中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (21): 3807-3810.doi: 10.3969/j.issn.1673-8225.2011.21.003

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

β-磷酸三钙/聚-L-乳酸复合材料的骨内降解

王  晓1,2，刘玉杰1，陈  旭1   

  1. 1解放军总医院骨科六区，北京市 100853
    2解放军第62医院骨科，云南省普洱市 665000
  • 收稿日期:2011-02-12 修回日期:2011-04-11 出版日期:2011-05-21 发布日期:2011-05-21
  • 通讯作者: 刘玉杰，博士生导师，主任医师，解放军总医院骨科六区，北京市 100853 Liuyj301yy@yahoo.com.cn
  • 作者简介:王晓★，男，1984年生，山西省运城市人，汉族，2006年解放军第四军医大学毕业，硕士，主治医师，主要从事关节镜微创外科与运动医学方面的研究。 kinomorn@yahoo.com.cn

In vivo degradation of beta-tricalcium phosphate/poly-l-lactide composites in cancellous bone

Wang Xiao1,2, Liu Yu-jie1, Chen Xu1   

  1. 1Department of Orthopedics, Chinese PLA General Hospital, Beijing  100853, China
    2Department of Orthopedics, the 62 Hospital of Chinese PLA, Puer  665000, Yunnan Province, China
  • Received:2011-02-12 Revised:2011-04-11 Online:2011-05-21 Published:2011-05-21
  • Contact: Liu Yu-jie, Chief physician, Doctoral supervisor, Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China Liuyj301yy@yahoo.com.cn
  • About author:Wang Xiao★, Master, Attending physician, Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China; Department of Orthopedics, the 62 Hospital of Chinese PLA, Puer 665000, Yunnan Province, China kinomorn@yahoo.com.cn

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摘要：

背景：调控聚乳酸类可吸收材料的降解速率，使材料降解与新生骨爬行替代速度更同步，加入羟基磷灰石或β-磷酸三钙等无机粒子是目前的主流选择。
目的：对比观察β-磷酸三钙/聚-L-乳酸复合材料和聚-L-乳酸在松质骨内的降解速度及诱导成骨能力。
方法：将β-磷酸三钙/聚-L-乳酸复合材料及聚-L-乳酸材料分别植入12只新西兰大白兔双侧股骨内髁及外髁后，于术后6，12，24周3个时间点取材，测定其各个时间点的生物吸收率，扫描电镜和光学显微镜观察其在松质骨内的形态学变化，周围新骨爬行替代及异物反应情况。
结果与结论：各个时间点β-磷酸三钙/聚-L-乳酸与周围骨质贴合比聚-L-乳酸材料更紧密，未见明显异物反应。6周时β-磷酸三钙/聚-L-乳酸材料生物吸收率小于聚-L-乳酸材料，12周后生物吸收率增速加快，同时材料表面出现均匀分布的微孔及裂隙；术后24周内两种材料均未见新生骨爬行替代。结果表明β-磷酸三钙/聚-L-乳酸复合材料早期降解较聚-L-乳酸材料慢，有利于移植物植入早期的坚强固定；6周后降解加快，24周内未见诱导成骨现象。

关键词: 聚-L-乳酸, &beta, -磷酸三钙/聚-L-乳酸, 生物可吸收材料, 体内降解, 诱导成骨

Abstract:

BACKGROUND: To improve the degradation and osteoinductive property of Poly-L-lactide (PLLA) materials, adding inorganic particles such as hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) is a popular choice.
OBJECTIVE: To investigate the in vivo degradation and osteoinductive property of β-TCP/PLLA and PLLA materials in cancellous bone comparatively.
METHODS: Totally 12 adult New Zealand rabbits were implanted PLLA andβ-TCP/PLLA materials into medial and lateral femoral condyle on both sides respectively. The rabbits were sacrificed separately at 6, 12, 24 weeks after operation, 4 animals at each time. The bioabsorption rate, light microscopic and scanning electron microscopic images of PLLA and β-TCP/PLLA materials and surrounding bony tissues were investigated.
RESULTS AND CONCLUSION: β-TCP/PLLA composites contacted more closely with surrounding bone tissues than PLLA. No foreign body reaction was observed. The bioabsorption rate of β-TCP/PLLA that was slower than PLLA in the first 6 weeks accelerated after this period, while more micropores and fine groove in the surfaces and inner part of β-TCP/PLLA composites were observed. No bony replacement was observed in 24 weeks postoperatively. The results show that the degradation rate of β-TCP /PLLA composites that are slower than PLLA in early stage speed up after this period, which is propitious to stablize fixation of implants. Tissue compatibility of β-TCP /PLLA composites is better than PLLA. However, no osteoinductive property of both materials is observed during the study periods.

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