中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 5224-5227.doi: 10.3969/j.issn.1673-8225.2010.28.022

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

康肤霜对大鼠放射性皮肤损伤创面愈合的影响

龚震宇1，童亚林1，万友华2，杨润生3，苏晓玲3   

  1. 解放军181医院，1烧伤整形科，2病理科，3动物实验中心，广西壮族自治区桂林市 541002
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 通讯作者: 童亚林，主任医师，教授，解放军181医院烧伤整形科，广西壮族自治区桂林市 541002
  • 作者简介:龚震宇★，男，1976年生，江西省南昌市人，汉族，2005年解放军第一军医大学毕业，硕士，主治医师，主要从事创面修复的临床及基础研究。 gongzhenyu76@126.com.
  • 基金资助:

    广西自然科学基金资助项目(2060203-255)，康肤霜促进难愈性创面的基础及临床研究。

Effects of Kangfushuang on skin radiation injury healing in rats 

Gong Zhen-yu1, Tong Ya-lin1, Wan You-hua2, Yang Run-sheng3, Su Xiao-ling3   

  1. 1 Department of Burns and Plastic Surgery, 2 Department of Pathology, 3 Experimental Animal Center, No. 181 Hospital of Chinese PLA, Guilin  541002, Guangxi Zhuang Autonomous Region, China
  • Online:2010-07-09 Published:2010-07-09
  • Contact: Tong Ya-lin, Chief physician, Professor, Department of Burns and Plastic Surgery, No. 181 Hospital of Chinese PLA, Guilin 541002, Guangxi Zhuang Autonomous Region, China
  • About author:Gong Zhen-yu★, Master, Attending physician, Department of Burns and Plastic Surgery, No. 181 Hospital of Chinese PLA, Guilin 541002, Guangxi Zhuang Autonomous Region, China gongzhenyu76@126.com
  • Supported by:

    Guangxi Natural Science Foundation, No. 2060203-255*

PDF

360

被引次数

8

摘要：

背景：放疗中引起的放射性皮肤损伤，常使用重组人表皮生长因子和聚维酮碘治疗，但两者均有一定缺陷。而康肤霜能促进细胞分裂增殖，具有一定的抗菌消炎作用，可能对放射性皮肤损伤有很好的疗效。
目的：观察康肤霜对大鼠放射性皮肤损伤创面愈合的影响。
方法：高能X射线照射Wistar大鼠臀部皮肤建立深Ⅱ度皮肤烧伤创面模型。照射后12 d创面出现后，将损伤动物随机分为康肤霜组，重组人表皮生长因子组和聚维酮碘组。各组每日将药物均匀涂抹在创面，直至创面取材或创面愈合。观察创面愈合率及愈合时间。各组动物分别于创面治疗后7，14，21 d取创面组织，观察病理组织学变化、创面组织增殖细胞核抗原的表达，以标记指数法测定增殖细胞核抗原阳性细胞数，评估组织创面的修复情况。
结果与结论：康肤霜组与重组人表皮生长因子组创面愈合时间、愈合率均优于聚维酮碘组(P < 0.01)，但两组间无显著差异   (P > 0.05)且两组创面组织标本中增殖细胞核抗原表达量也明显高于聚维酮碘组(P < 0.01)，但其两组间比较差异无显著性意义(P > 0.05)。说明康肤霜能减轻创面早期的炎症反应，促进创面的再上皮化和表皮各层的分化，加速大鼠放射性皮肤损伤创面的愈合。

关键词: 放射损伤, 创面愈合, 增殖细胞核抗原, 表皮生长因子, 大鼠, 皮肤组织工程

Abstract:

BACKGROUND: Recombinant human epidermal growth factor (rhEGF) and povidone iodine are common used for treating radiotherapy-induced radiation skin injury, but both of the two methods have drawbacks. Kangfushuang (KFS) can promote cell proliferation and possesses antibacterial and antiphlogistic effects, which may be benefit to skin healing after radiation injury.
OBJECTIVE: To observe effects of KFS on skin radiation injury healing in rats. 
METHODS: A Wistar rat model with deep II degree burn was prepared by locally irradiating with high energy X radiation of accelerator. At 12 days after radiation, the animals with skin injury were randomly divided into KFS, rhEGF and povidone iodine groups, and treated by daily smear of drugs. The healing rate and time were observed in all the groups. The wound tissues were harvested at days 7, 14, 21 after treatment, the pathohistological changes of wound tissues, the expression of proliferating cell nuclear antigen (PCNA) were observed. The PCNA positive cells were measured by labeling index to evaluate the tissue repair in wound healing.
RESULTS AND CONCLUSION: The healing time and healing rates of the KFS group and rhEGF group were significantly better than that of the povidone iodine group (P < 0.01), but there was no significant difference between the KFS group and rhEGF group (P > 0.05). The expression of PCNA in the KFS group and rhEGF group were significantly superior to that of the povidone iodine group (P < 0.01), but there was no significant difference between the KFS group and rhEGF group (P > 0.05). The results showed that KFS can relief the early inflammatory reaction, enhance wound re-epithelization and differentiation of epidermal layers, and accelerate skin healing after radiation injury in rats.

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