中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (33): 6206-6209.doi: 10.3969/j.issn.1673-8225.2010.33.031

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

鼠类膝骨关节炎模型的评价

周景辉，吴耀持，李石胜，朱伟民，张圣宏   

  1. 上海交通大学附属第六人民医院针推伤科，上海市 200233
  • 出版日期:2010-08-13 发布日期:2010-08-13
  • 通讯作者: 吴耀持，主任医师，博士生导师，上海交通大学附属第六人民医院针推伤科，上海市 200233 wuyaochi@online.sh.cn
  • 作者简介:周景辉★，男，1982生，福建省武夷山市人，汉族，2008年上海中医药大学毕业，硕士，医师，主要从事骨关节病的相关研究。 zhoujinghui520@163.com
  • 基金资助:

    课题由上海交通大学附属第六人民医院院级课题(院内-116)赞助。

Evaluation on murine models of knee osteoarthritis

Zhou Jing-hui, Wu Yao-chi, Li Shi-sheng, Zhu Wei-min, Zhang Sheng-hong   

  1.  Department of Acupuncture and Massage, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai   200233, China
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Wu Yao-chi, Chief physician, Doctoral supervisor, Department of Acupuncture and Massage, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China wuyaochi@online.sh.cn
  • About author:Zhou Jing-hui★, Master, Physician, Department of Acupuncture and Massage, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China zhoujinghui520@163.com
  • Supported by:

    the Foundation of Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, No. Yuannei-116*

PDF

1064

被引次数

16

摘要：

背景：探索膝骨关节炎动物模型建立的方法，是研究其病因和治疗方法的重要课题之一。常用于膝骨关节炎模型的实验动物为鼠类，模型制备的方法也各有不同，根据实验的需要可以选用不同的造模方式。
目的：探讨鼠类膝骨关节炎动物模型的建立方法，并对模型的制备做出评价。
方法：应用计算机检索CNKI 期刊全文数据库和PubMed数据库(1990-01/2009-12)与膝骨关节炎鼠类模型有关的文章，检索词为“膝骨关节炎；模型；鼠；评价”和“knee osteoarthritis；model；rat；valuation”，语言分别设定为中文和英文。对资料进行初审，剔除临床观察，查找全文，并查看每篇文献后的引文。纳入实验研究，实验动物为鼠类的文章，排除重复研究及不典型报道。
结果与结论：共纳入39 篇关于鼠类在膝关节骨性关节炎实验的研究文章，25篇中文文献和14篇外文文献符合纳入标准。经分析得出结论：通过鼠类骨内高压造成骨性关节炎模型使实验动物膝关节稳定性好，造模方法操作简单、创伤小，基本符合临床上膝骨关节炎的发生机制，故获得的膝骨关节炎模型较为可靠。根据实际情况(如经费预算、实验条件等)可灵活选用更好的造模方法。

关键词: 膝骨关节炎, 鼠, 模型, 实验方法, 综述文献

Abstract:

BACKGROUND: To explore the method of establishing animal models of knee osteoarthritis is a key topic of studying its etiopathogenisis and therapeutic method. Murine is commonly-used experimental animals for knee osteoarthritis model establishment. Model establishment method is various. According to the aims of experiments, different ways of establishing models were chosen.
OBJECTIVE: To analyze establishment methods of animal models of murine knee osteoarthritis, and to evaluate model preparation.
METHODS: CNKI full-text database and PubMed database were searched for articles about murine model for knee osteoarthritis published from January 1990 to December 2009 with the key words of “knee osteoarthritis, model, rat, valuation”. All data were primarily screened to exclude clinical observation and to look up full-texts. Moreover, references of each literature were checked. Experimental study (experimental animal was murine) was included. Repetitive studies and untypical study reports were excluded.
RESULTS AND CONCLUSION: Totally 39 articles on murine experimental researches of knee osteoarthritis were collected and 25 in Chinese and 14 in English met the inclusive criteria. We draw the conclusion that murine intraosseous hypertension-induced osteoarthritis models showed good stability of animal knee joint; the methods of creating models are simple, with small trauma, which is consistent with mechanisms of knee osteoarthritis in clinic. Thus, the obtained models of knee osteoarthritis are reliable. A better model establishment method can be selected according to practical conditions (such as expense budget, experimental condition).

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