中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (10): 2030-2037.doi: 10.12307/2025.224

• 组织工程骨材料 tissue-engineered bone • 上一篇    下一篇

羟基磷灰石-氧化石墨烯复合涂层促进大鼠骨缺损的修复

都曼别克·阿曼台,何惠宇,韩祥祯   

  1. 新疆医科大学第一附属医院(附属口腔医院)修复种植科,新疆维吾尔自治区口腔医学研究所,新疆维吾尔自治区乌鲁木齐市  830000
  • 收稿日期:2023-10-30 接受日期:2024-01-20 出版日期:2025-04-08 发布日期:2024-08-21
  • 通讯作者: 韩祥祯,硕士,主治医师,新疆医科大学第一附属医院(附属口腔医院)修复种植科,新疆维吾尔自治区口腔医学研究所,新疆维吾尔自治区乌鲁木齐市 830000
  • 作者简介:都曼别克·阿曼台,男,1997年生,新疆维吾尔自治区阜康市人,哈萨克族,新疆医科大学第一附属医院(附属口腔医院)修复种植科在读硕士,主要从事钛涂层表面改性及成骨研究。

Hydroxyapatite-graphene oxide composite coating promotes bone defect repair in rats

Dumanbieke·Amantai, He Huiyu, Han Xiangzhen   

  1. Department of Prosthodontics, First Affiliated Hospital (Affiliated Stomatological Hospital) of Xinjiang Medical University, Xinjiang Uygur Autonomous Region Institute of Stomatology, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
  • Received:2023-10-30 Accepted:2024-01-20 Online:2025-04-08 Published:2024-08-21
  • Contact: Han Xiangzhen, Master, Attending physician, Department of Prosthodontics, First Affiliated Hospital (Affiliated Stomatological Hospital) of Xinjiang Medical University, Xinjiang Uygur Autonomous Region Institute of Stomatology, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
  • About author:Dumanbieke·Amantai, Master candidate, Department of Prosthodontics, First Affiliated Hospital (Affiliated Stomatological Hospital) of Xinjiang Medical University, Xinjiang Uygur Autonomous Region Institute of Stomatology, Urumqi 830000, Xinjiang Uygur Autonomous Region, China

摘要:

文题释义:
电化学沉积:是另一种表面修饰的方法,分为电泳和沉积2步,悬浮液中的粒子在所施电场的驱动下发生电泳,粒子定向移动到电极,最终在电极表面发生粒子间的凝结形成沉积物。电化学沉积法因具有设备简单、成本低、周期短、涂层均匀等优点而受到关注,是一种广泛运用于种植体表面处理的方法。
羟基磷灰石-氧化石墨烯复合涂层:石墨烯是一种以碳原子sp2杂化形成六角形蜂巢晶格为基本单位的二维平面多环芳烃原子晶体,稳定的晶格结构使石墨烯具有高机械强度、高比表面积、高导电、高导热、低密度等优良的物理特性。羟基磷灰石化学成分与人体骨骼组织的主要无机矿物成分相似,具有良好的生物相容性和骨传导性、良好的生物活性、合适的机械强度及稳定的化学性质等特点,被广泛应用于临床医学研究。因此,氧化石墨烯和羟基磷灰石复合材料理论上是一种生物学性能优良同时兼具物理性能的新型种植体。

