中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (8): 1517-1520.doi: 10.3969/j.issn.1673-8225.2010.08.045

• 材料生物相容性 material biocompatibility • 上一篇    

聚丙烯酰胺接枝改性聚丙烯膜的血液相容性

赵之明1, 2，彭承宏3，韩宝三3，高长有4   

  1. 1 浙江大学医学院第二附属医院外科，浙江省杭州市  310008；2 解放军总医院肝胆外科，北京市 100853；3 上海第二医科大学瑞金医院外科，上海市  200025；4 浙江大学高分子科学与工程系，浙江省杭州市  310027
  • 出版日期:2010-02-19 发布日期:2010-02-19
  • 作者简介:赵之明☆，男，1971年生，山东省德州市平原县人，满族，2005年浙江大学医学院毕业，博士，主治医师，主要从事肝移植与生物人工肝的基础与临床研究及肝胆外科微创研究。
  • 基金资助:

    国家自然科学基金资助项目(20074031)。

In vitro blood compatibility of polyacrylamide grafted polypropylene membrane

Zhao Zhi-ming 1, 2, Peng Cheng-hong 3, Han Bao-san3, Gao Chang-you4   

  1. 1 Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou   310008, Zhejiang Province, China; 2 Department of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing   100853, China; 3 Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai   200025, China; 4 Department of Polymer Science and Engineering, Zhejiang University, Hangzhou   310027, Zhejiang Province, China
  • Online:2010-02-19 Published:2010-02-19
  • About author:Zhao Zhi-ming☆, Doctor, Attending physician, Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310008, Zhejiang Province, China; Department of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing 100853, China zhimingzhao616@ 126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 20074031*

PDF

480

被引次数

8

摘要：

背景：绝大多数高分子材料在与血液接触时都导致不同程度凝血，使其应用受到限制。因此研制有优良抗凝血性能的高分子材料成为生物人工肝材料临床研究中的关键问题。
目的：体外检测新型人工肝反应器材料——聚丙烯酰胺接枝改性聚丙烯膜(PP-g-AAm)的血液相容性。
方法：对改性前、后的聚丙烯膜行溶血试验、凝血酶原时间和活化部分凝血激酶时间试验，用流式细胞术检测血小板CD62P和CD63的表达率，扫描电镜观察两种膜上血小板的黏附情况。
结果与结论：聚丙烯膜和PP-g-AAm膜的溶血率分别为1.32%和1.46%；聚丙烯膜的凝血酶原时间和活化部分凝血激酶时间较PP-g-AAm膜明显缩短(P < 0.05)；PP-g-AAm膜激活血小板表达CD62P、CD63的百分率都明显少于聚丙烯膜(P < 0.05)；扫描电镜观察两种材料表面黏附的血小板都有明显变形，但PP-g-AAm膜表面黏附的血小板明显少于聚丙烯膜。提示PP-g-AAm膜具有良好的血液相容性。

关键词: 人工肝, 血液相容性, 生物相容性, 聚丙烯, 膜生物材料

Abstract:

BACKGROUND: Contacting with blood, most of polymer materials lead to different extents of blood coagulation, which limits their clinical application. Therefore, developing polymer materials with excellent anticoagulant property has become a key to clinical study of bioartificial liver materials.
OBJECTIVE: To in vitro detect the blood compatibility of polyacrylamide grafted polypropylene (PP) membrane (PP-g-AAm), a novel artificial liver reactor material.
METHODS: Prior to and after modification, hemolytic test, prothrombin time and activated partial thromboplastin time tests of PP membrane were performed; blood platelet CD62P and CD63 expression rates were determined by flow cytometry, and platelet adhesion on PP and PP-g-AAm membranes by scanning electron microscopy.
RESULTS AND CONCLUSION: The hemolysis ratio of PP and PP-g-AAm membranes was 1.32% and 1.46%, respectively. Compared with PP-g-AAm membrane, prothrombin time and activated partial thromboplastin time of PP membrane were markedly shorter (P < 0.05). CD62P and CD63 expression rates in the PP-g-AAm membrane were significantly lower than PP membrane (P < 0.05). Scanning electron microscopy results revealed that there were obvious changes of platelets adhering to these two membranes, but platelets adhering to PP-g-AAm membrane were fewer than PP membrane. These results indicate that PP-g-AAm membrane exhibits good blood compatibility.

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