中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (16): 3028-3032.doi: 10.3969/j.issn.1673-8225.2010.16.044

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

聚乙醇酸-乳酸共聚物复合Ⅱ型胶原和生长因子体内构建组织工程软骨

熊高鑫1，查振刚1，谭文成2，吴  昊1，李劼若1，林宏生1，夏吉生△3，黃馨霈2，屠  美4，张嘉晴5   

  1. 1暨南大学附属第一医院骨科，广东省广州市  510630；2澳门仁和医疗中心  999078；3澳门仁伯爵综合医院  999078；4暨南大学理工学院，广东省广州市  510630；5暨南大学医学院，广东省广州市  510630
  • 出版日期:2010-04-16 发布日期:2010-04-16
  • 通讯作者: 查振刚，主任医师，教授，博士生导师，暨南大学附属第一医院骨科，广东省广州市 510630
  • 作者简介:熊高鑫★，男，1984年生，安徽省池州市人，汉族，暨南大学在读硕士，主要从事关节外科与骨组织工程的研究。
  • 基金资助:

    科技部“863”计划(2007AA09Z440)；澳门科学技术发展基金项目(031/2009/A)；广州市科技计划项目(2008Z1-E411)。

Construction of tissue engineered cartilage in vivo with poly(lactide-co-glycolic acid) composited with collagen II and growth factors

Xiong Gao-xin1, Zha Zhen-gang1, Tan Wen-cheng2, Wu Hao1, Li Jie-ruo1, Lin Hong-sheng1,Xia Ji-sheng△3, Huang Xin-pei2, Tu Mei4, Zhang Jia-qing5   

  1. 1 Department of Orthopedics, First Hospital, Jinan University, Guangzhou   510630, Guangdong Province, China; 2 Renhe Medical Center, Macau   999078, China;  3 Hospital of Centre S.Januario, Macau   999078, China; 4 College of Science and Engineering at Jinan University, Guangzhou   510630, Guangdong Province, China;  5 Medical College of Jinan University, Guangzhou   510630, Guangdong Province, China
  • Online:2010-04-16 Published:2010-04-16
  • Contact: Zha Zhen-gang, Professor, Chief physician, Doctoral Supervisor, Department of Orthopedics, First Hospital, Jinan University, Guangzhou 510630, Guangdong Province, China
  • About author:Xiong Gao-xin★, Studying for master’s degree, Department of Orthopedics, First Hospital, Jinan University, Guangzhou 510630, Guangdong Province, China jiujiu12345678@sina.com
  • Supported by:

    National High-Tech Research and Development Program of China, No.2007AA09Z440*; the Science and Technology Development Fund of Macau of China, No.031/2009/A*; Science and Technology Plan Program of Guangzhou City, No. 2008Z1-E411*

PDF

401

被引次数

3

摘要：

背景：软骨组织工程的发展为处理关节软骨损伤提供了新的思路和方法，使体内构建组织工程软骨得以实现。
目的：观察骨髓基质干细胞种植到复合胶原和生长因子的聚乙醇酸-乳酸共聚物(poly- lactide-co-glycolic acid，PLGA)生物材料，再种植到大鼠体内构建组织工程软骨组织的可行性。
方法：相分离法制作PLGA，复合Ⅱ型胶原和碱性成纤维细胞生长因子，转化生长因子β1。将第3代的骨髓基质干细胞种植到复合材料上。36只SD大鼠随机分为实验组、对照组、空白组，分别于肌袋内植入骨髓基质干细胞/复合生长因子和胶原的PLGA、复合生长因子和胶原的PLGA、复合胶原的PLGA，于术后第4，8，12周取材观察细胞的定向分化及生长情况，包括大体观察、苏木精-伊红染色、甲苯胺蓝染色、Ⅱ型胶原染色、扫描电镜观察。
结果与结论：大体观察可见实验组材料有类软骨样组织生长，而对照组和空白组则仅见纤维组织生长。各种染色及电镜观察显示：实验组复合物内可见多的成软骨细胞及少量的破骨细胞。实验组甲苯胺蓝染色和Ⅱ型胶原染色为阳性，对照组和空白组均为阴性。从而证明胶原修饰的PLGA生物材料具有较好的细胞相容性；骨髓基质干细胞种植到复合胶原和生长因子的PLGA生物材料上在大鼠体内可构建组织工程软骨复合组织。

关键词: 骨髓基质干细胞, 聚乙醇酸-乳酸共聚物, 软骨向诱导, 复合组织, 组织工程

Abstract:

BACKGROUND: The development of cartilage tissue engineering provides novel ideas for treatment of articular cartilage defects and implements construction of tissue-engineered cartilage in vivo.
OBJECTIVE: To investigate the feasibility of constructing tissue-engineered osteochondral composite through bone marrow stem cells(BMSCs) cultured on the poly(lactide-co-glycolic acid) (PLGA), which was modified with collagen and cellular growth factors.

METHODS: PLGA was made by phase separation technique, composited with collagen II, basic fibroblast growth factor, and transforming growth factor-β1. The BMSCs of passage 3 were cultured on the above scaffolds. Thirty-six SD rats were randomly divided into experimental, control, and blank groups. These three groups received implantation of BMSCs composited with growth factors and collagen-PLGA, implantation of BMSCs composited with collagen-PLGA, and implantation of collagen-PLGA into the muscle, respectively. At 4, 8, and 12 weeks after surgery, cell directional differentiation and growth were examined by gross observation, hematoxylin-eosin staining, toluidine blue staining, collagen II staining, and scanning electron microscope.
RESULTS AND CONCLUSION: Gross observation showed that there were many chondroid tissues in the experimental group and fibrous tissues in the control and black groups. Stainings and electron microscope revealed that many chondroblasts and a few osteoclasts appeared in the composite of the experimental group. Toluidine blue and collagen II stainings were positive in the experimental group and negative in the control and blank groups. These findings demonstrate that PLGA modified with collagen had a good cellular compatibility. BMSCs cultured on PLGA, which was modified with collagen and cellular growth factors, can construct the tissue-engineered osteochondral composite in rats.

中图分类号: