13 发布日期:2010-08-13
  • 通讯作者: 马爱国，教授，博士导师，青岛大学医学院，山东省青岛市 266003
  • 作者简介:申延清★，女，1972年生，山东省青岛市人，汉族，青岛大学医学院在读硕士，讲师，主要从事营养与食品卫生学研究。

Inhibition effect of mannose-6-phosphate on expression of transforming growth factor beta and its receptor in flexor tendon cells

Shen Yan-qing1, Xia Chang-suo1, Ma Ai-guo2   

  1. 1 Affiliated Hospital of Medical College, Qingdao University, Qingdao   266003, Shandong Province, China; 2 Medical College, Qingdao University, Qingdao   266003, Shandong Province, China 
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Ma Ai-guo, Professor, Doctoral supervisor, Medical College, Qingdao University, Qingdao 266003, Shandong Province, China yzhsyq@163.com
  • About author:Shen Yan-qing★, Studying for master’s degree, Lecturer, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China

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摘要：

背景：转化生长因子β在肌腱愈合与粘连形成中具有重要的作用，抑制转化生长因子β及其受体表达可起到一定的防止肌腱术后粘连作用。
目的：探讨转化生长因子β天然抑制剂6-磷酸果糖对兔屈趾肌腱腱鞘、腱外膜和腱内膜细胞转化生长因子β及其受体的影响。
方法：取兔屈趾肌腱分离培养腱鞘、腱外膜和腱内膜细胞。3种细胞分别加入6-磷酸果糖(实验组)或不加6-磷酸果糖(对照组)培养。采用酶联免疫吸附实验定量检测转化生长因子β及其受体的表达，原位杂交和免疫组织化学观察观测转化生长因子β1的表达。 
结果与结论：实验组细胞转化生长因子β及其受体的表达均较对照组下降(P < 0.05)。实验组3种细胞阳性转化生长因子β1 mRNA表达率及细胞内转化生长因子β1 mRNA表达强度均较对照组明显降低(P < 0.05)。免疫组化显示加入6-磷酸果糖培养后，3种细胞转化生长因子β1表达均明显降低。

关键词: 肌腱细胞, 6-磷酸果糖, 转化生长因子&beta, 转化生长因子&beta, 受体, 兔

Abstract:

BACKGROUND: Transforming growth factor beta (TGF-β) has an important role in tendon healing and adhesion formation. Inhibiting TGF-β and its receptor expression may prevent adhesions after tendon open.
OBJECTIVE: To study the effects of mannose-6-phosphate, a natural inhibitor of TGF-β, on TGF-β and its receptor production in tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes of rabbit flexor toes.
METHODS: Tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes were isolated from rabbit flexor tendon and cultured separately. All these cells were divided into 2 groups at random, experiment group supplemented with mannose-6-phosphate and control group without mannose-6-phosphate. The expression of TGF-β and TGF-β receptor was quantified with enzyme-linked immunosorbent assay. The expression of TGF-β1 was also assessed with in situ hybridization and immunohistochemistry.
RESULTS AND CONCLUSION: The expression of TGF-β and TGF-β receptor in experiment group was significantly lower than that in control group (P < 0.05). In experimental group, the positive expression of TGF-β1 mRNA and the expression level of intracellular TGF-β1 mRNA in all tendon cells demonstrated significantly lower than those in the control group (P < 0.05). Immunohistochemical staining showed expression of TGF-β1 were significantly lower in all three types of tendon cell cultured with mannose-6-phosphate.

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