中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (21): 3811-3814.doi: 10.3969/j.issn.1673-8225.2011.21.004

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

壳聚糖-酪蛋白磷酸肽修饰钛合金表面的成骨细胞黏附与增殖

王  琳1，卜银忠1，王金清2，杨生荣2，刘  斌1   

  1. 1兰州大学口腔医学院，甘肃省兰州市 730000
    2中国科学院兰州化学物理研究所固体润滑国家重点实验室，甘肃省兰州市  730000
  • 收稿日期:2010-11-09 修回日期:2010-12-10 出版日期:2011-05-21 发布日期:2011-05-21
  • 通讯作者: 刘斌，博士，教授，兰州大学口腔医学院，甘肃省兰州市 730000 liubkq@lzu.edu.cn 并列通讯作者：王金清，博士，研究员，中国科学院兰州化学物理研究所，甘肃省兰州市 730000 jqwang@lzb.ac.cn
  • 作者简介:王琳★，女，1984年生，甘肃省兰州市人，汉族，兰州大学口腔医学院在读硕士，主要从事口腔修复和生物材料学方面的研究。 walkiewl@163.com
  • 基金资助:

    国家自然科学基金项目(50801065)；中国科学院“百人计划”资助项目；兰州市科技局科技计划项目(2008-1-86)；中国科学院兰州化学物理研究所固体润滑国家重点实验室开放项目(0808)。

Adhesion and proliferation of osteoblasts on the surface of titanium alloy modified by chitosan-casein phosphopeptides

Wang Lin1, Bu Yin-zhong1, Wang Jin-qing2, Yang Sheng-rong2, Liu Bin1   

  1. 1School of Stomatology, Lanzhou University, Lanzhou   730000, Gansu Province, China
    2National State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou   730000, Gansu Province, China
  • Received:2010-11-09 Revised:2010-12-10 Online:2011-05-21 Published:2011-05-21
  • Contact: Liu Bin, Doctor, Professor, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China liubkq@lzu.edu.cn Correspondence to: Wang Jin-qing, Doctor, Investigator, National State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu Province, China jqwang@lzb.ac.cn
  • About author:Wang Lin★, Studying for master’s degree, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China walkiewl@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 50801065*; 100 Talents Program of the Chinese Academy of Sciences*; Science and Technology Planning Project of Lanzhou Technology Bureau, No. 2008-1-86*; Open Program of National State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, No. 0808*

PDF

431

被引次数

6

摘要：

背景：钛合金表面只有吸附某些有机分子才能使生长因子活化，促进细胞增殖，从而激活周围的骨细胞发挥作用。
目的：在钛合金种植体表面制备生物活性涂层，寻找一种有效促进骨整合的方法。
方法：采用多步组装方法在硅烷偶联剂修饰的钛合金表面依次接枝壳聚糖与0，5，10 g/L的酪蛋白磷酸肽，形成生物活性复合涂层，以未修饰的钛合金为对照。将经过不同处理的钛合金与成骨细胞共培养，利用细胞计数法、荧光染色法、MTT比色法评价涂层的生物学性能。
结果与结论：与未修饰的钛合金相比，在壳聚糖上接枝酪蛋白磷酸肽后，钛合金表面成骨细胞的早期黏附与增殖明显提高  (P < 0.05)，并且随酪蛋白磷酸肽质量浓度的增大促进作用显著增强(P < 0.05)。说明制备的壳聚糖-酪蛋白磷酸肽复合涂层可以显著提高成骨细胞的功能，增强钛合金的生物学性能，有望成为有效促进骨整合的方法。

关键词: 钛合金, 壳聚糖, 酪蛋白磷酸肽, 成骨细胞, 生物相容性

Abstract:

BACKGROUND: The surface of titanium alloy only absorbs some organic molecule to activate the growth factor and stimulate cell proliferation, thereby activating the surrounding bone cells and producing a marked effect.
OBJECTIVE: To prepare a bioactive coating on the surface of the titanium alloy implant, and to evaluate one promising strategy to enhance osseointegration.
METHODS: The surface of titanium alloy modified by silane coupling agent was covalently grafted with chitosan (CS) and the concentration of 0, 5, 10 g/L casein phosphopeptides (CPP) in turn to form composite coatings using multi-step assembly method, compared with unmodified titanium alloy. The biocompatibility of the fabricated coatings was evaluated through co-culture of the titanium alloy and osteoblasts that modified through different procedures, using cell counting, fluorescence staining, and MTT colorimetric assay.
RESULTS AND CONCLUSION: Compared with the titanium alloy without modification, the early adhesion and proliferation of surface of titanium alloy were significantly increased after CS covalently grafted with CPP (P < 0.05). Moreover, with the increase of CPP concentration, the promotion enhanced significantly (P < 0.05). (The osteoblasts on the surface of titanium alloy without modification displayed smooth surface, clear boundary, not fully stretched; the osteoblast cell body of the surface of titanium alloy increased significantly, rough surface, fuzzy boundaries, and fully stretched after modification by CS -CPP composite coating). It is indicated that the prepared CS-CPP composite coating can significantly increased the function of osteoblasts, improve biological properties of titanium alloy, and expected to be an effective method to promote osseointegration.

中图分类号: