中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (33): 6138-6142.doi: 10.3969/j.issn.1673-8225.2010.33.014

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

人浆细胞瘤Balb/c-nu-hu模型的建立

黎宁君，张  宏，冯一中，王盼君，傅晋翔   

  1. 苏州大学附属第二医院血液科，江苏省苏州市 215004
  • 出版日期:2010-08-13 发布日期:2010-08-13
  • 通讯作者: 傅晋翔，博士，主任医师，教授，苏州大学附属第二医院血液科，江苏省苏州市 215004 uufixly@public1.szjs.cn
  • 作者简介:黎宁君★，女，1982年生，湖南省娄底市人，汉族，苏州大学在读硕士，主要从事造血调控方面的研究。 liningjun0714@yahoo.com.cn
  • 基金资助:

    中核集团“辐射损伤救治等核应急相关技术研究”项目资助。

Establishment of a Balb/c-nu-hu model of human plasmacytoma

Li Ning-jun, Zhang Hong, Feng Yi-zhong, Wang Pan-jun, Fu Jin-xiang   

  1. Department of Hematology, Second Affiliated Hospital, Soochow University, Suzhou  215004, Jiangsu Province, China
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Fu Jin-xiang, Doctor, Chief physician, Professor, Department of Hematology, Second Affiliated Hospital, Soochow University, Suzhou 215004, Jiangsu Province, China uufixly@public1.szjs.cn
  • About author:Li Ning-jun★, Studying for master’s degree, Department of Hematology, Second Affiliated Hospital, Soochow University, Suzhou 215004, Jiangsu Province, China liningjun0714@yahoo.com.cn
  • Supported by:

    Fund of China National Nuclear Corporation*

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摘要：

背景：建立相关动物模型是研究多发性骨髓瘤病理生理机制及药物治疗的重要方法。
目的：为研究多发性骨髓瘤细胞在人胎骨微环境内的生长特性，建立新的Balb/c-nu-hu人鼠嵌合的浆细胞瘤动物模型。
方法：取孕16周人女性胎儿1 cm左右胎骨植入Balb/c-nu裸鼠皮下，建立新的Balb/c-nu-hu鼠-人嵌合体；胎骨植入3周后将白细胞介素6依赖的人多发性骨髓瘤细胞株XG-7悬液注入胎骨内建立人浆细胞瘤Balb/c-nu-hu模型；观察裸鼠及肿瘤的生长情况。40 d后，取胎骨、肿瘤及裸鼠重要组织行苏木精-伊红染色及抗人CD34、CD59、CD138和VEGF免疫组织化学染色；X射线片前后对比检查模型裸鼠体内胎骨骨密度的变化。
结果与结论：胎骨表面及胎骨内见新生血管生成，且具有高度活性；XG-7细胞株能在植入人胎骨的裸鼠体内生长、浸润，形成髓外肿瘤，具有多种与浆细胞瘤相似的病理学特征，其中免疫组织化学结果显示CD138、CD59阳性说明其具有浆细胞表面标记，血管CD34阳性表明其为人源性；模型裸鼠出现恶病质，终末人骨髓瘤细胞浸润播散至鼠外周血及淋巴结，X射线片显示胎骨出现骨破坏。说明Balb/c-nu-hu鼠-人嵌合体模型可作为一种建立肿瘤模型可靠的基础动物载体，利用Balb/c-nu-hu鼠-人嵌合体可以成功建立人浆细胞瘤模型。

关键词: 浆细胞瘤, Balb/c-nu裸鼠, 模型, 胎骨, 细胞株

Abstract:

BACKGROUND: Establishment of a plasmacytoma model plays an important role in study of the physiological mechanism and medication for multiple myeloma.
OBJECTIVE: To establish a novel plasmacytoma model of Balb/c-nu-hu for further investigation of the growth of multiple myeloma (MM) cell line in human fetal bone.
METHODS: Subcutaneously transplantation of 1-cm long segment of 16 weeks old human fetal thigh or tibia bone into Balb/c-nu mice to develop a novel chimeric model based on the Balb/c-nu-hu, 3 weeks later inoculated the interleukin-6 (IL-6)-dependent human MM cell line XG-7 into the human fetal bone. Human fetal bone implanted as well as extramedullary macro-tumors and important organs of the mouse model respectively stained by hematoxylin-eosin staining and monoclonal mouse anti-human CD34, CD59, CD138 and VEGF followed by morphological examination. X-ray film was used to examine the changes of bone density of fetal bone in vivo.
RESULTS AND CONCLUSION: New vessels could be observed on the surface and in fetal bones, with highly activity. The MM cell line XG-7 could grow, infiltrate and migrate in nude mice and formed extramedullary tumor, which exhibited similar pathological features to plasmacytoma. The positive expression of CD138 and CD59 demonstrated that the tumor had surface marker of plasma cell, and the positive CD34 showed it was derived from human. X-ray film showed the resorption of the human bones. The model was characterized by cachexia, terminal scatter and migration of myeloma cells. The Balb/c-nu-hu chimeric model is a novel vector for researching hominine hematopoiesis and bone based on animal. The MM cell can growth successfully based on the Balb/c-nu-hu chimera.

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