中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (21): 3839-3842.doi: 10.3969/j.issn.1673-8225.2011.21.009

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

细胞培养法评价镁合金能化型共聚酯涂层的生物相容性

潘  锋1,2，柏树令1   

  1. 中国医科大学，1解剖教研室，2生物材料研究室，辽宁省沈阳市 110001
  • 收稿日期:2011-02-09 修回日期:2011-03-15 出版日期:2011-05-21 发布日期:2011-05-21
  • 作者简介:潘锋☆，男，1976年生，辽宁省沈阳市人，汉族，中国科学院金属研究所访学博士，副教授，主要从事医用降解性镁合金的开发和应用研究。 pandaifeng@sina.com
  • 基金资助:

    国家自然科学基金资助项目(81000791)；辽宁省教育厅高校资助项目(L2010580)。

Biocompatibility of energied copolyesters coating for magnesium alloy evaluated by cell culture assay

Pan Feng1, 2, Bai Shu-ling1   

  1. 1Department of Anatomy, 2Department of Biological Materials, China Medical University, Shenyang  110001, Liaoning Province, China
  • Received:2011-02-09 Revised:2011-03-15 Online:2011-05-21 Published:2011-05-21
  • About author:Pan Feng☆, Associate professor, 1Department of Anatomy, 2Department of Biological Materials, China Medical University, Shenyang 110001, Liaoning Province, China pandaifeng@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81000791*; Liaoning Provincial Project in Higher Education Institutions of Educational Committee, No. L2010580*

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摘要：

背景：与聚乳酸、聚羟基乙酸和乳酸-羟基乙酸共聚物等生物材料相比，3-羟基丁酸和3-羟基己酸共聚酯结构多元化，具有更好的生物相容性能、生物可降解性和热加工性能。
目的：采用细胞培养法评估含不同质量浓度阿魏酸的3-羟基丁酸和3-羟基己酸共聚酯涂层的生物相容性。
方法：室温下使用乙酸乙酯配制含0(对照组)，50，100，130，150 g/L阿魏酸的3-羟基丁酸和3-羟基己酸共聚酯，观察成膜外观。通过MTT吸光度检测、荧光显微镜观察以及扫描电镜观察方法对比评估骨髓间充质干细胞在其表面的生长状态。
结果与结论：含50 g/L阿魏酸的3-羟基丁酸和3-羟基己酸共聚酯成膜后外观均匀半透明，未见白色结晶析出物，含100，130，150 g/L阿魏酸的3-羟基丁酸和3-羟基己酸共聚酯在成膜后均出现点状白色结晶物。MTT吸光度检测表明含50，100 g/L阿魏酸的3-羟基丁酸和3-羟基己酸共聚酯均能较好地促进骨髓间充质干细胞生长；荧光显微镜及扫描电镜观察均见骨髓间充质干细胞能够在含50，100 g/L阿魏酸的3-羟基丁酸和3-羟基己酸共聚酯表面黏附生长。说明含50 g/L阿魏酸的3-羟基丁酸和3-羟基己酸共聚酯最适合作为镁合金的涂层材料。

关键词: 阿魏酸, 3-羟基丁酸和3-羟基己酸共聚酯, 镁合金, 涂层, 生物相容性

Abstract:

BACKGROUND: Compared with polylactic acid (PLA), polyglycolic acid (PGA) and PLA-PGA, 3-hydroxybutyrate-co-3 - hydroxyhexanoate copolyesters (PHBHHx) has structural diversity, good biocompatibility, biodegradability and hot-working character.
OBJECTIVE: To evaluate the biocompatibility of PHBHHx at different ferulic acid mass concentration by cell culture assay.
METHODS: Acetic ether was used to prepare films with 0 (control group), 50, 100, 130, 150 g/L ferulic acid PHBHHx and the appearance of films was observed. MTT test, fluorescence microscope and scanning electron microscope were used to assess the growth of bone marrow mesenchymal stem cells surface.
RESULTS AND CONCLUSION: PHBHHx with 50 g/L ferulic acid showed a lucent film without white crystalline precipitates, but obvious white crystalline precipitates distributed in films formed by PHBHHx with 100, 130, 150 g/L ferulic acid. MTT test indicated PHBHHx with 50, 100 g/L ferulic acid could better promote the growth of bone marrow mesenchymal stem cells. The adhension of bone marrow mesenchymal stem cells in the surface of PHBHHx with 50g/L ferulic acid could be seen under fluorescence microscope and scanning electron microscope. PHBHHx with 50 g/L ferulic acid is the most appropriate coating for magnesium alloys.

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