中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (34): 8921-8929.doi: 10.12307/2026.896

• 肌肉肌腱韧带组织构建 tissue construction of the muscle, tendon and ligament • 上一篇    下一篇

骨疏康颗粒对肌少-骨质疏松模型大鼠肌肉与骨骼中成肌、成骨因子的影响

魏  巍1，王德羽2，于智同2，乔春林1，崔相然2，刘洪飞2，王承博1，王世轩1，滕  海1   

  1. 1辽宁中医药大学附属第二医院，辽宁省沈阳市  110034；2辽宁中医药大学，辽宁省沈阳市  110847

  • 收稿日期:2025-09-24 修回日期:2026-02-16 出版日期:2026-12-08 发布日期:2026-04-13
  • 通讯作者: 滕海，硕士，主任医师，辽宁中医药大学附属第二医院，辽宁省沈阳市 110034
  • 作者简介:魏巍，男，1992年生，博士，讲师，主要从事中医药治疗骨质疏松、骨与关节损伤方面的研究。

Effects of Gushukang Granules on the expression of myogenic and osteogenic factors in the muscles and bones of sarcopenic osteoporosis rats

Wei Wei1, Wang Deyu2, Yu Zhitong2, Qiao Chunlin1, Cui Xiangran2, Liu Hongfei2, Wang Chengbo1, Wang Shixuan1, Teng Hai1   

  1. 1The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110034, Liaoning Province, China; 2Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning Province, China
  • Received:2025-09-24 Revised:2026-02-16 Online:2026-12-08 Published:2026-04-13
  • Contact: Teng Hai, MS, Chief physician, The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110034, Liaoning Province, China
  • About author:Wei Wei, PhD, Lecturer, The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110034, Liaoning Province, China

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摘要：



文题释义：
骨疏康颗粒：是遵循中医“补肾、益气、壮骨”理论研制的中药制剂，由淫羊藿、熟地黄、骨碎补、黄芪、木耳、黄瓜子等组成。在此次研究中，用于干预去卵巢所致的骨质疏松大鼠模型，通过灌胃方式给药，旨在调节肌肉与骨骼组织的代谢，改善因模型构建引发的肌肉萎缩和骨质流失状况。
成肌、成骨因子：成肌因子是参与肌肉生长、代谢及修复的一类因子，如此次研究中涉及的白细胞介素6、肿瘤坏死因子α、胰岛素样生长因子1 等，它们可影响肌纤维的结构与功能，在肌肉萎缩或修复过程中发挥作用；成骨因子是参与骨骼形成、重塑及代谢的因子，如此次研究中的骨钙素、胰岛素样生长因子1 等，对骨小梁的数量、排列及骨组织的正常功能维持至关重要，两类因子共同参与肌-骨交互的调控过程。

背景：肌少-骨质疏松症中肌肉减少与骨质疏松常同时存在，导致跌倒和骨折风险显著上升，骨疏康颗粒在临床用于骨质疏松症治疗，但它对肌肉与骨骼中成肌、成骨因子的作用机制尚未明确。
目的：探讨骨疏康颗粒对大鼠肌肉与骨骼中成肌、成骨因子的影响。
方法：将36只健康SD大鼠随机分为对照组、模型组、骨疏康组，每组12只，后两组采用去卵巢法建立骨质疏松大鼠模型。术后 4 周开始给药，骨疏康组予1.05 mL/kg骨疏康颗粒溶液灌胃，其余各组予等量生理盐水，1次/d，连续给药 12 周，观察各组大鼠一般情况变化。苏木精-伊红染色观察大鼠骨骼和肌肉组织形态学改变；RT-qPCR检测大鼠肌肉组织中白细胞介素6、肿瘤坏死因子 α 和骨组织中骨钙素、胰岛素样生长因子 1 mRNA 表达水平；免疫组化观察大鼠肌肉和骨组织白细胞介素6、肿瘤坏死因子 α、胰岛素样生长因子 1 和骨钙素的表达情况；Western blot检测大鼠肌肉和骨组织中白细胞介素6、肿瘤坏死因子 α、胰岛素样生长因子 1 和骨钙素的蛋白表达水平。
结果与结论：①与对照组相比，模型组呈现典型肌少骨质疏松表型，肌肉组织中肌纤维排列稀疏紊乱、间距增宽，伴随明显萎缩；骨组织中骨小梁数量减少、排列稀疏、间距增宽，可见断裂及连接性差；肌肉及骨组织中白细胞介素6、肿瘤坏死因子 α 的 mRNA 及蛋白表达显著升高(P < 0.01)，胰岛素样生长因子 1、骨钙素的 mRNA 及蛋白表达显著降低(P < 0.01)；②与模型组相比，骨疏康组上述指标明显改善，肌肉组织肌纤维排列趋于有序，间质纤维增生明显；骨组织骨小梁数量增多、连接性改善(仍较疏松)；肌肉及骨组织中白细胞介素6、肿瘤坏死因子α的 mRNA 及蛋白表达显著降低(P < 0.01)，胰岛素样生长因子 1、骨钙素的 mRNA 及蛋白表达显著升高(P < 0.01)；③Western blot结果与免疫组化趋势一致；④提示骨疏康颗粒可通过下调肌肉与骨组织中白细胞介素6、肿瘤坏死因子 α 的异常高表达，上调胰岛素样生长因子1、骨钙素的表达，改善去卵巢大鼠的肌肉萎缩与骨小梁稀疏表型，其作用可能与调控肌-骨交互信号通路、平衡炎症与促生长因子水平相关，为骨疏康颗粒用于肌少骨质疏松症的临床治疗提供了实验支持。

