中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 5228-5232.doi: 10.3969/j.issn.1673-8225.2010.28.023

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

银杏黄酮苷元干预兔颈总动脉损伤后内膜增生及血凝素样氧化低密度脂蛋白受体1的表达

何  艳，谢清平，吴立荣，刘兴德，方  颖   

  1. 贵阳医学院附属医院心内科，贵州省贵阳市 550004
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 作者简介: 何 艳☆，女，1972年生，湖南省澧县人，汉族，博士，2005年北京大学毕业，副教授，主要从事动脉粥样硬化基础和临床研究。 heyanphysician@gmail.com
  • 基金资助:

    本课题获贵州省优秀科技教育人才省长专项资金项目[黔省专合字(2005)225号]资助。

Effect of ginkgo flavone aglycone intervention on intimal proliferation and lectin-like oxidized low-density lipoprotein receptor-1 expression in rabbits following carotid balloon injury  

He Yan, Xie Qing-ping, Wu Li-rong, Liu Xing-de, Fang Ying   

  1. Cardiac Internal Medicine, Affiliated Hospital of Guiyang Medical College, Guiyang  550004, Guizhou Province, China
  • Online:2010-07-09 Published:2010-07-09
  • About author:He Yan☆, Doctor, Associate professor, Department of Cardiac Internal Medicine, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China heyanphysician@gmail.com
  • Supported by:

    the Special Fund of the Governor of Guizhou Province for Excellent Scientific, Technological and Educational Talents, No. (2005)225*

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491

被引次数

3

摘要：

背景：银杏叶提取物中的黄酮苷元具有较强的抗氧化活性，课题组前期实验证实可抑制氧化低密度脂蛋白诱导内皮细胞表达血凝素样氧化低密度脂蛋白受体1。
目的：进一步探讨银杏黄酮苷元对兔颈总动脉内皮损伤后内膜增生和血凝素样氧化低密度脂蛋白受体1表达的影响。
方法：将雄性新西兰大白兔随机分为对照组、假手术组、模型组和治疗组。对照组予普通饲料，其余各组予高脂饮食，假手术组仅作颈外动脉结扎，模型组和治疗组均球囊损伤右颈总动脉，治疗组损伤后用银杏黄酮苷元灌胃。4周后检测各组血脂水平，观察各组右颈总动脉形态，用免疫组织化学法和RT-PCR检测血凝素样氧化低密度脂蛋白受体1蛋白和mRNA表达。
结果与结论：模型组术后4周内膜增生明显，有粥样斑块形成，血凝素样氧化低密度脂蛋白受体1表达明显增加(P < 0.05)。治疗组银杏黄酮苷元灌胃4周后内膜增生较轻，内膜面积，血凝素样氧化低密度脂蛋白受体1阳性细胞数和其基因表达水平均低于模型组(P < 0.05)，但血脂与模型组比较无差异。提示银杏黄酮苷元可减轻新生内膜增生及动脉粥样硬化病粥样斑块的形成，这种作用可能与抑制兔颈总动脉内皮损伤后血凝素样氧化低密度脂蛋白受体1的表达有关。

关键词: 银杏黄酮苷元, 内膜增生, 血凝素样氧化低密度脂蛋白受体1, 颈总动脉, 动脉粥样硬化, 组织构建 ,  ,  ,  

Abstract:

BACKGROUND: Flavone aglycone, a component of ginkgo biloba extract, has strong antioxidant activity. Preliminary studies have demonstrated that ginkgo flavone aglycone (GFA) can inhibit lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) expression induced by oxidized low density lipoprotein.
OBJECTIVE: To investigate the effect of GFA on the intimal proliferation and LOX-1 expression in rabbits after carotid endothelial injury.
METHODS: New Zealand male rabbits were randomly divided into the control, sham surgery, model, and treatment groups. All rabbits were fed with high cholesterol chow except that in the control group. Rabbits in the sham surgery group underwent surgery without endothelium injury, those in the model and treatment groups underwent carotid balloon injury. GFA was administered daily to the treatment group by gavages. Serum lipids in 4 groups were detected at 4 weeks after operation. The carotid artery segments were harvested for histomorphometry observation. LOX-1 levels were measured though SP immunohistochemistry assay and RT-PCR.
RESULTS AND CONCLUSION: Neointimal proliferation and atherosclerosis was found in carotid artery of the model group at 4 weeks after operation, and the expression of LOX-1 was notably increased (P < 0.05). In the treatment group, the intimal area, intimal/medial area ratio and the LOX-1 expression were smaller than those of the model group (P < 0.01), but the serum lipids levels had no difference. GFA can inhibit neointimal proliferation and atherosclerosis progress, which may be related to down-regulated expression of LOX-1 after carotid balloon injury.

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