中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (24): 4383-4386.doi: 10.3969/j.issn.1673-8225.2011.24.004

• 骨组织构建 bone tissue construction • 上一篇    下一篇

腭中缝牵张成骨中骨形成蛋白2的表达

胡海琨1，周  静2，王  尧1，李  婧1，何武林1，邹淑娟1   

  1. 1口腔疾病国家重点实验室，四川大学华西口腔医院正畸科，四川省成都市  610041
    2昆明市延安医院口腔科，云南省昆明市  650031
  • 收稿日期:2010-12-19 修回日期:2011-02-23 出版日期:2011-06-11 发布日期:2011-06-11
  • 通讯作者: 邹淑娟，博士，教授，博士生导师，口腔疾病国家重点实验室，四川大学华西口腔医院正畸科，四川省成都市 610041 shujuanzou@yahoo.com.cn
  • 作者简介:胡海琨☆，女，1985年生，四川省成都市人，汉族，四川大学在读博士，主要从事颅面缝生长发育及相关畸形的组织工程治疗的研究。 helen-8591@ 163.com
  • 基金资助:

    国家自然科学基金资助项目(30772454)；四川省科技厅攻关项目(2006z09-013)。

Expression of bone morphogenetic protein-2 during the distraction process in midpalatal suture of rats

Hu Hai-kun1, Zhou Jing2, Wang Yao1, Li Jing1, He Wu-lin1, Zou Shu-juan1   

  1. 1State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu  610041, Sichuan Province, China
    2Department of Stomatology, Yan’an Hospital, Kunming  650031, Yunnan Province, China
  • Received:2010-12-19 Revised:2011-02-23 Online:2011-06-11 Published:2011-06-11
  • Contact: Zou Shu-juan, Doctor, Professor, Doctoral supervisor, State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China shujuanzou@yahoo. com.cn
  • About author:Hu Hai-kun☆, Studying for doctorate, State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China helen-8591@163. com
  • Supported by:

     the National Natural Science Foundation of China, No. 30772454*; the Tackle Key Program of Sichuan Provincial Science and Technology Bureau, No. 2006z09-013*

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318

被引次数

4

摘要：

背景：正畸医生常常通过扩大腭中缝矫正上颌骨横向发育不足。骨形成蛋白2可以诱导骨和软骨的形成，促进牵张成骨过程中的骨重建。然而关于骨形成蛋白2在腭中缝牵张成骨中的时间空间表达规律尚不清楚。
目的：观察骨形成蛋白2在大鼠腭中缝牵张成骨过程中的表达规律。
方法：实验选用80只5周龄雄性Wistar大鼠，分为实验组和对照组(包括阴性对照和空白对照)。将初始力值为50 g的腭中缝扩大簧黏接到大鼠两侧上颌牙列上建立大鼠腭中缝牵张模型，牵张1，4，7，14 d后，采用免疫组织化学和实时定量荧光PCR方法分析骨形成蛋白2蛋白和mRNA在各加力时间点的表达。
结果与结论：腭中缝扩张后骨形成蛋白2蛋白表达水平显著增加，且存在时空表达差异，主要定位于腭中缝纤维组织、软骨细胞层、骨细胞和成骨细胞胞浆及其细胞外基质。同时，骨形成蛋白2 mRNA表达也明显上调。提示腭中缝牵张力可刺激骨缝中骨形成蛋白2蛋白和mRNA的合成，在骨缝塑建过程中发挥重要作用。

关键词: 腭中缝, 牵张成骨, 骨形成蛋白2, 骨组织工程, 大鼠

Abstract:

BACKGROUND: Orthodontists usually use palatal suture expansion to solve the problem of maxillofacial deformity. Some studies have shown that bone morphogenetic protein-2 (BMP-2) can induce bone and cartilage formation, promote the distraction osteogenesis in the process of bone remodeling under mechanical tension forces. However, underlying mechanisms of BMP-2 that drive bone formation during palatal suture expansion remain unknown.
OBJECTIVE: To investigate the law of BMP-2 expression in mid-palatal suture remodeling after subjecting to tensional strain loading.
METHODS: Wistar male rats of 5 weeks old, with average weight of 70-80 g, were randomly divided into 2 groups, separately, Rats in experimental group were placed on the expansion appliance, with opening loops with an initial force of 50 g applied to mid-palatal suture for periods of 1, 4 ,7 and 14 days. After expansion, the mid-palatal suture was observed by IHC staining and real time-PCR to investigate the expression of BMP-2 protein and mRNA during mid-palatal suture remodeling after suture expansion.
RESULTS AND CONCLUSION: Sections with IHC staining revealed BMP-2 was detected in the osteoblast, osteocytes, chondrocytes, also in the connective tissue cells of suture. Coincident with IHC, the result of real-time PCR demonstrated time-differential expression of the mRNA that was significantly higher than that of controls at all-time points during the expansion suture remodeling. It is suggesting that BMP-2 was susceptible to mechanical tension forces and may play an important role in the remodeling of mid-palate suture.

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