中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (24): 4449-4453.doi: 10.3969/j.issn.1673-8225.2010.24.018

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

β2-肾上腺素能受体过表达后成年犬心肌细胞收缩功能的改变及机制

单  雨1，宫海滨1，2，3，王  雷2，王秀力2，吕  茜2，张清林2，刘  颖2，王  洁2，庞  慧2，苏景力1，王振全2   

  1. 1东南大学医学院，江苏省南京市  210009；2徐州市心血管病研究所，江苏省徐州市  221009；3徐州市中心医院心内科，江苏省徐州市 221009
  • 出版日期:2010-06-11 发布日期:2010-06-11
  • 通讯作者: 宫海滨，博士，教授，硕士生导师，东南大学医学院，江苏省南京市 210009；徐州市心血管病研究所，江苏省徐州市 221009；徐州市中心医院心内科，江苏省徐州市 221009 ghbxzh@yahoo.com.cn
  • 作者简介:单 雨★，男，1980年生，安徽省滁州市人，汉族，东南大学医学院在读硕士，主要从事细胞水平的转基因实验研究。
  • 基金资助:

    国家自然科学基金资助项目(30572073)《转beta2-肾上腺素受体基因治疗心力衰竭的应用基础研究》；江苏省自然科学基金项目(BK2005428)《转人beta2-肾上腺素受体基因治疗心力衰竭的应用基础研究》；江苏省医学重点人才研究基金(RC2007024)《β2-肾上腺素受体基因治疗心力衰竭的作用及机制探讨》；江苏省卫生厅重大科研课题资助项目(K200402)《人β2AR基因治疗心力衰竭的临床基础研究》；徐州市社会发展项目(XM09B073)《过表达β2-肾上腺素受体基因对心衰大鼠心肌细胞收缩功能影响的分子信号机制的研究》。

Changes in contractile function of cardiocytes following overexpressing beta 2 adrenergic receptor in canines and the underlying mechanisms

Shan Yu1, Gong Hai-bin 1，2,3, Wang Lei2, Wang Xiu-li2, Lü Qian2, Zhang Qing-lin2, Liu Ying2, Wang Jie2, Pang Hui2, Su Jing-li1, Wang Zhen-quan2   

  1. 1 edical College of Southeast University, Nanjing  210009, Jiangsu Province, China; 2 Xuzhou Cardiovascular Disease Institute, Xuzhou  221009, Jiangsu Province, China; 3 Department of Cardiology, Xuzhou Central Hospital, Xuzhou  221009, Jiangsu Province, China
  • Online:2010-06-11 Published:2010-06-11
  • Contact: Gong Hai-bin, Doctor, Professor, Master’s supervisor, Medical College of Southeast University, Nanjing 210009, Jiangsu Province, China; Xuzhou Cardiovascular Disease Institute, Xuzhou 221009, Jiangsu Province, China; Department of Cardiology, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu Province, China ghbxzh@yahoo.com.cn
  • About author:Shan Yu★, Studying for master’s degree, Medical College of Southeast University, Nanjing 210009, Jiangsu Province, China shanyuat@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30572073*; the Natural Science Foundation of Jiangsu Province, No. BK2005428*; the Outstanding Medical Academic Leader Program of Jiangsu Province (135 Program), No. RC2007024*?; the Major Research Projects of Health Department of Jiangsu Province, No. K200402*?; the Social Development Project of Xuzhou, No. XM09B073*

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摘要：

背景：在转基因小鼠、大鼠等小动物实验中发现，心肌细胞特异性过表达β -肾上腺素受体可以改善其收缩功能，但目前尚缺乏在大型哺乳动物进行的有关实验报道。
目的：探讨犬心肌细胞过表达β2-肾上腺素受体后心肌细胞收缩功能的改变及其可能机制。
方法：采用胶原酶消化法分离培养犬心肌细胞，用携带β2-肾上腺素受体目的基因的重组腺病毒转染心肌细胞。转染48 h后观察心肌细胞β2-肾上腺素受体蛋白表达及在基础状态和最大收缩状态下(以异丙肾上腺素10-6 mol/L刺激)心肌细胞cAMP水平及收缩功能的变化。
结果与结论：转染β2-肾上腺素受体的心肌细胞β2-肾上腺素受体蛋白表达及胞内cAMP水平明显增多(P < 0.05)，基础状态下收缩功能明显增强，但不改变最大收缩幅度百分比。说明β2-肾上腺素受体的过表达可能通过增加心肌细胞内cAMP水平改善心肌细胞的收缩功能。

关键词: &beta, 2 -肾上腺素能受体, 基因转染, 心肌细胞, 功能, 犬

Abstract:

BACKGROUND: Previous studies have found that cardiocytes overexpressing β-adrenergic receptors (β-AR) can improve its contractile function in transgenic mice, rats and other small animals, but experimental reports about the large mammals are few.
OBJECTIVE: To observe changes and mechanisms of contractile function following cardiocytes overexpressing β2-AR in canines.
METHODS: Canine cardiocytes were isolated by collagenase II and transfected by adenovirus β2AR. The expression of β2AR protein of cardiocytes, the level of intracellular cyclic adenosine monophosphate (cAMP) at basic state and maximum systolic states, and the changes of contractile function were observed at 48 hours after operation. 
RESULTS AND CONCLUSION: Compared with the control group, the expression of β2AR protein of cardiocytes and the level of the intracellular cAMP in the adenovirus β2AR group were increased (P < 0.05). The basal contraction amplitude of cadiocytes in adenovirus β2-AR group was also increased significantly (P < 0.05), but the max contraction amplitude of cardiocytes was not changed significantly between two groups. The study suggested that the over-expression of β2AR could increase the level of intracellular cAMP and improved the contractile function of cadiocytes in canines.

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