https://orcid.org/0000-0002-7021-9310(魏巍)


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 骨疏康颗粒, 肌少-骨质疏松症, 白细胞介素6, 肿瘤坏死因子 α, 胰岛素样生长因子1, 骨钙素

Abstract: BACKGROUND: In sarcopenic osteoporosis, muscle loss and osteoporosis often coexist, leading to a significant increase in the risk of falls and fractures. Gushukang Granules are clinically used for the treatment of osteoporosis; however, the mechanism of action on myogenic and osteogenic factors in muscle and bone remains unclear.
OBJECTIVE: To investigate the effects of Gushukang Granules on myogenic and osteogenic factors in the muscles and bones of rats.
METHODS: Thirty-six healthy Sprague-Dawley rats were randomly divided into a control group, a model group, and a Gushukang Granules group, with 12 rats in each group. Osteoporosis was induced in the latter two groups via ovariectomy. Four weeks after surgery, drug administration began. The Gushukang Granules group was given 1.05 mL/kg of Gushukang Granules solution by gavage daily, while the other groups received an equal volume of normal saline, once a day for 12 consecutive weeks. General conditions of the rats in each group were observed. Hematoxylin-eosin staining was used to examine morphological changes in the bone and muscle tissues. Real-time quantitative polymerase chain reaction was performed to measure mRNA expression levels of interleukin-6, tumor necrosis factor-α, osteocalcin, and insulin-like growth factor-1 in the muscle tissues. Immunohistochemistry was used to observe the expression of interleukin-6, tumor necrosis factor-α, insulin-like growth-1, and osteocalcin in the muscle and bone tissues. Western blot was used to detect the protein expression levels of interleukin-6, tumor necrosis factor-α, insulin-like growth 1, and osteocalcin in the muscle and bone tissues. 
RESULTS AND CONCLUSION: (1) Compared with the control group, the model group exhibited typical sarcopenic osteoporosis phenotypes: In muscle tissue, muscle fibers were sparsely and disorderly arranged with widened spacing, accompanied by significant atrophy. In bone tissue, the number of trabeculae decreased, with sparse arrangement, widened spacing, visible fractures, and poor connectivity. mRNA and protein expression of interleukin-6 and tumor necrosis factor-α were significantly increased (P < 0.01), while mRNA and protein expression of insulin-like growth 1 and osteocalcin were significantly decreased (P < 0.01) in both muscle and bone tissues. (2) Compared with the model group, the Gushukang Granules group showed significant improvements in the above indicators: In muscle tissue, the arrangement of muscle fibers tended to be more orderly, with obvious interstitial fibrosis. In bone tissue, the number of trabeculae increased, and connectivity improved (though still relatively loose). mRNA and protein expression of interleukin-6 and tumor necrosis factor-α were significantly decreased (P < 0.01), while mRNA and protein expression of insulin-like growth-1 and osteocalcin were significantly increased (P < 0.01) in both muscle and bone tissues. (3) Western blot results were consistent with the immunohistochemistry trends. Overall, these findings suggest that Gushukang Granules can improve muscle atrophy and trabecular sparseness in ovariectomized rats by downregulating the abnormally high expression of interleukin-6 and tumor necrosis factor-α and upregulating the expression of insulin-like growth-1 and osteocalcin in muscle and bone tissues. Its effects may be related to regulating the muscle-bone interaction signaling pathway and balancing inflammatory and growth-promoting factors, providing experimental support for the clinical application of Gushukang Granules in sarcopenic osteoporosis. 


Key words: Gushukang Granules, sarcopenic osteoporosis, interleukin-6, tumor necrosis factor-α, insulin-like growth factor-1, osteocalcin

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