背景:钛及钛涂层材料在口腔种植领域广泛运用,但仍存在着种植体周围炎、种植体脱落及松动等现象,因此对纯钛的表面改性成为了口腔医学研究的热点问题。
目的:探究钛表面羟基磷灰石-氧化石墨烯复合涂层的物理性能及促成骨性能。
方法:在电压为10,30,50 V条件下,采用电化学沉积法在钛表面分别制备羟基磷灰石-氧化石墨烯复合涂层,表征涂层的微观形貌、亲水性能,筛选最佳电压条件下制备的复合涂层,用于动物实验。取54只SD大鼠,在双侧后肢股骨头制备直径2 mm、深度7 mm的缺损,随机分3组干预,每组18只:空白组不植入钛材料,纯钛组植入纯钛材料,涂层组植入负载羟基磷灰石-氧化石墨烯复合涂层钛材料,植入后第4,8,12周取材,通过X射线片、Micro-CT扫描、病理切片染色观察成骨效果。
结果与结论:①扫描电镜下可见,当电压为10 V时,涂层中出现了大量的裂纹和块状物;当电压升至30 V时,涂层仍然存在一些小的块状物,但整体上表现出较为平坦的均匀性;当电压为50 V时,涂层的分布更加均匀,裂纹和斑点也减少。在50 V电压下制备的复合涂层亲水性能最佳。综合以上,动物实验选择在50 V电压下制备的复合涂层材料。②X射线片显示种植体植入位置相对固定,未产生严重的术后炎症反应。Micro-CT扫描结果显示,涂层组种植体表面新骨形成速度与成骨量均优于纯钛组(P < 0.001)。病理切片苏木精-伊红染色、Masson染色进一步证实了Micro-CT扫描结果。病理切片免疫组化染色显示,纯钛组植入第12周的骨桥蛋白、骨形态发生蛋白2表达高于空白组(P < 0.001),涂层组植入第12周的骨桥蛋白、骨形态发生蛋白2表达高于纯钛组(P < 0.001)。③结果显示,羟基磷灰石-氧化石墨烯复合涂层材料具有良好的物理性能与促成骨性能。
https://orcid.org/0000-0001-7226-4804(都曼别克·阿曼台)

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料;口腔生物材料;纳米材料;缓释材料;材料相容性;组织工程

关键词: 氧化石墨烯, 羟基磷灰石, 电化学沉积, 复合涂层, 成骨, 种植体

Abstract: BACKGROUND: Titanium and titanium-coated materials are widely used in the field of oral implantology, but there are still phenomena such as peri-implantitis, implant loss and loosening. Therefore, the surface modification of pure titanium has become a hot topic in oral medicine research. 
OBJECTIVE: To investigate the physical and osteogenic properties of hydroxyapatite-graphene oxide composite coating on titanium surface.
METHODS: Hydroxyapatite-graphene oxide composite coatings were prepared on the titanium surface by electrodeposition at voltages of 10, 30, and 50 V. The micromorphology and hydrophilic properties of the coatings were characterized, and the composite coatings prepared under the optimal voltage conditions were screened for animal experiments. Fifty-four SD rats were selected to prepare defects of 2 mm in diameter and 7 mm in depth on the femoral head of both hind limbs, and were randomly divided into 3 groups with 18 rats in each group: no titanium material was implanted in the blank group; pure titanium material was implanted in the pure titanium group, and coated titanium material loaded with hydroxyapatite-graphene oxide composite coating was implanted in the coated group. The osteogenesis effect was observed by X-ray, Micro-CT scan, and pathological section staining at 4, 8, and 12 weeks after implantation. 
RESULTS AND CONCLUSION: (1) Under scanning electron microscopy, when the voltage was 10 V, there were a lot of cracks and clumps in the coating. When the voltage rose to 30 V, there were still some small clumps in the coating, but the overall uniformity was relatively flat. When the voltage was 50 V, the coating was more evenly distributed and cracks and spots were reduced. The hydrophilicity of the composite coating prepared at 50 V voltage was the best. In summary, the composite coating material prepared at 50 V voltage was selected in animal experiments. (2) The X-ray film showed that the implant position was relatively fixed, and no serious postoperative inflammation occurred. The results of Micro-CT scan showed that the new bone formation rate and bone formation volume on the implant surface of the coated group were better than those of the pure titanium group (P < 0.001). The results of Micro-CT scan were further confirmed by hematoxylin-eosin staining and Masson staining in pathological sections. Immunohistochemical staining of pathological sections showed that the expressions of osteopontin and bone morphogenetic protein 2 in the pure titanium group were higher than those in the blank group at week 12 after implantation (P < 0.001), and those in the coated group were higher than those in the pure titanium group at week 12 after implantation (P < 0.001). (3) The results show that the hydroxyapatite-graphene oxide composite coating material has good physical and osteogenic properties.

Key words: graphene oxide, hydroxyapatite, electrodeposition, composite coating, osteogenesis, implant

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